南丰蜜橘组织培养及植株再生

以南丰蜜橘种子无菌苗的子叶、上胚轴、茎端、下胚轴为材料,在不同激素配比的MS基本培养基上进行外植体筛选和诱导不定芽分化、继代及生根培养,确定了诱导植株再生的最佳外植体和最适条件:(1)诱导不定芽分化最好外植体为上胚轴,不定芽分化率达90%;(2)继代培养中最适激素配比为MS+0.8 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/LD-泛酸钙;(3)生根最适合培养基为MS/2+0.1 mg/L NAA.     

番茄高效再生体系的建立

以砧木1号番茄的子叶和下胚轴为外植体进行离体组织培养,研究了IAA和6-BA及NAA和6-BA不同激素配比对外植体诱导不定芽及生根的影响。结果表明:培养基MS+0.2mg/L IAA+1.0mg/L 6-BA为诱导子叶外植体愈伤组织和不定芽的最适培养基,而MS+0.5mg/L IAA+1.0mg/L 6-BA为诱导下胚轴外植体愈伤组织和不定芽的最适培养基;诱导不定芽生根的最佳培养基为MS+0.1mg/L IAA。     

酸浆的组织培养与植株再生

以酸浆的叶片、叶柄、茎段、子叶、胚轴、胚根为外植体进行离体培养,结果表明:叶柄为酸浆不定芽分化的最佳外植体类型;IAA为诱导叶柄不定芽分化较适合的生长素类型;不定芽分化的最佳培养基为MS 2.0 mg/L 6-BA 0.5 mg/L IAA;丛生芽增殖的最佳培养基为MS 2.0 mg/L 6-BA 0.05 mg/L IAA,增殖系数达5.0;最优的生根培养基为1/2 MS,生根率达91.7%.     

大丽花组织培养及快繁技术研究

目的探讨适合大丽花离体快繁和再生的外植体类型和激素配比.方法以D6大丽花品种为试材,比较不同类型外植体愈伤组织和芽的分化情况,以及不同激素配比对腋芽和叶片愈伤组织诱导和分化的影响.结果顶芽和带腋芽的茎段在MS+6-BA 3 mg/L+NAA 0.1 mg/L培养基上容易形成丛生芽;刚萌动的腋芽在MS+6-BA 1 mg/L+NAA 0.2 mg/L培养基上出芽率达到81.3%,平均芽数为3.8;叶片在MS+6-BA 3 mg/L+NAA 0.1 mg/L的培养基上不定芽的分化率为37.5%,平均芽数为4.2.结论顶芽和带腋芽茎段是进行离体快繁最适合的外植体,适合刚萌动的腋芽生长和分化的激素配比是6-BA 1 mg/L+NAA 0.2 mg/L,适合叶片分化不定芽的激素配比是6-BA 3 mg/L+NAA 0.1 mg/L.     

毛酸浆下胚轴组织培养研究

以毛酸浆下胚轴为外植体,研究不同激素配比对其愈伤组织诱导、分化及不定芽生根的影响.结果表明,愈伤组织诱导最佳培养基为MS+6-BA 3mg/L+NAA 0.2mg/L,愈伤组织分化最佳培养基为MS+6-BA 2mg/+IAA 0.2mg/L,不定芽生根最佳培养基为MS+NAA 0.1mg/L或MS+IBA 0.1mg/L.研究结果为毛酸浆的规模化生产、种质资源的保存和遗传转化提供了理论依据.     

麻疯树根高效再生体系的建立及不定芽起源研究

为了克服现有麻疯树快繁技术的不足,分别以麻疯树胚根和子叶外植体诱导的不定根为外植体诱导不定芽.麻疯树子叶不定根诱导的最佳培养方式为:MS+5 mg/L IBA上培养4天,然后转至MS0培养基上生根.诱导生根率和生根数分别达到93.33%和6.54个/外植体.子叶不定根诱导不定芽的最佳培养基是MS+3.0 mg/L 6-BA+3 mg/L IAA,诱导率达到83.33%.麻疯树胚根的最佳不定芽诱导培养基为MS+0.2 mg/L 6-BA+1.0 mg/L IBA,分化率达67.80%,平均出芽数为3.25个/外植体.对麻疯树胚根再生的各阶段进行了石蜡切片观察,明确了不定芽起源于根的薄壁细胞层.与现有技术相比,本方法具有周期短、再生效率高的特点.     

白术胚轴和胚根直接分化不定芽的再生体系

为了建立白术Atractylodes macrocephala Koidz.通过胚轴、胚根外植体直接分化不定芽的高效离体再生体系,研究了外植体类型、植物生长调节剂种类及质量浓度对不定芽直接分化的影响.研究结果表明,以白术无菌苗的胚轴、胚根为外植体,在分化培养基上可直接诱导不定芽分化,其中胚轴不定芽分化率最高可达91.18%,每个外植体产生芽的数量最高可达13个;诱导不定芽分化的最佳培养基为MS(Murashige and Skoog)+ TDZ(thidiazuron)1.5 mg/L+ NAA(naphthylacetic acid)0.2 mg/L.将分化的不定芽培养于附加IBA(indol 3-butiric acid)0.5 mg/L的1/2 MS培养基中诱导生根,生根率达66.66%以上.本研究为药用植物白术的工厂化育苗和脱病毒以及利用植物基因工程技术进行品质改良提供了有效的再生途径.     

虎斑海棠的组织培养和快速繁殖

实验以虎斑海棠叶片为外植体,探讨了生长调节物质对外植体诱导分化、生根的影响.结果表明:MS (6-BA)1 mg/L NAA0.2 mg/L和MS (6-BA)2 mg/L NAA0.2 mg/L是虎斑海棠离体叶片不定芽产生的适宜培养基,在MS NAA0.5 mg/L上的生根率达90%,长成完整的再生植株,达到快速繁殖的目的.     

“三月花”黄花菜的组织培养研究

采用单因素和正交实验方法对"三月花"黄花菜的组织培养技术进行了探索.结果显示:幼叶用0.1%升汞灭菌12 min时效果最佳,外植体污染率、死亡率均为0, 14 d后外植体启动率为62.5%.最佳愈伤诱导培养基为MS+2 mg/L 2,4-D+0.1 mg/L 6-BA,外植体出愈率为86.67%;愈伤组织最适分化培养基为MS+2 mg/L 6-BA+0.5 mg/L IBA,愈伤组织分化率达到80%,平均每块愈伤组织不定芽个数为1.72.最佳生根培养基为1/2 MS+0.5 mg/L NAA,生根率达到93.33%,平均每个芽苗生根数为5.83条,试管苗炼苗移栽后,成活率达95%.利用该组织培养技术,幼叶外植体通过愈伤组织途径获得"三月花"黄花菜再生苗.     

旱柳体外培养与直接分化再生系统的建立

给出了旱柳体外培养的方法:9月份至12月份采集1~2 a生枝条上带腋芽的茎段,经过筛选确定MS+0.5 mg/L 6-BA+0.1 mg/L NAA为最佳诱导培养基,平均每个芽点产生的不定芽数量为3.5个,20 d后苗高为1.8 cm;不定芽在MS+0.1 mg/L 6-BA+0.2 mg/L NAA培养基中继代增殖及生长较好,为适宜的增殖培养基;在形成的不定芽中有些不定芽起源于单株苗的切口基部,因此,初步建立了旱柳的直接分化再生系统,可为旱柳的遗传转化等研究奠定基础.经过壮苗处理的幼苗在MS+0.2 mg/L NAA培养基上的生根率达100%,且生根量多,幼苗生长健壮.生根苗移栽至V(草炭土)V(河沙)为3 1的混合基质中,60 d后成活率为90%左右.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that