共查询到20条相似文献,搜索用时 15 毫秒
1.
Mi Ruifa Shi Xiangqun Liu Shuhong Zhou Changman Wang Jiazheng Fan Ming 《科学通报(英文版)》1999,44(3):225-225
The techniquein situ hybridization was used to measure the levels of light (NF-L), medium (NF-M) and heavy (NF-H) neurofilament protein subunits
mRNA in L4-6 spinal motoneurons in adult rat during regeneration following a unilateral crush of the sciatic nerve. It was found that
the hybridization signals of each neurofilament subunit rnRNA were dramatically decreased in spinal motoneurons postaxotomy
by light microscopy. The hybridization signals of NF-L and NF-M mRNA were located in cytoplasm of neurons, whereas NF-H mRNA
was found in both nucleus and cytoplasm of neurons. Image analysis showed that the encoding levels of mRNA for each of neurofilament
subunit mRNA reduced on the 3rd d and returned to control levels on the 28th d following the lesion. The relative levels of
mRNA coding for each neurofilament subunit were significantly different. The lowest level of NF-L mRNA was observed at 5 d
postaxotomy, and that of NF-M, NF-H mRNA on the 7th and 10th d after injury. Moreover, the levels of HF-M and NF-H mRNA were
reduced much lower and lasted much longer than that of NF-L mRNA. The observations suggest that there were different mechanisms
for the regulation of neurofilament subunit genes expression. The reduced neurofilament gene expression may be due to a response
to axonal injury and advance the restructure of axonal architecture. 相似文献
2.
3.
Mapping of low or single-copy sequences on plant chromosomes has proven difficult because of very low frequency of signal
detection. Rice BAC library is being used widely in rice genome research due to its distinctive advantages over other library
systems. In this study, two biotin-labeled rice BAC clones closely linked to a rice blast resistance, green leafhopper resistance
and tungro spherical virus resistance gene,Pi-5(t), Glh, RTSV, werein situ hybridized to rice chromosomes. They were located on the long arm and short arm of chromosome 4 with FL value of 40% and
100% respectively. The frequency of signal detection reached 46.8% and 59.2%. The signal location were consistent with the
selective marker on rice saturated molecular map. The results demonstrated the advantages to locate BAC clones to chromosomes
byin situ hybridization and will facilitate the rice low or single-copy gene location by using the BAC library.
Supported by the National Natural Science Foundation of China and the Doctorate Vesting Point Foundation of the Education
Department of the People's Republic of China
Yan Huimin: born in 1964, Lecturer 相似文献
4.
Yiwen Li Shunxue Tang Tiehan Zhao Hui Liang Hongjie Li Shuang’e Jia Zhensheng Li Xu Jia 《科学通报(英文版)》1999,44(14):1294-1294
The translocation frequency up to 3.7% has been obtained by crossing aTriticum-Thinopyrum substitution with aTriticum-Haynaldia substitution. The pattern of translocations includes not only wheat-alien Robertson translocations but also inserting translocations
involvingThinopyrum chromatin andHaynaldia chromatin. The result indicates that it may be a new method of producing translocations to make two different wheat-alien
substitution cross with each other. 相似文献
5.
6.
Zhang Xiyuan Jiang Haibo Ma Qi Yang Jianqi Li Lijia Liu Ting Yan Ju 《武汉大学学报:自然科学英文版》1996,1(1):119-124
Using the BrdU antibody technique followed by an immuno-chemical staining (BAT), the amplification of DNA fragments specific
to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products
orin situ hybridization with PCR products on slides, the amplified target DNA fragments of specimen were visualized by BAT under the
microscope. The availability of BAT and differences in the sensitivity and efficiency between BAT and dig-11-dUTP labeling
in cellin situ PCR were discussed.
Zhang Xiyuan: born in Oct. 1935, Professor, Current research interest in Cellular and Molecular Biology
Supported by the National Natural Science Foundation of China 相似文献
7.
The cellular localization and specific expression of TR3 mRNA in rat testis were investigated by the method of immuno- histochemistry andin situ hybridization. It was demonstrated that orphan receptor TR3 was expressed in a significant amount in rat testis and TR3 protein
and mRNA were specifically localized in germ cells, suggesting that TR3 may have an important function in regulating rat germ
cell development. 相似文献
8.
The steroidogenic acute regulatory protein (StAR) is the key regulatory protein of steroidogenesis.De novo synthesis of StAR protein is required for intramitochondrial translocation of cholesterol to the cytochrome P450 side chain
cleavage enzyme which is located on the matrix side of the inner mitochondrial membrane. This is the rate-limiting step of
steroid biosynthesis. Usingin situ hybridization and immunohistochemistry we studied StAR expression in various stages of the corpora luteal and its regulation
by interferon-gamma (IFNγ) in the adult pseudopregnant rat. The results indicated that expression of StAR in the corpora luteal
was correlated with progesteron production and IFNγ was capable of inhibiting its expression. 相似文献
9.
Neural stem cell transplantation in the repair of spinal cord injury 总被引:17,自引:0,他引:17
Neural stem cells are a pronising candidate for neural transplantation aimed at neural cell replacement and repair of the damaged host central nervous system (CNS). Recent studies using neural stem cells have shown that implanted neural stem cells can effectively incorporate into the damaged CNS and differentiate into neurons, astrocytes, and oligodendrocytes. The recent explosion in the field of neural stem cell research has provided insight into the inductive factors influencing neural stem cell differentiation and may yield potential therapies for several neurological disorders, including spinal cord injury. In this review, we summarize recent studies involving neural stem cell biology in both rodents and humans. We also discuss unique advantages and possible mechanisms of using neural stem cell trans plantation in the repair of spinal cord injury. 相似文献
10.
Expression and regulation of orphan receptor TR2 mRNA in germ cells of cryptorchid testis in rat and rhesus monkey 总被引:3,自引:0,他引:3
Expression and cellular localization of orphan receptor TR2 mRNA in relation to germ cell apoptosis in cryptorchid testes
of rat and rhesus monkey have been studied by usingin situ hybridization andin situ 3′-end labeling of DNA fragments (TUNEL). The results show that: (i) TR2 mRNA is specifically expressed in the germ cells,
mainly in the spermatocytes, round and elongated spermatids. The expression level of TR2 mRNA varies with the seminiferous
cycle, (ii) In the rat cryptorchid testes on days 3 and 5 after the surgery, the germ cells began to undergo apoptosis with
no evident decrease in TR2 mRNA level. On day 7.5, however, most germ cells underwent apoptosis, while the expression level
of TR2 mRNA declined markedly, and TR2 mRNA was rarely expressed on day 10 thereafter, (iii) On days 15 and 20 of the cryptorchid
testes of rhesus monkey, TR2 mRNA was only expressed in a few of primary spermatocytes and the mRNA was almost undetectable
on days 30, 45, 60. These results suggest that TR2 mRNA probably plays an important role in spermatogenesis and germ cell
apoptosis. 相似文献
11.
Antral follicles are present in the ovaries throughout gestation. These follicles are “physically immature” and cannot ovulate
under the induction of LH/hCG. The purpose of the present study is to examine whether follicles in pregnant rat ovaries are
capable of steroidogenesis. StAR is believed to be the key regulator of steroid hormone biosynthesis. Antisense StAR probe
and anti-StAR rabbit serum have been used to detect the StAR expression in ovarian follicles at various stages of pregnant
rats. The results indicate that theca-interstitial cells and the membrane granulosa cells at the stages from the estrous or
pre-estrous in the normal cycling rat ovary express StAR mRNA and its protein, whereas neither granulosa cells nor the theca
cells in pregnant ovary throughout gestation express StAR. These results indicate that the pregnant follicles throughout gestation
are incapable of steroidogenesis. 相似文献
12.
A male-sterileT. aestivum-Ag. intermedium partial amphiploid with cytoplasm ofT. timopheevii as a female parent was crossed to common wheat. The hybrid was backcrossed to the male parent several times continually and
setf-crossed at last. Two stable lines with common wheat phenotype, H96269-2 and H96278, have been obtained. The chromosome
numbers of the two lines are all2n = 42 in somatic cetls. By inoculation test, the two lines show a high levet of resistance to yetlow rust. Through genomicin situ hybridization (GISH) withAg. intermedium total genomic DNA as a probe, it is demonstrated that the two stable lines are all small segmental translocation lines, and
the translocated chromosome segments fromAg. intermedium are located on the short arm terminals of wheat chromosomes. Genetics analysis suggests that the yetlow rust resistance gene(s)
are probably located on the translocated chromosome segments ofAg. intermedium. 相似文献
13.
Fluorescence in situ Hybridization (FISH) was used to investigate whether the chromosome of the fetus prenatally diagnosed as pyelectasis was normal or not. Amniotic fluid was taken from the pregnant woman whose fetus was detected with pyelectasia by prenatal examination. The chromosome of the amniotic fluid cell without culture was examined with FISH. The result shows that compared with the traditional amniotic fluid cell culture, FISH has the advantages of more rapid, higher sensitivity and specificity, and was 10-12 days earlier to complete the diagnosing than the traditional method. The fetuses detected chromosomal abnormality in each groups were induced during the middle and late trimester, while those fetuses with normal chromosome continued pregnancy, the rate of spontaneous disappearance of pyelectasia decreased as the severity of pyelectasia increased. FISH can satisfy the urgent need in the clinical prenatal diagnosis due to its rapidity to determine whether fetus with pyelectasia was accompanied with chromosomal. 相似文献
14.
Wheat-related species Th. intermedium was used to cross with common wheat Yannong 15. In the self progenies of the hybrid, two addition lines, Ⅱ-1-7-1 and Ⅱ-3-3-2, stable in cytology, were developed by cytology and powdery mildew resistance identification. Their chromosome number were 2n = 44 and formed 22 bivalents at PMC MI. In F1 of the two addition lines crossing with Yannong 15, there appeared about one univalent at PMC MI, respectively. Resistance identification in greenhouse and field using the No. 15 and mixed strains of E. gramnis f. sp. tritici showed that they were immune to powdery mildew. Chromosome number and resistance identification using the F2 single plants of the addition line crossing with Yannong 15 indicated that the resistant gene was located on the alien chromosomes. In situ hybridization using St and E genomic DNA as probe showed that the added chromosome in the two addition lines probably came from the E genome of Th. intermedium, which indicated that a pair of E genome chromosomes carried a new resistant gene to powdery mildew. 相似文献
15.
The catalyst [(2-ArN=C(Me))2C5H3N]FeCl2 (Ar = 2-C6H4(i-Pr)) with methylaluminoxane (MAO) as cocatalyst was intercalated between layers of montmorillonite (MMT) for ethylene oligomerization.
Metallocene catalyst Me2Si(Ind)2ZrCl2 and MAO was then added to form a dual functional catalytic system. A PE/MMT nanocomposite was prepared by copolymerization
of ethylene with α-olefins produced in situ from ethylene over the dual functional catalytic system. The catalytic system was of high polymeric activity. The resultant
PE/MMT nanocomposites were stable and got increases in tensile strength and temperature of maximum weight loss (Tonset). 相似文献
16.
Byin situ hybridization, the localization of orphan receptor TR3 mRNA has been observed in early developmental follicles. TR3 mRNA
is first expressed in the ovarian interstitial cells on day 2 after birth, and then in granulosa cells (GC) in primary follicles
on day 4. The expression level of TR3 mRNA in GC increases following the follicular development. Its higher expression can
be observed in the outer layer of GC and inner layer of theca cells (TC) on day 6, where the cells present active proliferation
and differentiation. The expression of TR3 is in an increasing manner until the large antral follicles on day 30. The mRNA
is only expressed in the healthy, but not atretic follicles in adult rat ovaries. Injection of epidermal growth factor (EGF)
has dramatically enhanced its expression in the early stage of developmental follicles. It is therefore suggested that TR3
may play a role in regulating growth and differentiation of ovarian somatic cells in the early stage, and its expression is
regulated by EGF. 相似文献
17.
根据2019年夏季长江口及邻近海域17个站位的大中型浮游生物与水质参数分析,研究了大中型浮游生物的空间分布特征及其主要影响因素。在长江口咸淡水混合影响下,长江口-东海陆架断面浮游生物物种数及生物多样性指数均随盐度增加而增大,且淡水及低盐度站位与高盐度站位的优势物种存在显著差异。长江口-东海陆架关键断面13个站位的大中型浮游生物密度及生物量与叶绿素a无显著相关性,与营养盐含量呈显著负相关,表明上行效应并非控制该断面浮游生物分布特征的主要因素。在咸淡水混合所致多因素变化的复杂影响下,该断面大中型浮游生物分布的主要影响因素呈现出盐度相关的区域性差异。首次利用浮游生物在线成像和分类设备(CPICS)在多个高盐度站位开展原位观测,获得了多种大中型浮游生物的原位影像,可对夜光藻、双生水母等大型浮游动物鉴定至种,对桡足类鉴定至亚纲。CPICS垂直布放原位观测与野外垂直拖网采样分析结果所反映的物种数变化趋势具有一致性。 相似文献
18.
Yixun Liu Zhaoyuan Hu Qiang Feng Hongjuan Gao Kui Liu Colin D. Ockleford 《科学通报(英文版)》2000,45(16):1484-1489
Human placental tissues from the first and second trimesters of gestation have been investigated using riboprobein situ hybridisation of mRNA sequences coding for membrane type metalloproteinase (MT-1-MMP) and tissue inhibitors of metalloproteinase-1
(TIMP-1). Results show that (i) both mRNAs express at a relatively high level in the chorion laeve trophoblast cells and the
adjacent decidual cells of fetal membrane; (ii) the most abundant expression of the two mRNAs was found in the extravillous
trophoblast between Rohrs and Nitabuch striae of basal plate, trophoblast shell and gland cells of the decidua; (iii) isolated
or small groups of cytotrophoblast cells in the chorionic villi and in the cells lining arterioles in decidua and stem villi
also expressed both MT-1-MMP and TIMP-1 at defferent extents. The data suggest that the coordinated expression of the MT-MMP
and its inhibitor TIMP in defferent cells of the placental tissue may play an essential role in trophoblast invasion and angiogenesis
related to placentation in the first two trimesters of gestation. They may also have an ability to effect separation of fetal
from material tissue at a favorable junctional site during parturition. 相似文献
19.
Fertile organs andin situ spores of a matoniaceous fern from the Lower Jurassic of West Hubei 总被引:1,自引:0,他引:1
Fossil matoniaceous plants represented byPhlebopteris have been formerly described and illustrated from the Mesozoic of China mainly based on vegetative leaves, and no information
is provided on the fine structure of fertile organs. Recent reinvestigation of the Hsiangchi Flora from the Lower Jurassic
of West Hubei, China, obtained a rich collection of well preservedPhlebopteris specimens (both sterile and fertile). Studies onPhlebopteris polypodioides Brongniart using LM and SEM reveal not only the structures of sori and sporangia, but also the details ofin situ spores. Comparisons have been made between thein situ spores and other related fossil and extant matoniaceous spores, as well as the dispersed genera. 相似文献
20.
根据催化氧化原理,利用4A分子筛为载体,次氯酸钠(NaClO)为氧化剂,通过硫酸锰(MnSO4)在其表面的原位氧化来制备对环境无害的锰氧化物(MnOx)改性的分子筛(MnOx@MS)材料.通过场发射扫描电镜(FE-SEM)和X射线衍射(XRD)对材料进行了表征.XRD分析表明:Mn元素是以MnOx形式存在,在pH为7.2时,反应得到的MnOx中Mn离子的价态最高.测试表明:Mn离子的价态越高对含酚废水的处理效果就越好.采用在最优条件下得到的MnOx@MS材料作为催化剂,对过氧化氢(H2O2)氧化降解含酚废水进行研究,考察了不同体系、温度、pH值和H2O2用量对苯酚降解的影响.结果表明:在优化的降解条件下,120 min内,未改性分子筛对苯酚的去除率仅为35%,而MnOx@MS改性材料对苯酚的去除率达到82%,说明通过MnOx改性后的分子筛,对苯酚的氧化降解能力大大提高. 相似文献