Cytotaxonomy of the myobatrachid frogs of the genusLimnodynastes

Summary 4 of the 5 species ofLimnodynastes of theperoni group show 2n=24. Onlysalmini has 2n=22, thus resembling the species ofPlatyplectron (thedorsalis andornatus complexes) which all show 22 chromosomes. This 2nd group also includes species (ornatus) with very low DNA amounts. From a karyological point of view, the species of thePlatyplectron (plus eventuallysalmini) group appear more differentiated than the species of theperoni group, from which they have probably arisen.Research partly supported by a contribution from C.N.R., Rome.     

Forecasting for the generation of trading signals in financial markets

In this paper we show that optimal trading results can be achieved if we can forecast a key summary statistic of future prices. Consider the following optimization problem. Let the return r_i (over time i=1, 2, ..., n) for the ith day be given and the investor has to make investment decision d_i on the ith day with d_i=1 representing a ‘long' position and d_i=0 a ‘neutral' position. The investment return is given by r=Σⁿ_i=1r_id_i−cΣⁿ⁺¹_i=1∣d_i−d_i−1∣, where c is the transaction cost. The mathematical programming problem of choosing d₁, ..., d_n to maximize r under a given transaction cost c is shown to have an analytic solution, which is a function of a key summary statistic called the largest change before reversal. The largest change before reversal is recommended to be used as an output in a neural network for the generation of trading signals. When neural network forecasting is applied to a dataset of Hang Seng Index Futures Contract traded in Hong Kong, it is shown that forecasting the largest change before reversal outperforms the k‐step‐ahead forecast in achieving higher trading profits. Copyright © 2000 John Wiley & Sons, Ltd.     

Explaining Leibniz equivalence as difference of non-inertial appearances: Dis-solution of the Hole Argument and physical individuation of point-events

“The last remnant of physical objectivity of space–time” is disclosed in the case of a continuous family of spatially non-compact models of general relativity (GR). The physical individuation of point-events is furnished by the autonomous degrees of freedom of the gravitational field (viz., the Dirac observables) which represent—as it were—the ontic part of the metric field. The physical role of the epistemic part (viz. the gauge variables) is likewise clarified as embodying the unavoidable non-inertial aspects of GR. At the end the philosophical import of the Hole Argument is substantially weakened and in fact the Argument itself dissolved, while a specific four-dimensional holistic and structuralist view of space–time (called point-structuralism) emerges, including elements common to the tradition of both substantivalism and relationism. The observables of our models undergo real temporal change: this gives new evidence to the fact that statements like the frozen-time character of evolution, as other ontological claims about GR, are model dependent.     

Trends of karyological evolution in Pelobatoid frogs

Summary The Pelobatids have a basal karyotype of 26 chromosomes, with 6 pairs of large and 7 of small chromosomes (6+7). From this karyotype, shown byLeptobrachium, Pelobates andScaphiopus, those ofMegophrys andSpea (5+8) may be derived, as well as that ofL. pelodytoides, with only 24 chromosomes.Pelodytes has 24 chromosomes and a karyotype 7+5 differing from that of typical Pelobatids under several respects.Research supported by a contribution from the Italian C. N. R.     

Occurrence of a blood group A-like substance in eggs of the prosobranch snailPomacea canaliculata

Summary In the eggs of the prosobranch snailsPomacea canaliculata andPomacea insularum a blood group A-like substance has been detected by anti-A from the snailsHelix pomatia, Helix aspersa andCepaea nemoralis.     

Alexis Fontaine's ‘Fluxio-differential method’ and the origins of the calculus of several variables

Alexis Fontaine des Bertins (1704–1771) was the first French mathematician to introduce what we would now regard as results in the calculus of several variables. One example is Fontaine's theorem nF = (?F/?x)x + (?F/?y)y of 1737 for homogeneous expressions F of degree n in x and y. Many years later Fontaine indicated this particular result to have been ‘a continuation of the method of solution’ introduced by him in 1734 to solve the problem of the tautochrones. It is tempting to disregard this announcement, since the method applied to the tautochrones was a method of variations and not manifestly an exercise in the calculus of several variables. Do we have just another case of a mathematician's confusion about the origins of his earlier work? In this paper I describe Fontaine's possible intentions in his remarks.     

On the usefulness of macroeconomic forecasts as inputs to forecasting models

A forecasting model for y_t based on its relationship to exogenous variables (e.g. x?_t) must use x?_t, the forecast of x?_t. An example is given where commercially available x?_t's are sufficiently inaccurate that a univariate model for y_t appears preferable. For a variety of types of models inclusion of an exogenous variable x?_t is shown to worsen the y_t forecasts whenever x?_t must itself be forecast by x?_t and MSE (x?_t) > Var (x?_t). Tests with forecasts from a variety of sources indicate that, with a few notable exceptions, MSE (x?_t) > Var (x?_t) is common for macroeconomic forecasts more than a quarter or two ahead. Thus, either:

• (a) available medium range forecasts for many macroeconomic variables (e.g. the GNP growth rate) are not an improvement over the sample mean (so that such variables are not useful explanatory variables in forecasting models), and/or
• (b) the suboptimization involved in directly replacing x?_t by x?_t is a luxury that we cannot afford.

     

DNG1, a Dictyostelium homologue of tumor suppressor ING1 regulates differentiation of Dictyostelium cells

dng1 is a Dictyostelium homologue of the mammalian tumor suppressor ING gene. DNG1 protein localizes in the nucleus, and has a highly conserved PHD finger domain found in chromatin-remodeling proteins. Both dng1 disruption and overexpression impaired cell proliferation. In dng1-null cells, the progression of differentiation was delayed in a cell-density-dependent manner, and many tiny aggregates were formed. Exogenously applied cAMP pulses reversed the inhibitory effect caused by dng1 disruption on the aggregation during early development, but formation of tiny aggregates was not restored. dng1-overexpressing cells acquired the ability to undergo chemotaxis to cAMP earlier and exhibited enhanced differentiation. These phenotypes were found to be coupled with altered expressions of early genes such as cAMP receptor 1 (car1) and contact site A (csA). Furthermore, disordered histone modifications were demonstrated in dng1-null cells. These results suggest a regulatory role of dng1 in the transition of cells from growth to differentiation.Received 29 December 2004; received after revision 24 May 2005; accepted 26 May 2005     

Robust estimation of conditional variance of time series using density power divergences

Suppose Z _t is the square of a time series Y _t whose conditional mean is zero. We do not specify a model for Y _t , but assume that there exists a p ×1 parameter vector Φ such that the conditional distribution of Z _t | Z _{t ?1} is the same as that of , where Z _{t ?1}=(Z _{t ?1},…,Z _{t ?p} )^T for some lag p ?1. Consequently, the conditional variance of Y _t is some function of . To estimate Φ , we propose a robust estimation methodology based on density power divergences (DPD) indexed by a tuning parameter α ∈[0,1], which yields a continuum of estimators, , where α controls the trade‐off between robustness and efficiency of the DPD estimators. For each α , is shown to be strongly consistent. We develop data‐dependent criteria for the selection of optimal α and lag p in practice. We illustrate the usefulness of our DPD methodology via simulation studies for ARCH‐type models, where the errors are drawn from a gross‐error contamination model and the conditional variance is a linear and/or nonlinear function of . Furthermore, we analyze the Chicago Board Options Exchange Dow Jones volatility index data and show that our DPD approach yields viable models for the conditional variance, which are as good as, or superior to, ARCH/GARCH models and two other divergence‐based models in terms of in‐sample and out‐of‐sample forecasts.     

Mating patterns of virgin and inseminatedDrosophila melanogaster of different alcohol dehydrogenase (Adh) genotypes

Summary Female choice mating experiments showed virgin femaleD. melanogaster of the 3Adh genotypes chose heterozygous (Adh ^F /Adh ^S ) males most commonly. Inseminated females chose mates randomly, but the likelihood of a female remating was genotypically dependent.Acknowledgment. Financial support was provided by the Australian Research Grants Commitee.     

On the specific attraction of the males of the six-toothed spruce bark beetle,Pityogenes chalcographus (L.) to a mixture of synthetic pheromones of the eight-toothed spruce bark beetle,Ips typographus (L.) (Coleoptera,Scolytidae)

Summary Traps containing a mixture of attractants forIps typographus also caught other species of bark beetles. The numbers ofPityogenes chalcographus, Pityogenes conjunctus, andTrypodendron lineatum were too high to be interpreted as accidental captures. The males of the polygamousP. chalcographus were specifically attracted, indicating that theI. typographus pheromone or one of its components acts as a kairomone and primary attractant for the pioneering males ofP. chalcographus.     

The morphology of the lateral line system in 3 species of Pacific cottoid fishes occupying disparate habitats

Summary The superficial neuromasts inLeptocottus armatus (Pacific staghorn sculpin) andOligocottus maculosus (tidepool sculpin) are depressed below the rim of an epidermal collar, whereas the superficial neuromasts inPsychrolutes paradoxus (tadpole sculpin) are surrounded by a pair of hillocks. The lateral line canal system appears identical across these species, whereas the system of superficial neuromasts shows some intraspecific variation. The sand-livingLeptocottus has a more highly developed system of superficial neuromasts thanOligocottus, which lives in a rocky habitat. The present results support the correlation between lateral line system morphology and habitat features previously postulated by Dijkgraaf.     

Systematics and evolution of holarctic Pierinae (Lepidoptera). An enzyme electrophoretic approach

Summary Enzyme electrophoretic data show a remarkably high degree of genetic similarity within the European group ofnapi s.l. whereas genetic differences exist at several loci between the European and the North American taxa ofnapi s.l. It is concluded that the European taxa did not differentiate to the species level and form a phylogenetically young group. The North American taxa included in this study are specifically distinct from Europeannapi and separated much earlier. Within these North American taxamarginalis, oleracea andvirginiensis did undergo speciation. The data show a splitting of the genusPieris into three species groups, each genetically differentiated to the same level. The splitting ofPieris into two genera, as suggested by earlier investigators, is not supported here.     

Cytogenetic studies of Hynobiidae (Urodela). XIV. Analysis of the chromosome of a Chinese salamander, Batrachuperus pinchonii (David)

The chromosome number of a Chinese salamander, Batrachuperus pinchonii, was re-examined. Adults and embryonic specimens had a diploid number of 66, with 33 bivalents during meiosis, in contrast to previous reported results. Furthermore, when C-banding analysis was performed with embryos, chromosomes with banding patterns homoeologous to those of Salamandrella keyserlingii and Hynobius species were found. It appears, therefore, that Batrachuperus, Salamandrella and Hynobius might be derived from a common ancestral species in eastern Asia. Received 22 August 1997; received after revision 14 October 1997; accepted 20 November 1997     

Synthesis of eledoisin

Résumé LeL-pyroglutamyl-L-prolyl-L-séryl--N-carbotert.-butoxy-L-lysyl azide et leL-aspartyl-L-alanyl-L-phénylalanyl-L-isoleucyl-glycyl-L-leucyl-L-méthioninamide ont été synthétisés, puis condensés enL-pyroglutamyl-L-prolyl-L-séryl--N-carbo-tert. butoxy-L-lysyl-L-aspartyl-L-alanyl-L-phénylalanyl-L-isoleucyl-glycyl-L-leucyl-L-méthioninamide. Après traitement par l'acide trifluoroacétique, ce dernier a donné l'endécapeptide,L-pyroglutamyl-L-prolyl-L-séryl-L-lysyl-L-aspartyl-L-analyl-L-phénylalanyl-L-isoleucyl-glycyl-L-leucyl-L-méthioninamide, qui s'est montré doué des mêmes propriétés chimiques, physiques et biologiques que l'élédoisine naturelle.     

Secretion of chitinase byAphanocladium album,a hyperparasite of wheat rust

Summary Aphanocladium album, a hyperparasite of wheat rust,Puccinia graminis vartritici, secretes chitinase when grown on a chitin-containing medium. This enzyme was purified and shown to attack the mycelium of the parasitized rust fungus.Supported, in part, by a grant from the Naef-Roth-Foundation.     

Identification of mucondialdehyde as a novel stress metabolite

In a survey of antifungal stress compounds induced by cupric chloride we found that leaves ofChenopodium album exuded a highly fungitoxic metabolite mucondialdehyde (trans-2,trans-4-hexadienedial), which was associated with 13-oxo-9,11-tridecadienoic acids (cis-9,trans-11 andtrans-9,trans-11 isomers) presumably resulting from -scission of 13-hydroperoxy-octadecadi(tri)enoic acid. The biogenesis and role as a general defensive agent in plants are briefly discussed.     

Effect of volatile phylloplane fungal metabolites on the growth of a foliar pathogen

Summary Volatile fungal metabolites excluded from various leaf-inhabiting microfungi were tested for their mycostatic activity against the growth ofPestalotiopsis funerea Desm., a leaf spot pathogen ofEucalyptus globulus Labill. Findings reveal thatTrichoderma viride andTrichothecium roseum exhibited significant antagonistic action, however,Cladosporium herbarum, Nigrospora sphaerica andPapulaspora sp. showed a stimulating effect. A morphophysiological effect was also observed.Acknowledgments. The grant from CSIR (India Govt.) in the form of SRF and laboratory facility from the Department of Botany, Banaras Hindu University are gratefully acknowledged. Thanks are due to Professor R.S. Dwivedi for guidance and constant encouragements.     

Change in compatibility after microinjection of cytoplasm in amoebae

Summary A cytoplasmic fraction from D32, a clone of amoebae derived fromAmoeba proteus injected with cytoplasm fromA. discoides, inhibited cell division inA. proteus but not inA. discoides indicating a permanent change with respect to compatibility.