Long-term production and culture of clones of human T-lymphocytes

Culture of blood T lymphocytes collected from normal individuals and cancer patients were carried out in presence of T cell growth factor (TCGF); these cultures presented cytotoxic activity directed against different targets (lectin activated cells, autologous cancer cells, antibody coated cells and K 562). In order to study separately the different effector subpopulations, isolation of single cultured cells were performed with the help of a micropipette under microscope and monoclonal cultures were carried out in presence of TCGF. In the preliminary cytotoxic assays performed in the clones: (1) a marked activity directed against lectin targets was observed in many clones and (2) an important N K activity was exhibited by the clone 45 B9 (65% of the tested cells lysed human lymphoma K 562 cells).     

Inhibition of collagen synthesis by interleukin-1 in three-dimensional collagen lattice cultures of fibroblasts

Interleukin-1 (Il-1) was added to collagen lattice cultures of human skin fibroblasts. No cell division was induced, the ability of fibroblasts to contract the lattices was decreased and a dose-related inhibition of collagen synthesis without effect on non-collagen proteins was found. Indomethacin had no influence on these effects.     

Inhibition of collagen synthesis by interleukin-1 in three-dimensional collagen lattice cultures of fibroblasts

Summary Interleukin-1 (II-1) was added to collagen lattice cultures of human skin fibroblasts. No cell division was induced, the ability of fibroblasts to contract the lattices was decreased and a dose-related inhibition of collagen synthesis without effect on non-collagen proteins was found. Indomethacin had no influence on these effects.     

Action of cortisone on human fibroblasts in vitro

Résumé Deux sources de fibroblastes originaires de poumon d'embryon humain ont été cultivées en milieu de culture contenant 2,5 g/ml de cortisone. L'hormone a prolongé la vie des souches et stimulé la division cellulaire.     

The growth of human fibroblasts and A431 epidermoid carcinoma cells on gamma-irradiated human amnion collagen substrata

Human fibroblasts and A431 human epidermoid carcinoma cells were cultured on gamma-irradiated human amnion collagen as well as on plastic dishes and non-irradiated collagen coated dishes. The morphology, attachment, growth and short-term cytotoxicity of these culture conditions have been determined. Both irradiated and non-irradiated amnion collagen enhanced the attachment and proliferation of fibroblasts as compared to the plastic dishes. No differences in these properties were observed for A431 cells cultured on irradiated collagen when compared with culture on non-irradiated collagen substrates. Cytotoxicity assays showed that irradiated and non-irradiated collagens were not cytotoxic for either fibroblasts or A431 cells. The results demonstrated that amnion collagen irradiated at doses of 0.25-2.0 Mrads is optimal for cell growth.     

The growth of human fibroblasts and A431 epidermoid carcinoma cells on gamma-irradiated human amnion collagen substrata

Summary Human fibroblasts and A431 human epidermoid carcinoma cells were cultured on gamma-irradiated human amnion collagen as well as on plastic dishes and non-irradiated collagen coated dishes. The morphology, attachment, growth and short-term cytotoxicity of these culture conditions have been determined. Both irradiated and non-irradiated amnion collagen enhanced the attachment and proliferation of fibroblasts as compared to the plastic dishes. No differences in these properties were observed for A431 cells cultured on irradiated collagen when compared with culture on non-irradiated collagen substrates. Cytotoxicity assays showed that irradiated and non-irradiated collagens were not cytotoxic for either fibroblasts or A431 cells. The results demonstrated that amnion collagen irradiated at doses of 0.25–2.0 Mrads is optimal for cell growth.     

Determination of polyamine oxidase activities in human tissues

A very simple fluorometric assay for polyamine oxidase (PAO) in tissues, with N1-monoacetylspermine as substrate, is described. The PAO was present in all human organs tested; it was highest in the liver, followed by the testis, kidney, spleen and small intestine.     

Determination of polyamine oxidase activities in human tissues

Summary A very simple fluorometric assay for polyamine oxidase (PAO) in tissues, with N¹-monoacetylspermine as substrate, is described. The PAO was present in all human organs tested; it was highest in the liver, followed by the testis, kidney, spleen and small intestine.     

Isolation of a growth-stimulating agent from human skin fibroblast cultures

Summary Cell-free supernatants were harvested from cultures of human skin fibroblasts, were applied on to DEAE-cellulose columns, and the first fraction eluted with phosphate-buffered saline contained the growth-stimulating agent. The eluted fraction was then passed through a series of amicon membranes. After passing through PM-10, the filtrate stimulated growth of bovine vascular endothelial, canine myocardial, and human mammary carcinoma cells.     

Toxicity of rabbit anti-dog lymphocyte serum to human cell cultures

Zusammenfassung Die Kombination von Kaninchen Anti-Hund Lymphozytenserum und Kaninchen Komplement war für menschliche Blutzellen in in-vitro-Kultur sowie für. Blutlymphozyten von Patienten mit chronischer lymphatischer Leukämie toxisch.     

Cytotoxicity of human peripheral blood T-lymphocyte clones activated by hepatitis B virus surface antigen

Summary The present studies examined the cytotoxic activities of peripheral blood lymphocytes (PBL) from volunteers with (sero-positive) and without (sero-negative) circulating antibodies to hepatitis B virus surface antigen before and 30 days after vaccination with hepatitis B virus surface antigen (HBsAg). Long-term culture of monospecific hepatitis B surface (HBsAg)-responsive T-lymphocytes were isolated and grown in large numbers. The mechanism of T-cell mediated cytolysis, and the identification of the carbohydrate determinants on the surface of these effector cells responsible for the killing effect, are being examined.     

Cytotoxicity of human peripheral blood T-lymphocyte clones activated by hepatitis B virus surface antigen

The present studies examined the cytotoxic activities of peripheral blood lymphocytes (PBL) from volunteers with (sero-positive) and without (sero-negative) circulating antibodies to hepatitis B virus surface antigen before and 30 days after vaccination with hepatitis B virus surface antigen (HBsAg). Long-term culture of monospecific hepatitis B surface (HBsAg)-responsive T-lymphocytes were isolated and grown in large numbers. The mechanism of T-cell mediated cytolysis, and the identification of the carbohydrate determinants on the surface of these effector cells responsible for the killing effect, are being examined.