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1.
目的探究金丝桃属基因组DNA的提取方法及用RAPD分析的技术体系.方法采用改良的低pH值高盐法获得高质量的金丝桃属植物总DNA,从80个10-mer随机引物中筛选出12个,对4种金丝桃属植物的基因组DNA进行RAPD分析.结果共产生了330条DNA片段,其中218条谱带具有遗传多态性,约占66.1%.结论 RAPD分析揭示4种金丝桃属植物之间的DNA指纹存在明显的种间差异,能为金丝桃属植物的分类鉴定提供遗传学依据.  相似文献   

2.
4种木麻黄亲缘关系的RAPD分析   总被引:15,自引:0,他引:15  
应用RAPD枝术对普通木麻黄(Casuarina equiseti folia),粗枝木麻黄(C.gluca),细枝木麻黄(C.cunninghamaina)和滨海木麻黄(Allocasuarina littoralis)的亲缘关系进行分析,从40个引物中筛选出18个重复性好、稳定性高的有效引物.18个有效引物共扩增出690条DNA带,其中多态性条带318条,占总扩增条带的46.09%.对扩增出来的690条DNA带进行聚类分析,结果表明:木麻黄属(Casuarina)的普通木麻黄、粗枝木麻黄和细枝木麻黄的平均遗传距离为0.30,其中细枝木麻黄和粗枝木麻黄的遗传距离特别小,为0.19;而异果木麻黄属(Allocasuarina)的滨海木麻黄与其他3种木麻黄的平均遗传距离为0.65.RAPD聚类分析结果表明,4种木麻黄之间的亲缘关系与血清学和化学分类学的结果一致,说明RAPD分子标记技术适用于木麻黄植物的遗传多样性研究.  相似文献   

3.
用随机扩增多态性DNA(RAPD)技术对刺槐属(Robina)内5种植物进行基因组DNA多态性分析.共选用22个10 bp随机引物扩增出212个DNA片段,进行种间遗传关系分析.片段大小在100~2 200 bp之间,其中多态性谱带为183条,多态率为86.3%;利用UPGMA进行遗传距离计算的结果表明,刺槐、毛刺槐和香花槐分属刺槐属的三个种,红花刺槐和龙槐是刺槐的变型.首次在DNA水平上证明香花槐为刺槐属的一个独立种,且与毛刺槐的亲缘关系较近.为刺槐属植物亲缘关系和系统分类建立了新的分子学证据.  相似文献   

4.
本研究利用RAPD和ISSR两种分子标记技术.对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究.从30个RAPD引物当中.筛选出11个具有多态性的引物.共扩增出多态性带224条.多态性条带比率为41.18%.从12个ISSR引物中筛选出5个具多态性的ISSR引物.共扩增出多态性带121条.多态性条带比率为50.21%.对RAPD和ISSR两种标记分别运用Nei指数法.计算出10个大蒜品种的平均遗传距离为0.28和0.32.根据这两种标记的结果.采用UPGMA进行聚类分析,得到与生物学分类地位基本一致的结果.结果表明,在实验稳定性上.ISSR优于RAPD.且ISSR比RAPD能检测到更多的遗传变异.RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究.  相似文献   

5.
十一种重楼属植物的RAPD分析   总被引:6,自引:3,他引:6  
作者利用随机扩增多态性DNA(RAPD)技术,对重楼属植物11个种的遗传多样性进行了分析.12个引物共扩增出86条带,其中有82条(95.3%)表现出多态性,说明重楼属植物有很高的遗传多样性.用UPGMA法则构建分子系统树,并把11个种划分为6组.  相似文献   

6.
6种瓢虫的RAPD分析及在分类上应用的研究   总被引:11,自引:0,他引:11  
利用随机引物分别对6种瓢虫的DNA进行了聚合酶链式反应(RAPD-PCR)的实验。结果显示,用相同引物扩增不同种瓢虫的基因组DNA,扩增产物多态性DNA片段的数目是不同的;两种不同引物的扩增产物中,各自呈现出种、属的特异性片段,其可用于种、属的鉴别;聚类图显示了种、属之间亲缘关系的远近程度,并与形态学分类结果相一致。实验分析还发现:七星瓢虫不同个体间多态性DNA特有带较多,遗传较稳定;龟纹瓢虫与之相比,不同个体间多态性DNA的特有带较少,变异程度较大。  相似文献   

7.
以植物组织培养技术建立的9种晋产北柴胡(Bupleurum chinense DC.)试管植株为材料,用CTAB法提取北柴胡试管植株叶片的总DNA,以随机引物进行非特异性扩增,计算了遗传相似系数与遗传距离,并进行了聚类分析.从20条随机引物中挑选出3条扩增条带清晰、重复性好的引物OPC-8、OPD-8和OPE-10,共检测出22条DNA谱带,其中多态性条带18条(占82%).结果表明,9种北柴胡试管植株间均存在不同的遗传差异,根据绘制的聚类图可将9种不同类型的北柴胡聚类为4组.说明RAPD多态性分析从分子水平上一定程度反映出不同类型晋产北柴胡之间的遗传多态性.  相似文献   

8.
采用随机扩增多态性DNA(RAPD)技术 ,对螽总科 1 0种昆虫的RAPD带型变异进行了分析 .带型显示出属、种间多态性明显 ,能够明显区分 .利用NU取得遗传距离与遗传一致性矩阵 ,并用UPGMA聚类法进行分析 ,取得了系统树  相似文献   

9.
大蒜种质资源遗传多样性的分子标记研究   总被引:1,自引:0,他引:1  
本研究利用RAPD和ISSR两种分子标记技术,对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究.从30个RAPD引物当中,筛选出11个具有多态性的引物,共扩增出多态性带224条,多态性条带比率为41.18%,从12个ISSR引物中筛选出5个具多态性的ISSR引物,共扩增出多态性带121条,多态性条带比率为50.21%.对RAPD和ISSR两种标记分别运用Nei指数法,计算出10个大蒜品种的平均遗传距离为0.28和0.32.根据这两种标记的结果,采用UPGMA进行聚类分析,得到与生物学分类地位基本一致的结果.结果表明,在实验稳定性上,ISSR优于RAPD,且ISSR比RAPD能检测到更多的遗传变异,RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究.  相似文献   

10.
果蝇突变体的RAPD分析   总被引:1,自引:0,他引:1  
采用RAPD技术对遗传学模式生物——果蝇的野生型及7种突变体的遗传差异进行比较研究.结果表明,所选用的37种RAPD引物中,有25种引物扩增谱带清晰,扩增总片断284条,单个引物的扩增片段数为6~15条,平均为11.36条.不同果蝇品种间的遗传差异较小,遗传距离(D)为0.0334~0.2089.根据D值,由UPGMA聚类分析绘制了它们的分子进化树。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

14.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

15.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

17.
Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.  相似文献   

18.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

19.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

20.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

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