Amphibian organ culture

Résumé Les auteurs passent en revue les problèmes étudiés et les méthodes utilisées jusqu'à ce jour pour la préparation des tissus des amphibiens en culture organotypiques. Ils envisagent en outre les possibilités qui s'offrent aux recherches futures.     

In vitro culture of larval amphibian erythroblasts

Summary A larval erythroblast culture method is described. By this method, it is possible to cultivate for several weeks a homogeneous population of cells (5·10⁵ cells/ml medium on average after 4 or 5 days of culture), which are relatively synchronous with regard to their state of differentiation.     

In vitro culture of larval amphibian erythroblasts.

A larval erythroblast culture method is described. By this method, it is possible to cultivate for several weeks a homogeneous population of cells (5-10(5) cells/ml medium on average after 4 or 5 days of culture), which are relatively synchronous with regard to their state of differentiation.     

Detection ofγ-crystallins in the developing amphibian lens by peroxidase-labelled antibodies

Zusammenfassung Antikörper, die entweder mit Fluoresceinisothiocyanat oder mit Peroxidase markiert waren, wurden auf ihre Spezifität im Erkennen von Linsen-Antigenen untersucht. Beide Markierer ergeben gleiche Resultate: die-Kristalline können in den Linsenfaserzellen lokalisiert werden, nicht aber in den Linsenepithelzellen.

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Supported by the Damon Runyon Memorial Fund for Cancer Research, New York; and by the Kredit zur Förderung des akademischen Nachwuchses an der Universität Zürich.

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This research was sponsored by the U.S. Atomic Energy Commission under contract with the Union Carbide Corporation.     

A simplified method of nerve organ culture

Résumé Une méthode simplifiée de culture d'organe nerveux est décrite. Elle réduit le temps consacré à la nourriture et permet un changement rapide du milieu nutritif. Elle convient aux études microscopique (m. à contraste de phase) et elle est relativement peu coûteuse.     

Autoradiographic study of cartilage differentiation in organ culture

Zusammenfassung Aufnahme und Verhalten von³⁵-S-Sulfat wurde in einer Organkultur sich differenzierender Somiten von Hühner-und Mäuse-Embryonen untersucht. Die Vorknorpelzellen zeigten den grössten Isotopeneinbau im Vergleich zu andern mesodermalen Zellen. Nach Vollendung der Knorpeldifferenzierung fand sich das von den Vorknorpelzellen aufgenommene Isotop in der interzellulären Matrix.

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On leave of absence from the Zoological Institute, Faculty of Science, University of Kyoto (Japan).     

The isolation,culture and organ differentiation from mesophyll protoplasts ofMollugo nudicaulis Lam., a C4 species

Summary Isolation and culture of mesophyll protoplasts fromMollugo nudicaulis, a C₄ species, is reported. Protoplasts developed into callus with root formation. However, no shoot was differentiated. This work was carried out as a part of our overall objective of inducing somatic fusion between the protoplasts of C₃ and C₄ species ofMollugo.     

Tetracyclines inhibit parathyroid hormone-induced bone resorption in organ culture

Summary Several tetracyclines (minocycline, doxycycline, tetracycline), in levels approximating physiologic concentrations, were found to inhibit parathyroid hormone-induced bone resorption in organ culture; the specificity of this effect was demonstrated by comparison with other (non-tetracycline) types of antibiotics. The ability of tetracyclines to inhibit bone resorption is consistent with the recent proposal by Golub et al². that these antibiotics can inhibit mammalian collagenolytic enzymes by a mechanism unrelated to the drug's antibacterial efficacy, a property which could be therapeutically useful in diseases characterized by excessive collagen breakdown.     

Tetracyclines inhibit parathyroid hormone-induced bone resorption in organ culture

Several tetracyclines (minocycline, doxycycline, tetracycline), in levels approximating physiologic concentrations, were found to inhibit parathyroid hormone-induced bone resorption in organ culture; the specificity of this effect was demonstrated by comparison with other (non-tetracycline) types of antibiotics. The ability of tetracyclines to inhibit bone resorption is consistent with the recent proposal by Golub et al. that these antibiotics can inhibit mammalian collagenolytic enzymes by a mechanism unrelated to the drug's antibacterial efficacy, a property which could be therapeutically useful in diseases characterized by excessive collagen breakdown.     

Retrospective immunofluorescence of specific antigens in stained and balsam embedded sections of the developing amphibian lens

A modification of the indirect immunofluorescent method is proposed for the detection of specific proteins in sections of the developing amphibian lens that were attached using egg albumin, stained and embedded into Canada balsam.     

Retrospective immunofluorescence of specific antigens in stained and balsam embedded sections of the developing amphibian lens

Summary A modification of the indirect immunofluorescent method is proposed for the detection of specific proteins in sections of the developing amphibian lens that were attached using egg albumin, stained and embedded into Canada balsam.Acknowlegments. We thank Dr G. G. Gauze and Dr Olga Hoperskaya for their help in translation of this paper.     

Response of neonatal mouse lung in organ culture to silica

Summary Short term organ cultures of lung fragments from neonatal mice maintain their normal architecture. Cultures exposed to silica flour in varying concentrations show effects resembling those seen in chronic silicosis produced by inoculation and inhalation methods.     

Fetal rat brain hemisphere tissue in nonadherent stationary organ culture

Summary A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological differentiation and the formation of a layered architecture seem to mimic some of the phenomena occurring in the developing rat brain in vivo. The fragments may therefore be a useful organ culture model for nervous tissue.     

Fetal rat brain hemisphere tissue in nonadherent stationary organ culture

A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological differentiation and the formation of a layered architecture seem to mimic some of the phenomena occurring in the developing rat brain in vivo. The fragments may therefore be a useful organ culture model for nervous tissue.     

Regression of genetically determined polycystic kidney disease in murine organ culture

Cystic kidneys from the mutant CPK strain of C57BL/6J mice were cultured in serum-free organ culture. During 120 h of incubation in chemically-defined medium, CPK cystic tubular changes underwent complete regression. Environmental factors regulate the expression of genetically determined polycystic kidney disease in this model.