血清抗Sm抗体ELISA检测方法的建立

目的：建立血清抗Sm抗体的ELISA检测方法。方法：应用纯化的Sm抗原,采用棋盘滴定等方法确立ELISA方法检测血清抗Sm抗体的最适实验条件。结果：建立了血清抗Sm抗体的ELISA检测方法。SLE患者血清抗Sm抗体的阳性率为29．2％,正常人和其它自身免疫病患者未查到此抗体。结论：本文建立的血清抗Sm抗体检测方法敏感性达到国外进口试剂盒的水平,且操作简便,重复性好,因此临床很有实用价值。     

免疫PCR方法检测乳腺癌患者血清抗p53蛋白抗体

目的为乳腺癌诊断提供血清学新方法.方法免疫PCR方法检测血清抗p53蛋白抗体,酶免疫组化方法检测组织p53蛋白表达.结果乳腺癌患者血清抗p53蛋白抗体阳性率为39.5%,而非癌患者和正常人血清抗p53蛋白抗体均为阴性,乳腺癌患者血清中抗p53蛋白抗体显著高于非癌患者和正常人(P＜0.01).p53蛋白阳性表达的乳腺癌患者抗p53蛋白抗体阳性率为64.2%,明显高于p53蛋白阴性表达组,血清p53抗体测定与p53蛋白表达密切相关(P＜0.01).结论检测血清抗p53蛋白抗体是检测组织p53蛋白理想的替代工具抗p53蛋白抗体可以作为乳腺癌血清学诊断新标志,用于乳腺癌的普查和早期诊断.     

兔抗PEB1多克隆抗体的制备及鉴定

摘要：【目的】利用纯化的空肠弯曲菌表面蛋白PEB1,制备其多克隆抗体。【方法】用PEB1蛋白作为抗原免疫兔,分离血清得到兔抗PEB1多克隆抗体,用ELISA法检测抗体效价,并用Western-Blotting验证。【结果】PEB1蛋白免疫兔后,得到了兔抗PEB1多克隆抗体,间接ELASA法检测表明兔抗PEB 1血清效价达到1：10 000,Western印记检测表明,该抗体能与PEB1蛋白发生特异性结合,其特异性与敏感性均较好。【结论】获得了高效价的PEB1多克隆抗体。     

SLE患者血清ENA抗体的检测及临床意义

系统性红斑狼疮(Systemic Lupus erythematosus, SLE)是一种病因不明的自身免疫病,病人体内由于B淋巴细胞多克隆激活而产生多种自身抗体,这些抗体能与细胞核或胞浆内的许多成分反应,对SLE的诊断有重要意义,如抗ds—DNA抗体,抗组蛋白抗体,抗Sm抗体等。由Halman等(1959)首先报导某些SLE患者血清中有一种自身抗体,能和细胞核的盐水可溶性成分反应,命名为可提取核抗原(extractable nuclear antigen, ENA)抗体。后经学者研究,发现ENA含多种抗原成分,如Sm、RNP、SSA、SSB、ScL—70、Jo—1、Ku、HSP—90等,其相应抗体对风湿病的诊断和鉴别诊断有重要价值,如抗Sm抗体对SLE特异性较高,高滴度RNP抗体对混合性结缔组织病(MCTD)特异性较高,SSA及SSB抗体是干燥综合征(SS)特异性抗体。研究较多的是Sm和RNP抗体。 1 ENA抗原的分子特性 ENA属非组蛋白核蛋白,因其大多数是酸性蛋白,所以又称酸性核蛋白。ENA常从兔或小牛胸腺、猪胸腺、Hela细胞、Wi1—2细     

痤疮丙酸杆菌对小鼠抗胸膜肺炎放线杆菌血清7型9型的免疫保护

为明确痤疮丙酸杆菌(PA)对具有抗胸膜肺炎放线杆菌(APP)血清7型和血清9型的异源免疫保护作用,对小鼠免疫PA分离株S14后,分别用APP血清7型(CCVC-265)和血清9型(CCVC-267)攻毒。结果表明,免疫保护率达60%和70%;而用猪抗PA血清免疫小鼠后以APP血清7型和血清9型攻毒,免疫保护率达100%。血清特异性抗体效价检测显示,PA免疫小鼠后,可诱导小鼠产生特异性的抗APP血清7型和9型的抗体,ELISA方法检测特异性抗体平均效价为1 800和2 800。PA对APP血清7型和9型的感染具有良好的保护作用。     

酶联免疫吸附分析(ELISA)检测抗ss-DNA自身抗体

本实验用小牛胸腺单股DNA(ss-DNA),通过酶联免疫吸附分析(ELISA)测定了可疑全身性红斑狼疮(SLE)及相关患者血清标本21个,其中7个病人血清与ss-DNA反应阳性,存在抗ss-DNA自身抗体。这7个病人血清在SLISA检测O.D值比健康人对照血清的O.D值高出1.5倍以上。方法简便、准确,重复性好,对SLE病人暴发前检测具有参考价值。     

唾液IgG抗体测定在幽门螺杆菌感染诊断中的价值

目的 :探讨唾液抗幽门螺杆菌(Helicobacterpylori,Hp)IgG测定对Hp感染的诊断价值。方法 :用ELISA法测定67例Hp阳性及20例Hp阴性患者的唾液内抗HpIgG ,并与血清抗体测定结果比较。结果 :唾液抗HpIgG对Hp感染的敏感性为91 1% ,特异性为85 0% ,准确性为89 7 % ,与血清测定结果接近(分别为92 6 % ,90 0%和91 9 %)。结论 :唾液抗Hp抗体测定对Hp感染的诊断有较好的应用价值。     

丙型肝炎病毒抗体免疫酶快速检测试剂的评价

对丙型肝炎病毒抗体免疫酶快速检测试剂(IEA)检测丙型肝炎病毒抗体(HCV-Ab)的性能、结果等技术参数进行评价,为实验室的选用提供参考.采用IEA法、ELISA法和胶体金法对中国生物制品检定所“第三代ELISAHCV-Ab诊断试剂国家参考品”和3297份血清或血浆标本同时测定,对IEA的敏感性、特异性、重复性和检测时间等结果进行评价.结果表明:IEA可通过中国生物制品检定所“第三代ELISAHCV-Ab诊断试剂国家参考品”检验;与ELISA法比较,IEA的检出率为99.06%,灵敏度与ELISA相当,胶体金的检出率为86.01%,灵敏度比ELISA低;IEA检测所需时间15～25min.IEA既有ELISA的敏感性、特异性,又有胶体金简便、快速的特点.适用于献血员及高危人群中HCV抗体的筛查和临床诊断HCV感染,以及门诊、急诊和基层医疗单位的现场检测.     

酶联免疫双抗夹心测定Ⅳ型胶原蛋白

目的：建立血清Ⅳ型胶原蛋白（COLW）浓度测定的酶联免疫试验（ELISA）方法,辅助诊断肝硬化。方法：制备过氧化物酶（HRP）-免抗人COLⅣ多抗结合物,作为标记抗体;用棋盘滴定试验确定包被抗体和标记抗体浓度,进行检出限、精密度、回收率、特异性、灵敏度测定和标准曲线的制备;初步用于79例正常人和34例肝纤维化患者。结果：COLⅣ浓度在40~I000μg／L之间时,该法工作曲线最佳,最低检出限为40μg／L,批内异变系数（CV）6．098％,批间CV4．30％,高、低浓度回收率分别为95％和98％。该法测定正常人COLⅣ浓度平均为67.6±24.6μg／L,而肝纤维化患者平均水平为215.96％±96μg／L,可见辅助诊断肝纤维化具有很高特异性和灵敏度。结论：ELISA方法检测定血清COLⅣ简便、特异、准确、价格低廉,可用于肝纤维化辅助诊断并在国内推广应用。     

左氧氟沙星人工抗原的制备及其抗体鉴定

用N-羟基琥珀酰亚胺法把左氧氟沙星与载体蛋白牛血清白蛋白偶联制备免疫抗原,用氯甲酸异丁酯法将左氧氟沙星与鸡卵清蛋白偶联,制备包被检测抗原,采用紫外扫描法对合成的抗原进行鉴定.用免疫抗原免疫大白兔获得抗左氧氟沙星多克隆抗体血清,经间接ELISA试验检测特异性抗体效价,测得效价达1:212以上.通过紫外扫描及间接ELISA试验,结果表明,制备的抗原和抗体获得成功.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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