Permeability coefficients of the egg-case membrane ofScyliorhinus canicula L.

Summary The cleidoic egg-case of the dogfish appears to have a highly porous and permeable outer membrane, the pore radius being computed to be 13.6 Å. It does not present any physiological barrier to small molecules and therefore constitutes an open ionic and osmotic system for the embryo. Being a porous protein membrane it may be of value as a model for molecular transport mechanisms.     

Response of the ampullae of Lorenzini to static combined electric and thermal stimuli in Scyliorhinus canicula.

The effect of long-lasting electric currents on the Lorenzinian ampullae at constant temperatures between and 25 degrees C was investigated in the dogfish (Scyliorhinus canicula). Steady state neural impulse patterns in single afferent units were analyzed by plotting interval length histograms and computing mean values and standard deviations for currents between -100 and +100 nA. The mean values depended on temperature and on current strength; the relative standard deviations remained almost constant (ca. 20--30%). Negative currents, inserted at the orifice of the ampullary canal led to higher, and positive currents to lower, steady impulse rates in the whole temperature range investigated here. This static component of electrosensitivity again disappeared at higher currents (of 50 nA and more; electric overstimulation). The maximum static response was two orders of magnitude less than the maximum dynamic component of electroreception. The electrosensitivity depended on temperature: the ampullae were most sensitive to electric currents between 13 and 19 degrees C. The maximal neural activity at 19 degrees C was not shifted to higher or lower temperatures by electric stimulation. A constant equivalent of electric and thermal stimulation throughout the tested temperature and current range could not be found.     

Cutaneous respiration in a Selachian (Scyliorhinus caniculus L.)]

Cutaneous oxygen consumption (Mo2) and cutaneous oxygen uptake from the external medium were investigated in an elasmobranch Scyliorhinus caniculus L. In spite of an important Mo2 (2.75 +/- 0.28 nmol.mn-1 cm-2 ; n=6) a positive net flux of oxygen through the skin takes place (2.98 +/- 0.33 nmol. mn-1 .cm-2). The skin of the dogfish in an exchanger for oxygen supplying 10% of oxygen requirements of other organs of the body in these experimental conditions (t = 13 degrees C).     

Permeability of the isolated human amniotic membrane to divalent cations]

Membrane potential and resistance recordings, in vitro, show that Mg++ does not pass through the amnion from the inside of the amniotic compartment to the outside of the amniotic membrane. Mg++ may become fixed on the surface or in the midst of the amniotic membrane. However, Mg++ diffuses in the opposite direction. Ca++, Ba++, Sr++ diffuse in both directions across the amniotic membrane.     

Permeability of arthrodial membrane to water: A first measurement using in vivo techniques

Summary Despite differences in surface morphology and fine structure, the permeabilities of untreated scorpion sternite and pleural cuticle to water are similar (0.69 versus 0.79 g·cm^–2·h^–1·mm Hg^–1). Hexane applied to pleural membrane increased its permeability 9-fold, but neither hexane nor chloroform: methanol had much effect on sternite permeability. When sternite cuticle was treated with 1.0 N KOH followed by chloroform: methanol, permeability increased about three times over control values. In contrast, cockroach pronotum, which is 17 times more permeable than scorpion sternite, exhibited a marked increase in permeability when treated with just hexane. In both the scorpion pleuron and cockroach pronotum, disruption of the lipid barrier caused by rubbing is partially responsible for the higher permeabilities observed following solvent treatment.     

高瓦斯低透气性煤层水力压裂增透技术研究

针对高瓦斯低透气性煤层瓦斯抽采效率低、钻孔施工量大等问题,结合高压水动力特性,提出水力压裂技术来提高煤层透气性。分析了水力压裂煤体致裂增透机理,提出起裂压力的临界值公式;计算得出水力压裂参数,选用合理的压裂设备和工艺。分析了压裂半径、压裂后煤层透气性及抽采效果等,现场应用结果表明:水力压裂使区域内煤层透气性提高了67倍,在煤层走向上的影响半径可达到20~24 m,能有效增加钻孔的透气性,煤层瓦斯抽采浓度、纯量均提高4倍以上,抽采浓度衰减时间明显增长,有效提高了瓦斯抽采效率。     

Electrophysiological characteristics ofBombyx mori L. ventral nerve cord (effect of sodium and potassium on the membrane potential)

Summary Na⁺ and K⁺ effects on the resting cellular membrane potential of desheathed ganglia of theBombyx mori L. ventral nerve cord have been studied. The cells are depolarized by high concentrations of external potassium ions in the same way as in vertebrates, mollusca and crustacean cells. The possibility that the behaviour of the resting potential is not only influenced by the potassium equilibrium potential, but also by the conductances to other ions, is discussed.The authors are indebted to Prof V. Capraro, Drs B. Giordana and F. Sacchi for helpful comments and criticism.     

Permeability of Ehrlich ascites cells and spectrophotometric assay of the sub-cellular enzymes

Summary A permeabilization method which allows the assay of intracellular enzymes of the Ehrlich ascites cells is described. The developmental changes in the activity of lactic dehydrogenase and glucose-6-phosphate dehydrogenase of toluene-treated Ehrlich ascites cells were studied.Acknowledgment. M.D. is grateful to CSIR for the award of Junior fellowship.     

Permeability of rat heart myocytes to cytochrome c

Rat heart myocytes undergoing progressive damage demonstrate morphological changes of shortening and swelling followed by the formation of intracellular vacuoles and plasma membrane blebbing. The damaged myocytes displayed impaired N,N'-tetramethyl-p-phenyldiamine (TMPD) ascorbate-stimulated respiratory activity which was restored by the addition of reduced cytochrome c to the cell culture medium. To clarify the role played by cytochrome c in the impairment of cell respiration, polarographic, spectrophotometric and fluorescence as well as electron microscopy imaging experiments were performed. TMPD/ascorbate-stimulated respiratory activity returned to control levels, at approximately 20 microM cytochrome c, establishing the threshold below which the turnover rate by cytochrome c oxidase in the cell depends on cytochrome concentration. Mildly damaged cardiac myocytes, as indicated by cell shortening, retention of visible striations and free-fluorescein exclusion, together with the absence of lactate dehydrogenase leakage and exclusion of trypan blue, were able to oxidize exogenous cytochrome c and were permeable to fluorescein-conjugated cytochrome c. The results, while consistent with an early cytochrome c release observed at the beginning of cell death, elucidate the role played by cytochrome c in the kinetic control of mitochondrial electron transfer under pathological conditions, particularly those involving the terminal part of the respiratory chain. These data are the first to demonstrate that the sarcolemma of cardiac myocytes, damaged but still viable, is permeable to cytochrome c.     

Permeability of the golden hamster placenta to inorganic lead: Radioautographic evidence

Zusammenfassung Trächtigen Goldhamstern wurde am 7. und 8. Tag²¹⁰Pb intravenös verabericht. Von den Placentamembranen und vom Embryo wurde radioaktives Metallion stark aufgenommen und die Einglagerung autoradiographisch verfolgt.     

Electron microscope observation of "thyroidectomy cells" in the hypophyseal ventral lobe of a Chondrichthyan fish, the small spotted dogfish, Scyllium canicula Cuv.]

In the adenohypophysis of very young spotted dogfish, only one kind of granulated cells show strcutural changes following thyroidectomy: the small granule cells of the ventral lobe (granule diameter about 110 nm). These cells are similar to the thyrotrophs of other Vertebrates, and well distinct from the supposed gonadotrophs found in the same lobe.     

Permeability of cellophane membranes to parotid proteins during dialysis

Summary Pooled parotid saliva was dialyzed in cellophane membranes against water for periods of up to 1 week and loss of proteins was monitored by acrylamide gel-electrophoresis. A gradual loss of cationic proteins was observed whereas anionic proteins were not appreciably affected. Loss of the cationic proteins could be greatly reduced by performing dialyses against dilute electrolyte solutions rather than water. These effects were attributed primarily to electrostatic charges associated with the dialysis membranes.The assistance of Mr E. Pederson and Dr G. Clark is gratefully acknowledged. Supported by Naval Medical Research and Development Command Project MR005.19 6048, Bethesda, Maryland. The opinions expressed herein are those of the authors and are not be construed as reflecting the views of the Navy Department or the Naval Service at large. The use of commercially available products does not imply endorsement of these products or preference to other similar products on the market.Deceased.