黑眉锦蛇和乌梢蛇几种组织中乳酸脱氢酶同工酶的比较研究

本文采用聚丙烯酰胺凝胶电(冫永)方法,研究了黑眉锦蛇和乌梢蛇血清、心肌和肝脏的乳酸脱氢酶同工酶。结果表明:该二种蛇的血清仅具有LDH_1一种同工酶;心肌有三种LDH同工酶,它们以各自的特异性比例而存在,以B亚基占优势为特征。在肝脏中,两种蛇都具有四种LDH同工酶主带,乌梢蛇还有一条附属于LDH_2的亚带而不同于黑眉锦蛇。根据实验结果,本文初步探讨了该二种蛇的分类地位,同时还对乳酸脱氢酶(LDH)同工酶与遗传、生理、生化、生态的关系作了讨论。     

蟾蜍组织LDH同工酶的比较研究

本文采用聚丙烯酰胺盘电泳法,对中华大蟾蜍(Bufo gargarizans)在冬眠期与活动期的肝、骨骼肌、心肌和脑组织进行了乳酸脱氢酶(LDH)同工酶的比较生化研究。结果表明,冬眠期蟾蜍与活动期蟾蜍的LDH同工酶是不同的,向一种组织LDH同工酶谱显示的区带数,在冬眠期较活动期多,相对活力也不同,心肌的LDH_5区带,在冬眠期还出现了两条亚带。     

陕西蝮蛇的血清LDH同工酶研究

用聚丙烯酰胺凝胶电泳法(PAGE)研究了陕西蝮蛇三亚种的血清乳酸脱氢酶(LDH)同工酶。结果表明,蝮蛇短尾亚种仅有5条带,中介亚种和秦岭亚种各显6条带,其中,中介亚种LDH_5分化出一条亚带,秦岭亚种LDH_1与LDH_2间出现一染色稍深的同工酶带。三者间酶带的含量与分布亦有不同。可见,三者间在糖代谢的方式上存在着一定差异。这对应用LDH同工酶分析法解决蝮蛇分类问题提供了新的参考。     

西藏山溪鲵乳酸脱氢酶同工酶（LDH）的初步研究

采用聚丙烯酰铵胶电泳（PAGE）对西藏山溪鲵14种组织的乳酸脱氢酶（LDH）同工酶谱系进行了研究，酶谱带在各种组织中的均表现为5带型，14种组织器官的LDH同工酶可分为三种类型：第一种为LDH1及H亚基占优势的，包括心肌，生殖腺，第二种为H基与M亚基7含量近乎相等的，包括皮肤，脾，肾等，第三种为LDH5及M亚基占优势，包括小肠，胃，肝，肺，肌肉，血清，眼 ，脑等，并将对西藏山溪鲵组织LDH同工酶种的特异性及组织特异性成因进行了初步讨论。     

内江猪、伍隍猪生化遗传及细胞遗传的研究:Ⅰ血清乳酸脱氢酶同工酶的分离测定

采用聚丙烯酰胺凝胶盘状电泳法,在完全相同条件下,分别测定了三月龄和六月龄的内江猪、伍隍猪的血清乳酸脱氢酶(LDH)同工酶,并与相应月龄的体尺、体重等进行分析。结果表明:内江猪和伍隍猪血清LDH同工酶表现出五条正常酶带。由正极到负板依次为LDH_1,LDH_2,LDH_3,LDH_4,LDH_5。三月龄时,内江猪和伍隍猪血清LDH同工酶活力均值(％)的顺序是LDH_1>LDH_5>LDH_3>LDH_4>LDH_2,组间差异不显著(P>0.05)。六月龄时,两组LDH同工酶活力均值(％)顺序为LDH_1>LDH_5>LDH_4>LDH_3>LDH_3,组间差异也不显著(P>0.05)。内江猪的LDH同工酶三月龄与六月龄相比无显著差异(P>0.05)。而伍隍猪的LDH_2在三月龄时极显著的高于六月龄(P<0.01),其余差异不显著。血清LDH同工酶与体尺体重等的关系,在内江猪与伍隍猪之间和三月龄与六月龄之间均未发现什么规律性。     

树(鼠句)(Tupaia belangeri chinensis)消化器官和性腺LDH同工酶的电泳分析

采用0.5%琼脂糖凝胶电泳方法对云南树鼩的肝脏、胃、小肠、胰腺、睾丸和卵巢组织的乳酸脱氢酶同工酶进行电泳分析.结果表明,在6种不同组中,乳酸脱氢酶均呈现为5种不同分子形式的同工酶.其LDH_4至LDH_1同工酶随电泳迁移率的增大依次趋于阳极端,LDH_5同工酶则趋于阴极端.按分光光度比色定量法对各同工酶组分进行洗脱定量,分别测得6种组织中5种同工酶组分的相对百分含量.通过酶谱分析,提示在6种不同组织中由于A、B两种基因表达活性的差异,其酶谱特征也各不相同.文中对树鼩6种组织的LDH同工酶的H和M亚基百分率及H/M亚基比率进行了统计分析,提示这与各类组织的生物学功能及代谢类型相关.     

北京鸭LDH_1提纯及其电泳分析

本文采用琼脂糖4B交联不溶性丙酮酸类似物(草酸盐衍生物)的亲和层析法及DEAE-Sephadex A50层析柱两种方法对北京鸭心肌LDH_1进行分离纯化比较。 琼脂糖4B交联丙酮酸类似物层析柱具有高的亲和特性。比较经过亲和前后的样品通过DEAE-Sephadex A50柱二次纯化后,发现LDH同工酶在聚丙烯酰胺凝胶电泳图谱上出现二条具LDH酶活性的蛋白条带。其中LDH_1的量远远超过LDH_2的量。 在等电聚焦电泳的实验中,发现多肽现象电泳图谱表明LDH_1有10条亚带。LDH_2有9条亚带。     

金线蛙指名亚种、金线蛙福建亚种和黑斑蛙的脑、心、肝和肾组织的乳酸脱氢酶同工酶的分析比较

本文比较了不同地区产的金线蛙指名亚种(北京产的、杭州产的)、金线蛙福建亚科和黑斑蛙的脑、心、肝、肾组织中的乳酸脱氢酶(LDH)同工酶的酶谱.金线蛙指名亚种和金线蛙福建亚种 LHD 同工酶迁移率较一致,但是,它们的酶谱从 LDH_1—LDH_5各组分含量的多寡有区别。因此,我们认为把它们作为两个不同亚种处理较为恰当.其次,不同地区(北京相杭州)产的金线蛙指名亚种 LDH 同工酶酶谱虽然相似,但是,北京产的金线蛙指名亚种和杭州产的金线蛙指名亚种在脑、肝组织中 LDH 同工酶的组份,还有一些差异.电泳图谱表现出黑斑蛙 LDH 同工酶 LDH_1、LDH_2、LDH_3、LDH_4与金线蛙的相比,都明显地具有较小的迁移率。     

花背蟾蜍冬眠期和繁殖期几种器官组织中酶的研究

本文利用聚丙烯酰胺凝胶盘状电泳,测定了花背蟾蜍冬眠期和繁殖期6种器官组织中乳酸脱氢酶(LDH)同工酶的相对活性.结果表明,6种器官组织中 LDH 同工酶的活性表现明显的季节和性别差异.在肝脏的 LDH 同工酶酶谱中还出现亚带和第6条酶带.     

人体乳酸脱氢酶(LDH)同工酶系统研究(二)——结、直肠癌患者组织LDH同工酶研究

利用紫外分光光度法,略加修正的聚丙烯酰胺凝胶电泳、染色方法和薄层层析扫描技术对北京区总医院提供的九例结、直肠癌(其中,结肠癌三例、直肠癌六例)癌组织及癌旁正常组织LDH总活性及其各种同工酶相对百分活性进行了定量测定。初步实验结果表明:结、直肠癌LDH总活性显著升高。LDH_2和LDH_2相对百分活性均略有减少,LDH_5值成倍增长。直肠癌LDH_2值减少和LDH_4值增加均非常显著。此外,病理鉴别“远端”正常,近癌和癌组织数值对比研究表明:LDH_3值最高,呈现“远端”正常<近癌>癌。LDH_1和LDH_2则呈现“远端”正常>近癌>癌,而LDH_4和LDH_5则另是一样,呈现“远端”正常<近癌<<癌组织模式。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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