共查询到20条相似文献,搜索用时 125 毫秒
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GAL4 activates transcription in Drosophila 总被引:32,自引:0,他引:32
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Yeast activators stimulate plant gene expression 总被引:25,自引:0,他引:25
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将编码人c-myc羧基端bHLH/LZ结构域的92个氨基酸的cDNA片段(c-myc-c92)对框插入pGEX-2T原核表达载体中,使之与谷胱甘肽转移酶(GST)编码基因融合,并将重质粒导入大肠杆菌BL21(DE3)中,由IPTG诱导融合基因高效表达,并用Glutathione Sepharose4B亲和柱纯化融合蛋白GST-c-Myc-c92,凝胶阻滞(EMSA)分析显示该纯化蛋白能与CACGTG序列特异结合,并且只有高浓度的GST-c-Myc-c92才能与探针结合,结果表明在体上情况下高浓度c-Myc羟基端能自身二聚体化,此发现丰富了Myc-Max-Mad的调控网络,并为进一步研究c-myc的功能和调控提供了一定的线索。 相似文献
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Cloning and domain structure of the mammalian splicing factor U2AF. 总被引:60,自引:0,他引:60
A complementary DNA clone encoding the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA. 相似文献
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The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain 总被引:54,自引:0,他引:54
L P Freedman B F Luisi Z R Korszun R Basavappa P B Sigler K R Yamamoto 《Nature》1988,334(6182):543-546
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The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis 总被引:19,自引:0,他引:19
Fas is a cell-surface receptor molecule that relays apoptotic (cell death) signals into cells. When Fas is activated by binding of its ligand, the proteolytic protein caspase-8 is recruited to a signalling complex known as DISC by binding to a Fas-associated adapter protein. A large new protein, FLASH, has now been identified by cloning of its complementary DNA. This protein contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. Stimulated Fas binds FLASH, so FLASH is probably a component of the DISC signalling complex. Transient expression of FLASH activates caspase-8, whereas overexpression of a truncated form of FLASH containing only one of its DRD or CED-4-like domains does not allow activation of caspase-8 and Fas-mediated apoptosis to occur. Overexpression of full-length FLASH blocks the anti-apoptotic effect of the adenovirus protein E1B19K. FLASH is therefore necessary for the activation of caspase-8 in Fas-mediated apoptosis. 相似文献
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通过筛选人18周胎脑cDNA文库,得到一条编码332AA的全长新基因,生物信息学研究表明,该蛋白质序列有2个C2H2型锌指结构,其中1个锌指结构有RNA-binding蛋白特异锌指的特征,虽然同源比较发现与多种蛋白质精氨酸N端转甲基酶(protein arginine N-methyltransferase) 有一定的同源性,但新锌指蛋白不含转甲基酶的活性功能区域,属功能未知的基因,利用芯片研究功能未知基因的表达是一种较好的手段,通过代谢增强剂PMA(phorbol myristae acetate)刺激培养的血管内皮细胞,观察细胞受激活后新锌指蛋白基因的表达变化,结果表明新基因表达量提高了11倍以上,证实新基因属内皮细胞的极早期应答基因(Immediate early response gene,ERG)。 相似文献
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Negative effect of the transcriptional activator GAL4 总被引:89,自引:0,他引:89
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Shi Y Sawada J Sui G Affar el B Whetstine JR Lan F Ogawa H Luke MP Nakatani Y Shi Y 《Nature》2003,422(6933):735-738