Isoelectric fractionation of desialyzed interferon

Riassunto Proteine urinarie di coniglio contenenti interferone sono state desializzate mediante neuraminidasi e sono state separate simultaneamente ai controlli mediante elettroforesi su poliacrilammide con gradiente di pH. La rimozione dell'acido sialico produce una notevole modificazione del profilo elettroforetico con diminuzione dell'attività interferonica avente punti isoelettrici inferiori a pH 6.3. Il risultato indica che almeno in parte l'interferone contiene acido sialico.

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This work was supported by a grant from the Consiglio Nazionale delle Ricerche, Roma, Gruppo Nazionale di Medicina Sperimentale.     

A new approach to preparation and purification of colostrokinin from bovine colostrum

Summary A simple and rapid method is reported for preparation and partial purification of colostrokinin from bovine colostral whey. The active substance obtained at the described stage of purification appears to be a basic polypeptide of low molecular weight. This peptide proved to be undestroyed by pepsin, only partially by trypsin and completely by papain.Acknowledgment. This research was partially supported by the Italian Ministry of Health.     

Absence of uricolytic activity in human parotid glands

Zusammenfassung In homogenisierter menschlicher Speicheldrüse liess sich entgegen den Angaben vonStern undIglesias ⁴ keine Urikase nachweisen.

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This work was performed during the tenure of Grants A 139 and A 139-C from the National Institute of Arthritis and Metabolic Disease, Department of Health, Education and Welfare, Public Health Service.     

The fractionation of arginine-rich histones from calf thymus

Zusammenfassung Die argininreiche Fraktion des Kalbsthymushistons (Fraktion 2a) wurde in zwei Komponenten zerlegt. Zusammensetzung und elektrophoretische Trennung der Komponenten (F2a_I und F2a_II), ebenso die Eigenschaften eines neuen, lipidgebundenen, serinreichen Histons wurden beschrieben.     

Degeneration secretion from the parotid gland of the dog

Zusammenfassung In der Parotisdrüse von Hunden erscheint eine kurz anhaltende sogenannte Degenerations-sekretion 2–4 Tage nach postganglionär-parasympatischer Denervierung.     

Parasympathetically evoked parotid salivary secretion of chronically amitriptyline-treated rats

Summary Treatment of rats with amitriptyline for 4 weeks significantly decreased flow rate of saliva elicited from parotid glands in response to electrical stimulation of the parasympathetic innervation but did not alter calcium concentration of such saliva. The mechanism of the dissociation between flow rate and calcium concentration of parasympathetically evoked saliva induced by amitriptyline treatment remains to be explored, and may not involve an amitriptyline induced reduction in acetylcholine release.Acknowledgments. This work was supported by NIDR grant No. DE 02110. The authors wish to thank Dr C. A. Stone of Merck, Sharp and Dohme, Inc., West Point Pennsylvania 19466 for kindly supplying the amitriptyline used in these experiments.     

On the adrenergic innervation of the rat parotid gland

Summary In parotid glands of rats, some adrenergic nerves persisted after avulsion of the superior cervical ganglion, while in submaxillary glands, the adrenergic nerves disappeared completely.This work was supported by grants from the Faculty of Medicine in Lund to J. E.     

Permeability of cellophane membranes to parotid during dialysis.

Pooled paratoid saliva was dialyzed in cellophane membranes against water for periods of up to 1 week and loss of proteins was monitored by acrylamide gel-electrophoresis. A gradual loss of cationic proteins was observed whereas anionic proteins were not appreciably affected. Loss of the cationic proteins could be greatly reduced by performing dialyses against dilute electrolyte solutions rather than water. These effects were attributed primarily to electrostatic changes associated with the dialysis membranes.     

Permeability of cellophane membranes to parotid proteins during dialysis

Summary Pooled parotid saliva was dialyzed in cellophane membranes against water for periods of up to 1 week and loss of proteins was monitored by acrylamide gel-electrophoresis. A gradual loss of cationic proteins was observed whereas anionic proteins were not appreciably affected. Loss of the cationic proteins could be greatly reduced by performing dialyses against dilute electrolyte solutions rather than water. These effects were attributed primarily to electrostatic charges associated with the dialysis membranes.The assistance of Mr E. Pederson and Dr G. Clark is gratefully acknowledged. Supported by Naval Medical Research and Development Command Project MR005.19 6048, Bethesda, Maryland. The opinions expressed herein are those of the authors and are not be construed as reflecting the views of the Navy Department or the Naval Service at large. The use of commercially available products does not imply endorsement of these products or preference to other similar products on the market.Deceased.     

Blood flow through the parotid gland of the cat

Zusammenfassung Eine Vasodilatation erscheint in der Parotisdrüse der Katze gleichzeitig mit sekretorischer Aktivität (Degenerationssekretion oder Sekretion, hervorgerufen durch Stimulierung der Jacobsonschen Anastomose). Atropin blockiert die Vasodilatation im ersten, nicht aber im zweiten Falle. Das Resultat weist auf spezifische Vasodilatatoren hin.