小鼠腹腔巨噬细胞诱生性一氧化氮的诱导合成

通过体外分离培养和体内注射ＴＦＮ－γ，研究了巨噬细胞内一氧化氮的诱导合成，体外２条件下，ＩＦＮ－γ能够诱导小鼠腹腔巨噬细胞合成一氧化氮，与对照组相比有显著差异。巨噬细胞内一氧化氮的诱导合成水平同ＩＦＮ－γ的剂量有关。ＴＮＦ－α能够显著增强ＩＦＮ－γ的诱导合成ＮＯ艇，ＩＦＮ－γ在体内可以增强巨噬细胞的一氧化氮合成能力。研究表明，，诱导巨噬细胞合成一氧化氮是一种非特异性免疫反应，ＩＦＮ－γ诱导免疫细胞     

扶桑花石油醚提取物HR-1对体外培养的人早期胎盘绒毛组织分泌功能的影响(1)

扶桑（ＨｉｂｉｓｃｕｓｒｏｓａｓｉｎｅｎｓｉｓＬ．）花石油醚提取物ＨＲ－１对体外培养的人早期胎盘绒毛组织分泌功能的影响．实验结果表明：ＨＲ－１对人早期胎盘绒毛组织ＨＣＧ和孕酮的分泌、对绒毛滋养层细胞的生长均有较强的抑制作用     

凝血因子IX的体外检测方法研究

凝血因子ＩＸ的检测是血友病Ｂ基因治疗研究中的重要工作，在实验室原有基础上，发现和完善了凝血因子ＩＸ在蛋白质水平上的检测系统，为基因治疗血友病Ｂ提供了更为直观可靠的依据。首先，建立了以鼠抗人ＦＩＸ单克隆抗体Ａ－７为一抗的检测活性ＦＩＸ蛋白量的ＥＬＩＳＡ法，为检测活性ＦＩＸ提供了快速简便的方法。其次，实现了用Ｗｅｓｔｅｒｎｂｌｏｔ法检测转染细胞培养液上清中ＦＩＸ，确证了体外培养的转有人ＦＩＸｃＤＮＡ细     

扶桑花石油醚提取物HR—1对体外培养的人早期胎盘绒毛组？…

扶桑（Ｈｉｂｉｓｃｕｓ ｒｏｓａ－ｓｉｎｅｎｓｉｓＬ．）花石油醚提取物ＨＲ－１对体外培养的人早期盘颈毛组织分泌功能的影响。实验结果表明：ＨＲ－１对人早期胎盘绒毛组织ＨＣＧ和孕酮的分泌、对绒毛滋养层细胞的生长均有较强的抑制作用。     

人α－干扰素基因在小菜蛾细胞中的表达及其影响因素探讨

将含有人α－干扰素（ＩＦＮα）基因的杆状病毒转移载体（ｐＡｃ３７３－ＩＦＮα）与苜蓿银纹夜蛾核型多角体病毒（ＡｃＮＰＶ）ＤＮＡ经钙磷沉淀法共转染小菜蛾细胞系（ＢＣＩＲＬ－Ｐｘ２－ＨＮＵ３,Ｐｘ）,空斑纯化后,利用细胞致病效应（ＣＰＥ）抑制法检测得到了干扰素效价（６８７２．６ＩＵ）,证明得到了可以在Ｐｘ细胞中表达ＩＦＮα的重组病毒（Ａｃ－ＩＦＮα）．并探讨了培养温度和血清浓度对ＩＦＮα表达水平的影响,结果表明：在２８℃时,培养基中补加２％的小牛血清,可获得最高的ＩＦＮα效价（１７４３５．９ＩＵ）．     

干扰素—γ对假孕大鼠黄体功能的影响（Ⅱ）

以假孕大鼠为实验对象,在体注射ＩＦＮ－γ研究ＩＦＮ－γ对血浆孕酮水平的影响,黄体器官离体培养研究ＩＦＮ－γ,ＰＧＦ２α对黄体孕酮水平的作用,结果表明,注射ＩＦＮ－γ后,能提高血浆孕酮水平,由８３．８７ｎｇ／ｍＬ升至１１７．０９ｎｇ／ｍＬ,增长３９．６％（Ｐ〈０．０５）,离体培养条件下加入ＩＦＮ－γ（１２０Ｕ／ｍＬ）也能提高培养液中的孕酮水平（Ｐ〈０．０１）,加入ＰＧＦ２α（１００ｎｇ／ｍＬ）则能显     

砂浆渗浇钢纤维砼轴拉应力－应变全曲线的试验研究

进行了１２根砂浆渗浇钢纤维砼（Ｓｌｕｒｒｙ－ＩｎｆｉｌｔｒａｔｅｄＦｉｂｅｒＣｏｎｃｒｅｔｅＳＩＦＣＯＮ）试件和３个不含钢纤维的对比试件的单调轴向拉伸应力－应变全曲线的试验．证明了国产钢纤维所配制的ＳＩＦＣＯＮ具有很高的抗拉性能．根据实验结果推导了应力－应变各项参数的经验公式和应力－应变全曲线的表达式，可供工程应用参考     

简化Navier－Stokes方程IF理论广义解的存在性和唯一性

根据简化Ｎａｖｉｅｒ－Ｓｔｏｋｅｓ方程ＩＦ理论，提出了一个基本假设，在此假设的基础上研究了ＩＦ理论解的存在性和唯一性问题，考察了当简化Ｎ－Ｓ方程逼近Ｎ－Ｓ方程时，论文结论收敛到Ｎ－Ｓ方程的相应结论，这个结论适用于二维不可压ＩＦ流动。     

鲨鱼软骨血管生成抑制因子的抗体制备与组织特异性表达？…

纯化鉴定了鲨鱼软骨血管生成抑制因子（Ｓｈａｒｋ Ｃａｒｔｉｌａｇｅ－ｄｅｒｉｖｅｄ Ａｎｇｉｏｇｅｎｅｓｉｓ Ｉｎｈｉｂｉｔｏｒｙ Ｆａｃｔｏｒ－Ｉ,ＳＣＡＩＦ－Ｉ）的纯度及分子质量,采用新西兰大白兔背部皮下多点注射法制备了ＳＣＡＩＦ－Ｉ的多克隆抗体,测定了抗体的效价,通过Ｗｅｓｔｅｒｎ ｂｌｏｔ检测,证明了ＳＣＡＩＦ－Ｉ相关蛋白在哺乳动物中的广泛分布及ＳＣＡＩＦ－Ｉ在鲨鱼软骨中的组织特异性表达。     

中华眼镜蛇毒神经生长因子的研究

采用ＣＭ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ和ＳｅｐｈｏｓｉｌＣ８以及ＨＰＬＣ层析方法，自广西产中华眼镜蛇毒中分离纯化神经生长因子（ＮＧＦ），此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明具有促进神经纤维生长的活性。经ＨＰＬＣ及聚丙烯酰胺凝胶电泳鉴定为单一组成，经ＳＤＳＰＡＧＥ及ＨＰＬＣ测定分子量分别为２４．１ＫＤ和２４．９ＫＤ，等电聚焦电泳测得ｐＩ为８．２，氨基酸组分分析表明ＮＧＦ含酸性氨基酸较少，通过１２５｜标记ＮＧＦ测定出ＮＧＦ在生物体内主要分布于肾、肺、及周围神经     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

Jane Eyre——A Feminist In The 19th Century

In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-