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Résumé Les auteurs passent en revue les problèmes étudiés et les méthodes utilisées jusqu'à ce jour pour la préparation des tissus des amphibiens en culture organotypiques. Ils envisagent en outre les possibilités qui s'offrent aux recherches futures.  相似文献   

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Amphibian organ culture   总被引:1,自引:0,他引:1  
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Summary A larval erythroblast culture method is described. By this method, it is possible to cultivate for several weeks a homogeneous population of cells (5·105 cells/ml medium on average after 4 or 5 days of culture), which are relatively synchronous with regard to their state of differentiation.  相似文献   

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A M Duprat  M Flavin 《Experientia》1976,32(12):1587-1589
A larval erythroblast culture method is described. By this method, it is possible to cultivate for several weeks a homogeneous population of cells (5-10(5) cells/ml medium on average after 4 or 5 days of culture), which are relatively synchronous with regard to their state of differentiation.  相似文献   

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Zusammenfassung Antikörper, die entweder mit Fluoresceinisothiocyanat oder mit Peroxidase markiert waren, wurden auf ihre Spezifität im Erkennen von Linsen-Antigenen untersucht. Beide Markierer ergeben gleiche Resultate: die-Kristalline können in den Linsenfaserzellen lokalisiert werden, nicht aber in den Linsenepithelzellen.

Supported by the Damon Runyon Memorial Fund for Cancer Research, New York; and by the Kredit zur Förderung des akademischen Nachwuchses an der Universität Zürich.

This research was sponsored by the U.S. Atomic Energy Commission under contract with the Union Carbide Corporation.  相似文献   

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Résumé Une méthode simplifiée de culture d'organe nerveux est décrite. Elle réduit le temps consacré à la nourriture et permet un changement rapide du milieu nutritif. Elle convient aux études microscopique (m. à contraste de phase) et elle est relativement peu coûteuse.  相似文献   

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Zusammenfassung Aufnahme und Verhalten von35-S-Sulfat wurde in einer Organkultur sich differenzierender Somiten von Hühner-und Mäuse-Embryonen untersucht. Die Vorknorpelzellen zeigten den grössten Isotopeneinbau im Vergleich zu andern mesodermalen Zellen. Nach Vollendung der Knorpeldifferenzierung fand sich das von den Vorknorpelzellen aufgenommene Isotop in der interzellulären Matrix.

On leave of absence from the Zoological Institute, Faculty of Science, University of Kyoto (Japan).  相似文献   

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Summary Isolation and culture of mesophyll protoplasts fromMollugo nudicaulis, a C4 species, is reported. Protoplasts developed into callus with root formation. However, no shoot was differentiated. This work was carried out as a part of our overall objective of inducing somatic fusion between the protoplasts of C3 and C4 species ofMollugo.  相似文献   

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Several tetracyclines (minocycline, doxycycline, tetracycline), in levels approximating physiologic concentrations, were found to inhibit parathyroid hormone-induced bone resorption in organ culture; the specificity of this effect was demonstrated by comparison with other (non-tetracycline) types of antibiotics. The ability of tetracyclines to inhibit bone resorption is consistent with the recent proposal by Golub et al. that these antibiotics can inhibit mammalian collagenolytic enzymes by a mechanism unrelated to the drug's antibacterial efficacy, a property which could be therapeutically useful in diseases characterized by excessive collagen breakdown.  相似文献   

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Summary Several tetracyclines (minocycline, doxycycline, tetracycline), in levels approximating physiologic concentrations, were found to inhibit parathyroid hormone-induced bone resorption in organ culture; the specificity of this effect was demonstrated by comparison with other (non-tetracycline) types of antibiotics. The ability of tetracyclines to inhibit bone resorption is consistent with the recent proposal by Golub et al2. that these antibiotics can inhibit mammalian collagenolytic enzymes by a mechanism unrelated to the drug's antibacterial efficacy, a property which could be therapeutically useful in diseases characterized by excessive collagen breakdown.  相似文献   

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Summary A modification of the indirect immunofluorescent method is proposed for the detection of specific proteins in sections of the developing amphibian lens that were attached using egg albumin, stained and embedded into Canada balsam.Acknowlegments. We thank Dr G. G. Gauze and Dr Olga Hoperskaya for their help in translation of this paper.  相似文献   

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A modification of the indirect immunofluorescent method is proposed for the detection of specific proteins in sections of the developing amphibian lens that were attached using egg albumin, stained and embedded into Canada balsam.  相似文献   

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