Effects of X-irradiation on ribonucleases in blood plasma

Zusammenfassung Unter Bestrahlung wurde die RNAse-Aktivität im Blut untersucht. Es zeigte sich, dass Bestrahlung des Kopfes allein zu einer starken Erhöhung der alkalischen RNAse im Serum führt.     

Effects of the antiprotease Trasylol® on peripheral blood leucocytes

Summary Binding of the antiprotease Trasylol^® to human peripheral blood lymphocytes and polymorphonuclear leucocytes (PMNs) was demonstrated at the ultrastructural level using an indirect immunoperoxidase technique. This also revealed endocytosis of membrane bound Trasylol by PMNs. Trasylol inhibited PHA- and Con A-induced lymphocyte stimulation, and was cytotoxic to unstimulated cells.One of us (D.J.T.) is supported by Bayer Pharmaceuticals Ltd., from whom we also acknowledge a generous supply of Trasylol.     

Effects of the antiprotease Trasylol on peripheral blood leucocytes.

Binding of the antiprotease Trasylol to human peripheral blood lymphocytes and polymorphonuclear leucocytes (PMNs) was demonstrated at the ultrastructural level using an indirect immunoperoxidase technique. This also revealed endocytosis of membrane bound Trasylol by PMNs. Trasylol inhibited PHA- and ConA-induced lymphocyte stimulation, and was cytotoxic to unstimulated cells.     

Effects of environmental temperature-humidity and cage density on body weight and behavior in mice

Zusammenfassung Es wurden Mäuse des C3H/HeJ-Stammes nach Entwöhnung bis zum Alter von 125 Tagen unter verschiedenen Umweltbedingungen wie Temperatur, relative Luftfeuchtigkeit und Käfigdichte aufgezogen. Je höher Temperatur, relative Luftfeuchtigkeit und je grösser die Käfigdichte, um so grösser waren die Körpergewichtsverluste. Umwelttemperatur, Feuchtigkeit und Käfigdichte beeinflussten zusammen das Überleben der Mäuse nach Schwimmen.     

Effects of salicylate on water and electrolyte content of human red blood cells

Zusammenfassung Es wird gezeigt, dass Natriumsalicylatin vitro auf menschliche Erythrozyten einendirekten Einfluss ausübt, indem es den Kaliumgehalt vermindert und den Natriumsowie den Wassergehalt vermehrt. Diese Wirkung, die auchin vivo beobachtet werden kann, dürfte die Folge einer Energieverarmung sein, die durch eine Entkopplung der oxydativen Phosphorylierung zustande kommt.     

Effects of blood sampling,anesthesia and surgery on plasma vasopressin concentration in rats

The influence of blood sampling, anesthesia and surgery on plasma vasopressin concentration was assessed in rats. Mean plasma concentration in conscious, chronically catheterized rats was 1.4±0.1 pg/ml (n=6). This value remained constant over repeated plasma samplings in the same animals. On the other hand, decapitation increased the plasma vasopressin concentration to 6.0±2.4 (in pg/ml) (n=6), inactin anesthesia to 2.9±0.6 (n=6), anesthesia and femoral cannulation to 13.3±5.8 (n=6) and surgery for renal micropuncture to 81.3±35.0 (n=6). It is concluded that the level of circulating plasma vasopressin is highly dependent on the sampling technique and is closely related to the extent of surgery.     

Effects of blood sampling, anesthesia and surgery on plasma vasopressin concentration in rats.

The influence of blood sampling, anesthesia and surgery on plasma vasopressin concentration was assessed in rats. Mean plasma concentration in conscious, chronically catheterized rats was 1.4 +/- 0.1 pg/ml (n = 6). This value remained constant over repeated plasma samplings in the same animals. On the other hand, decapitation increased the plasma vasopressin concentration to 6.0 +/- 2.4 (in pg/ml) (n = 6), inactin anesthesia to 2.9 +/- 0.6 (n = 6), anesthesia and femoral cannulation to 13.3 +/- 5.8 (n = 6) and surgery for renal micropuncture to 81.3 +/- 35.0 (n = 6). It is concluded that the level of circulating plasma vasopressin is highly dependent on the sampling technique and is closely related to the extent of surgery.     

Effects of pyrithiamine and oxythiamine on the thiamine content of tissues and blood pyruvate in mice

Riassunto La Piritiamina, somministrata per bocca in una singola dose di 0,5 mg a topolini di 12–14 g che ricevono 2 µg di tiaminapro die, non produce la sindrome neuromuscolare caratteristica dell'avitaminosi B₁ e non modifica nè la piruvicemia nè il livello di vitamina nel fegato, mentre lo fa abbassare nel muscolo e nel cervello. Se i topolini non ricevono tiamina, la stessa dose di Piritiamina dà perdita di peso, sindrome neuromuscolare in tutti gli animali trattati e cospicuo abbassamento del livello di vitamina B₁ nel cervello. Nelle stesse condizioni sperimentali, l'Ossitiamina, somministrataper os una sola volta in dosi di 0,5 e 2 mg, non dà alcuna sintomatologia neuromuscolare e non modifica nè il peso corporeo, nè il piruvato ematico, nè il contenuto in vitamina B₁ del muscolo e del cervello, solo abbassa il livello vitaminico del fegato.Questi risultati non sono in favore dell'ipotesi di una funzione della tiamina distinta da quella della cocarbossilasi.     

Effect of low density lipoprotein and high density lipoprotein on sodium dodecyl sulphate precipitation of very low density lipoprotein from human serum

Summary In the presence of low density lipoprotein, the sodium sodecyl sulphate(SDS)-very low density lipoprotein(VLDL) complex sedimented, while in the presence of high density lipoprotein the complex floated. This SDS-VLDL aggregate floats at serum triglyceride to cholesterol ratio of 0.7–0.9 and sediments at a ratio of 0.2–0.5.     

Effect of low density lipoprotein and high density lipoprotein on sodium dodecyl sulphate precipitation of very low density lipoprotein from human serum.

In the presence of low density lipoprotein, the sodium sodecyl sulphate(SDS)-very low density lipoprotein(VLDL) complex sedimented, while in the presence of high density lipoprotein the complex floated. This SDS-VLDL aggregate floats at serum triglyceride to cholesterol ratio of 0.7-0.9 and sediments at a ratio of 0.2-0.5.     

Effects of exchange transfusion with perfluorochemical emulsions on hepatic oxygen supply and blood flow in the rat

Exchange-transfusion to hematocrit 20 with isotonic perfluorochemical (PFC) emulsions containing 3% hydroxyethylstarch (HES) in rats breathing 100% oxygen produced significant reductions of hepatic PO2 and blood flow in comparison to rats hemodiluted with isotonic 3% or 6% HES solution. The results indicate that PFC and/or emulsifiers were associated with adverse effects on liver blood supply.     

Effects of exchange transfusion with perfluorochemical emulsions on hepatic oxygen supply and blood flow in the rat

Summary Exchange-transfusion to hematocrit 20 with isotonic perfluorochemical (PFC) emulsions containing 3% hydroxyethylstarch (HES) in rats breathing 100% oxygen produced significant reductions of hepatic PO₂ and blood flow in comparison to rats hemodiluted with isotonic 3% or 6% HES solution. The results indicate that PFC and/or emulsifiers were associated with adverse effects on liver blood supply.This work was supported by a grant from the Kentucky Affiliate of the American Heart Association.     

Effects of oestradiol and progesterone on FSH and LH contents of the pituitaries and blood of ovariectomized ewes

Zusammenfassung Zufuhr von 20 µg Östradiolbenzoat an zwei sich folgenden Tagen verursachte bei ovariektomierten Mutterschafen keine Veränderung im FSH- und LH-Gehalt von Hypophyse und Serum. Nach cinmaliger Verabreichung von 10 mg Progesteron (nach östradiolbehandlung) stieg das FSH im Blut an. Ebenso ergab Zufuhr von 20 mg Progesteron bei mit Östrogen behandelten Mutterschafen Anstieg des LH-Gehaltes im Blut.