Structure of the ovipositor,associated sensilla and spermathecal system of entomoparasitic pipunculid flies (Diptera Pipunculidae)

The external parts of the female reproductive system in entomoparasitic flies of the family Pipunculidae form a compact, heavily sclerotized ovipositor for inserting the egg into the host. Two types of pipunculid ovipositor have been recognized: The Nephrocerus type is bilaterally asymmetrical and consists of modified abdominal segments 8 and 9. There is no spiracle. The genital opening is situate ventrally, approximately in the middle of the ovipositor. The Pipunculus type ovipositor is bilaterally symmetrical and originates from abdominal segments 7, 8 and 9. A pair of spiracles are located venterolaterally near the basal margin; the genital opening is situated close to the apex. Two types of campaniform and two types of haired sensilla have been found (tergum 9 only). Campaniform sensilla have been observed in apical third to apical half of the ovipositor of all studied species. Ten long haired sensilla arranged in two lateral rows on apex have been recognized only in species of Nephrocerus. Short haired sensilla have been found in all Chalarinae and Pipunculinae, situated in basal half of the ovipositor piercer. The spermathecal system consists of three spermathecae; well sclerotized (Nephrocerinae, Chalarinae) or soft and membraneous (Pipunculinae); and three spermiducts which can be undifferentiated and tubular (Nephrocerus) or differentiated to muscular, glandular and ductal parts (Chalarinae, Pipunculinae). In the Nephrocerus type ovipositor, spermathecae are situated in the distal half of abdominal segment 6; all other Pipunculidae have spermathecae located in the basal part of the ovipositor.     

Ovipositor mechanics of the braconine wasp genus Zaglyptogastra and the ichneumonid genus Pristomerus

The multiply-arched, apical quarter to half of the ovipositors of Zaglyptogastra species are capable of flexing ventrally through up to 180°. Flexion is achieved by ventral protrusion of the lower ovipositor valves relative to the upper one, an action which juxtaposes swollen lower valve sections with weak concave narrowings of the upper valve. The structural modifications associated with this flexion mechanism are described and similar ovipositor features are illustrated for the cremastine ichneumonid genus Pristomerus.     

Description of Torymus lasallei,sp. nov. (Hymenoptera: Torymidae), a species with an unusual ovipositor

ABSTRACT

Torymus lasallei, sp. nov., a species with an unusual ovipositor, reared from galls of Amphibolips spp. (Hymenoptera: Cynipidae) on Quercus spp. (Lobatae section) from Mexico, is described. The placement of this new species within the genus Torymus, and the morphology and function of its ovipositor, are discussed.

http://www.zoobank.org/urn:lsid:zoobank.org:pub:0B9F9B11-DD95-465D-A98C-53742A8099CC     

Phylogenetic implications of ovipositor structure in Eucharitidae and Perilampidae (Hymenoptera: Chalcidoidea)

The external and cross-sectional morphology of the ovipositor was examined for 21 genera and 28 species of Philomidinae, Perilampidae and Eucharitidae. Eleven characters were scored and either mapped onto existing topologies reflecting the relationships of genera, or included in a larger data set focused on the generic relationships of Eucharitidae and analyzed using parsimony. The presence of a setiform flap in the ventral wall of the dorsal valve supported the monophyly of Perilampinae and possibly Chrysomalla (Chrysolampinae) + Perilampinae. Monophyly of Eucharitidae was supported by a thinning of the dorsal wall of the dorsal valve. One of two islands of most parsimonious trees that have recently been proposed was selected on the basis of fewer step changes as a result of both character mapping and parsimony analysis. Within Eucharitinae, this would support a clade that includes Kapala, Stilbula and Pseudochalcura, with Schizaspidia and Chalcura as its sister group. By mapping characters, an additional hypothesis of monophyly of Anorasema and Gollumiella was preferred, which on the basis of the pruned topologies was a single step shorter than the preferred islands of trees.     

Revision of the Australian endemic genus Hyptiogaster Kieffer (Hymenoptera: Gasteruptiidae), with descriptions of seven new species

The Australian hyptiogastrine wasp genus Hyptiogaster Kieffer is revised. Among other characters, species of Hyptiogaster can be readily distinguished from other Hyptiogastrinae by having an exserted ovipositor and the anterior face of the mesothorax truncate in lateral view. Ten species are recognised including seven which are newly described: H. arenicola Turner, H. humeralis (Schletterer), H. rufus (Westwood), H. cobarensis sp. nov., H. hulli sp. nov., H. kalbarrii sp. nov., H. kiefferi sp. nov., H. naumanni sp. nov., H. pinjarregaensis sp. nov., H. weowaniensis sp. nov. Two synonymies are proposed; H. flavosignata (Kieffer) with H. humeralis and H. crassiceps (Schletterer) with H. rufus. An illustrated key to species is presented and relationships among genera of Hyptiogastrinae and species of Hyptiogaster are discussed.     

New diagnostic ovipositor characters for doryctine wasps (Hymenoptera,Braconidae)

Characters previously used to distinguish the braconid wasp subfamily Doryctinae show considerable variation. Here we illustrate three new female external adult characters that will facilitate recognition of doryctines and allow precise definition of the subfamily. The new characters are a distinctively shaped apex to the dorsal ovipositor valve, a modified serration structure of the lower ovipositor valve, and a particularly heavily sclerotized ovipositor apex. As a result of finding additional synapomorphies linking the Ypsistocerinae with the Doryctinae, the former group is reduced in rank to a tribe within the latter.     

The occurrence and phylogenetic implications of the ovipositor clip within the Figitidae (Insecta: Hymenoptera: Cynipoidea)

The presence of the ovipositor clip is surveyed throughout Figitidae. This morphological structure is postulated to restrain the parasitoid host during oviposition. All Figitinae and Eucoilinae that attack semi‐concealed dipterous hosts were found to possess the clip. Figitids that attack fully concealed hosts all lacked the ovipositor clip. It is hypothesized here that two subfamilies, the Anacharitinae and Aspicerinae, attack fully exposed hosts yet lack the clip in order to quickly oviposit and prevent fighting with the host. Mapping of the presence/absence of the ovipositor clip on to two competing phylogenies suggests this structure evolved in parallel in Figitinae and Eucoilinae. The evolution of the ovipositor clip is probably responsible for the successful colonization of semi‐concealed dipterous larvae by Eucoilinae.     

Terellia fuscicornis (Diptera: Tephritidae): biological and morphological adaptation on artichoke and milk thistle

The biology, morphology, and genetics of Terellia fuscicornis on cultivated artichoke and its new recorded host, Silybum marianum, are examined. Morphometrically, the two host‐associated populations were distinct. Except for egg length, all immature stages of the artichoke‐associated population were larger. Principal component analysis, based on head and wing measurements, showed that the adults from both populations clustered into two groups, with the adults reared from artichoke being larger. The ovipositor was significantly longer and blunter in the artichoke‐associated females, reflecting the larger flower heads exploited. Male terminalia measurements showed significant differences between the two populations. Several differences in courtship behaviour were detected between the two populations. Although the two populations show allochronic isolation, sequencing of two mitochondrial genes, ND1 and COI, and one nuclear gene, 18S, revealed no genetic differences between the two populations.     

First record of a non-pollinating fig wasp (Hymenoptera: Sycophaginae) from Dominican amber,with estimation of the size of its host figs

Fig trees and their pollinating fig wasps arose about 75 million years ago in the Cretaceous period. Several other groups of chalcid wasps also utilize figs for larval development, including sycophagines, the putative sister group to pollinating fig wasps. Whereas stone and amber fossil pollinators are known, no fossils representing non-pollinating fig wasp groups have been confirmed previously. Here, we describe the first Sycophaginae from the c.15–20 Ma Dominican amber, Idarnes thanatos sp. nov. Farache, Rasplus, Pereira and Compton, and discuss its relationships within the Idarnes carme species group. Additionally, we use linear regression to compare body size, ovipositor sheaths length, and host fig size data from extant Idarnes species to estimate the size of its host figs. Idarnes thanatos was most likely associated with small to medium sized figs (diameter ≤1.0 cm), that were likely to have been dispersed by birds and primates. The discovery of this close relative of extant non-pollinating fig wasps suggests that early Miocene and modern fig wasp communities may share similar ecological and functional features.     

A morphological study of Phyllotreta tetrastigma (Coleoptera: Chrysomelidae: Alticinae) including two colour forms

P. tetrastigma is a chrysomelid beetle with two colour forms, and has populations feeding on two different host plants. Morphological variation was studied within both males and females of six populations of P. tetrastigma. Morphological difference was detected between the sexes and between populations from different localities but not between the colour forms. It is not clear whether host plant influences morphology.     

Patterns of host-plant utilization by leafhoppers in the genus Eupteryx (Hemiptera: Cicadellidae) in Britain

Known host-plant associations are listed for the 16 species of Eupteryx occurring in Britain. A total of 77 species in 17 plant families are exploited. The most frequently represented plant family is the Labiatae. Degrees of specificity range from strict monophagy (four spp.) to broad polyphagy (five spp.). In general, host-plant overlap between species is low. The detailed relationships between three Eupteryx species and three members of the Urticaceae were explored using field populations and laboratory feeding experiments, survival tests and examination of patterns of oviposition. Eupteryx urticae, E. cyclops and E. aurata all utilized Urtica dioica as their primary host plant. However, the only regularly recorded association with other members of the Urticaceae was the host range expansion of E. urticae onto Parietaria judaica in the second generation. These findings were supported by the laboratory experiments. The spatial distributions of E. urticae oviposition sites and levels of attack by mymarid parasitoids were similar in populations on the two plant species. The implications of these results are discussed.     

Nesting habits of the Japanese foliage spider,Cheiracanthium japonicum (Araneae: Miturgidae): host plant preference based on the physical traits of plant leaves

A Japanese foliage spider, Cheiracanthium japonicum, builds nests with plant leaves. The nests are classified into seven types in relation to the seasons and purposes of nesting. We investigated whether the spider selects a plant species according to the nest type and how the physical traits of leaves influence the spider's plant selection. The difference between the composition of the host plant species used for nesting and that of the seasonal vegetation cover was confirmed. This suggests that the spider chooses the host plant species regardless of its abundance in their habitat. Early juveniles use small living or large dead leaves of various plant species to build moulting nests. Females prefer long and large leaves found in Miscanthus sinensis and Phragmites japonica over other plant leaves to build breeding nests.     

Description of the biology and immature stages of Pagyris ulla (Hewitson) (Lepidoptera: Nymphalidae: Ithomiini) in the Colombian Andes

The biology and immature stages of Pagyris ulla (Hewitson, [1857]) (Lepidoptera: Nymphalidae: Ithomiini) are described for the first time. The species’ host plant is established to be Brugmansia candida Pers. (Solanaceae). The life cycle from eggs to adult under laboratory conditions and ambient temperature took approximately 47 days, and the larvae passed through five instars. The larvae are gregarious, feed at night, and rest during the day in nests made by joining leaves near the apex of the plant stem.     

Plant consumption in coastal populations of the lizard Tropidurus torquatus (Reptilia: Squamata: Tropiduridae): how do herbivory rates vary along their geographic range?

Tropidurus torquatus lizards commonly consume vegetal matter as part of their diet. However, it is not known to what extent the rate of consumption of plant material varies among populations. We investigated the consumption of plant material in 10 populations of T. torquatus in eastern Brazil. In all populations, lizards consumed plant matter (especially fruits and flowers). The proportion of plant volume in the diet varied from 2.1% to 58.0% of the total volume consumed. Differences in volumetric proportion of plant material consumed did not result from lizard body size or population latitude. The volumetric proportion of plant material did not differ between sexes at each locality; however, there was a slight trend for larger lizards to consume more plant parts. The data obtained indicate that the observed interpopulational variation in plant consumption by T. torquatus results from local environmental factors, mainly the availability of plant matter.     

XXVIII.—Les Trechus [Coleoptera,Carabidæ] de l'Everest Expedition, 1924, et Remarques sur quelques espèces de l'Himalaya

Summary

Chorthippus m. montanus and C. p. parallelus are often difficult and sometimes impossible to separate using existing keys. Eleven potentially useful characters are re-evaluated in the male and six in the female. In males the best characters are found to be the number of stridulatory pegs on the hind femur, the length of the stridulatory file, cercus and anterior medial area, and the distance of the stigma from the fore wing tip; these characters are closely correlated with each other. In females the shape and length of the ventral valves of the ovipositor are found to be the most useful characters. The acoustic behaviour, cytology, serology, ecology and distribution of the two species are discussed.

A lectotype is designated for C. caffer Ramme, which is newly synonymised with C. p. parallelus. C. erythropus Faber is reduced in status to a subspecies of C. parallelus. The status of the following is also discussed: C. montanus tatrae Harz, C. parallelus aemulus Mishchenko, C. p. geminus Mishchenko, C. p. tenuis (Brullé), C. turanicus Tarbinskii and C. curtipennis (Harris).

A key is given to the Western European species and subspecies of this group.     

Immature stages of the apterous fly Badisis ambulans McAlpine (Diptera: Micropezidae)

Morphology of the mature larvae and pupae of the apterous fly, Badisis ambulans McAlpine (Diptera: Micropezidae) are described and illustrated. In addition, the curious commensal habits of the larvae are described. Each spiracular plate of the mature larva is situated at the base of a small horn; similar horns have been described in other micropezid larvae. The posterior spiracles appear to be non-functional with depressions forming the vestiges of the spiracular openings. The larvae were found inside pitchers of the Albany pitcher plant, Cephalotus follicularis (Cephalotaceae) and have a commensal relationship with the plant. They feed on the decaying pitcher plant prey and leave the pitcher to pupate. It is likely that the larva obtains oxygen from the pitcher fluid. This is the first immature micropezid described from Australia.     

Feeding ecology of the common sun skink,Eutropis multifasciata (Reptilia: Squamata: Scincidae), in the plains of central Vietnam

We studied the feeding ecology of Eutropis multifasciata in the tropical plains of central Vietnam to understand better the foraging mode, spatiotemporal and sexual variation in dietary composition, and rarefaction curves of prey-taxon richness for males and females. Stomach contents (n = 161) were collected from October 2013 to May 2014 using a nonlethal stomach-flushing technique. A total of 680 food items (624 animal items and 56 plant items) was found in 161 stomachs of skinks, representing 19 unique animal categories. We found that the diet of E. multifasciata is composed mainly of small, sedentary and clumped prey and that this skink specialises on spiders, insect larvae, snails, grasshoppers and crickets (with a combined importance index of 60%). Dietary composition, prey size and total prey volume in E. multifasciata changed between dry and rainy seasons and among regions. The total volume of food items consumed by males was larger than that of females, and the diversity and evenness index of prey categories were larger in males than in females. However, using rarefaction curves revealed that females have the higher prey-taxon richness after points between 130 and 140 prey items for frequency, and between 160 and 170 prey items for number of items, and the differences were not statistically significant. The foraging behaviour of E. multifasciata best fits a ‘widely foraging’ model.     

Observations on zygopine weevil behaviour (Coleoptera: Curculionidae: Zygopinae)

Observations on the behaviour and chosen environment are reported of the zygopine weevils Mecopus audinetii, M. bispinosus, M. fausti, M. pulvereus, Phylaitis v-alba and Osphilia sp. Mecopus audinetii and M. bispinosus were not distinguished in the field, but can be separated by details of the male and female genitalia and by the presence in the male of M. bispinosus of long black hairs on the pronotum. All the species studied were found on the bark of fallen or dying trees, the Mecopus species and Phylaitis v-alba being associated with whitish-silvery bark and the Osphilia species with greenish-brown substrates. The small Phylaitis species was found on branches of smaller diameter than those frequented by the larger Mecopus species. All the species are fairly cryptic in coloration, but when moving the larger species are more visible than the smaller, and the ones on pale bark are more visible than those on greenish-brown bark. Potential predators may be confused by jerky movement patterns observed in all species and the distant shadows cast by the long-legged larger Mecopus. The weevils are not believed to be involved in any mimicry, although this has been suggested for other members of the same subfamily. A single contest between males of Mecopus audinetii/bispinosus was observed, in the absence of a female. Each male attempted to dislodge its opponent from the substrate using its rostrum; the thoracic spines were not seen to be used. Contests between males of Osphilia sp. and Phylaitis v-alba were observed in the presence of females of the appropriate species. The males of Osphilia species attempted to dislodge one another from the back of the female in contention, and also engaged in a spiralling flight together. The males of Phylaitis v-alba did not come into physical contact, but responded to the threat of an approach. During copulation in Mecopus audinetii/bispinosus and Osphilia sp. the male repeatedly brushed the head and thorax of the female with his front legs. In the first species the male stood on the substrate and ‘caged’ the female with his legs and rostrum but in the second the male, ‘rode’ the female. Both species oviposit into the bark of their trees, the females using the rostrum to drill a hole for the ovipositor. The length of the rostrum probably determines the thickness of the bark through which eggs are laid, and hence the area of the region of the tree inhabited by the beetles.     

Meteorus acerbiavorus sp. nov. (Hymenoptera,Braconidae), a gregarious parasitoid of Acerbia alpina (Quensel) (Lepidoptera,Arctiidae) in North Finland

The new gregarious parasitoid Meteorus acerbiavorus sp. nov. (Hymenoptera, Braconidae) was reared from the cocoons of Acerbia alpina (Quensel) (Lepidoptera, Arctiidae) in north-western Finnish Lapland. This species belongs to Meteorus rubens (Nees) species group and differs from the most related M. rubens in the following features: the eyes densely setose; the median lobe of the mesoscutum, scutellum, mesopleuron, and the hind coxa entirely or at least partly rugulose-granulate or rugose-areolate and sometimes with granulation; the ovipositor subapically with distinct dorsal node; the ventral borders of the first metasomal tergum weakly separated by narrow space in its basal half; the colour of the body and legs mostly or entirely dark; the fore wing more or less darkened. Phylogenetic relationships among several Meteorus species close to M. rubens including new M. acerbiavorus were investigated based on DNA sequence fragments of the mitochondrial COI and the nuclear 28S rDNA genes. The discussions on the species groups of Meteorus, on distribution of Acerbia alpina in the Holarctic and on its known parasitoids are presented.