RP-HPLC法测定复方丹参胶囊中丹参素的含量

为测定复方丹参胶囊中丹参素含量,采用反相高效液相色谱法,D ikm a HPLC column色谱柱(5μm,200×4.6 mm),以乙腈—水—冰醋酸(5∶160∶1)为流动相,流速1.0 mL/m in,检测波长280nm,柱温30℃测定.RP-HPLC测定的线性范围为0.28~1.40μg,相关系数为r=0.9998;胶囊中丹参素的平均回收率为100.17%,RSD=0.78%(n=6),精密度良好(RSD=0.64%).该法操作简便、快速、准确,适用于复方丹参胶囊中丹参素的含量测定.     

HPLC-ELSD测定强心胶囊中黄芪甲苷质量比

建立强心胶囊中黄芪甲苷的质量比测定方法.色谱柱VP-ODS(4.6 mm×150 mm.5μm)流动相为乙腈-水(34∶66),流速为1.0 mL/min,柱温为35℃,蒸发光散射检测器漂移管温度105℃;氮气流速为2.5 L/min,黄芪甲苷在2.6~13.0μg呈良好的线性关系,r=0.999 5,平均回收率为98.83%,RSD=0.47%.本法方便准确,重复性好,灵敏度高.可用于强心胶囊中黄芪甲苷的质量比测定.     

正交试验法优选通脉胶囊提取工艺

优选通脉胶囊的最佳提取工艺,以提取液中含黄芪甲苷的量和出膏率为评价指标,采用正交试验法设计并优化了通脉胶囊最佳提取工艺.黄芪甲苷测定的色谱条件为：Diamonsil C₁₈色谱柱(内径4.6 mm,柱长25 cm,粒度5μm);采用水∶乙腈(68∶32)为流动相;流速：1.0 mL/min;蒸发光散射检测器检测条件：空气气体流速2.5 L/min,漂移管温度105℃.实验结果表明,通脉胶囊的最佳提取工艺为12倍量水,提取2次,第一次2 h,第二次1.5 h.本实验确定的工艺合理可行,为通脉胶囊提取工艺的确定提供了实验依据.     

HPLC法测定黄豆苷元胶囊的含量

建立HPLC法测定黄豆苷元胶囊的含量.方法:采用Hypersil ODS 色谱柱,(4.6 mm ×150 mm,5 μm),流动相水-乙腈(70∶30),流速为1 mL·min-1,检测波长249 nm,柱温30 ℃,进样量15 μL.结果:在1.0～20.0 μg·min-1浓度范围内线性关系良好,方法回收率为101 98%,RSD为1.45% (n＝9),精密度良好.结论:本方法灵敏、准确、专属性强,用于该制剂含量测定结果满意.     

高效液相色谱法测定消化性溃疡治疗药物兰索拉唑

目的:建立并优化测定兰索拉唑胶囊中兰索拉唑含量的高效液相色谱法.方法:色谱柱为Gemini C18(4.6 mm×250 mm,5μm),流动相:乙腈:0.1mol·L~(-1)磷酸盐缓冲液(pH调至7.0)(70:30,V/V),检测波长为284nm.结果:兰索拉唑的线性范围浓度为10~200mg·L~(-1),相关系数为0.9996,平均加样回收率为96.1%,RSD=1.65%.结论:该方法操作简单、快速、准确,可用于兰索拉唑胶囊中兰索拉唑的含量测定.     

不同提取方法对止咳宁嗽胶囊中活性成分含量的影响

利用HPLC研究不同提取方法(超声法、浸泡法和索式提取法)下止咳宁嗽胶囊中绿原酸和橙皮苷的含量.色谱柱:TC-C18(4. 6 mm×250 mm×5μm);柱温:25℃;进样量10μL;流动相:A为乙腈,B为0. 2%冰乙酸,梯度洗脱(0～15 min:10%～30%A,15～30 min:30%～85%A,30～40 min:85%～85%A,40～48 min:85%～10%A);流速:1. 00 m L·min-1;测定波长:327 nm(0～10 min),280 nm(10～48 min).优化色谱条件下,可以得到分离状况较好的色谱峰,对止咳宁嗽胶囊采用索氏提取法,测得其中的橙皮苷和绿原酸的含量最高.     

加替沙星的紫外、荧光分光光度法和高效液相色谱法测定

采用紫外分光光度法、荧光分光光度法和反相高效液相色谱法测定胶囊中加替沙星含量. 在0.04 mol·L-1 HAc-NaAc缓冲溶液(pH=4.3)中直接根据加替沙星的紫外吸收光谱进行测定;于0.04 mol·L-1 HAc-NaAc缓冲溶液(pH=4.3)和0.014 mol·L-1十二烷基磺酸钠(SDS)体系中,以330 nm作为激发波长,在484 nm处测定其荧光强度;以VP-ODS为色谱柱(150 mm×4.6 mm, 5.0 μm), 柱温为35 ℃,流动相为V(CH3OH)∶V(H2O)=45∶55 (用醋酸调节pH至3.5), 流速为1.0 mL·min-1, 检测波长为292 nm,建立了测定加替沙星的反相高效液相色谱法. 3种方法对胶囊中加替沙星含量5次测定结果的平均值分别为98%,96%,102%, RSD分别为2.5%,2.7%,1.9%, 表明该方法可用于加替沙星制剂的质量控制.     

HPLC-UV法测定不同产地知母中芒果苷的含量

考察不同产地知母中芒果苷的含量.采用高效液相色谱法,测定了12个产地知母中芒果苷的含量.色谱柱为迪马公司DiamonsilR(钻石)C18柱(250 mm×4.6 mm,5μm);检测波长为254 nm;温度为20℃;流动相为乙腈-水(15∶85体积比),(磷酸调pH=3.0);流速为1 mL/min.产自山西天镇的知母中芒果苷含量最高.本法测定知母中芒果苷含量简便、快速、准确,重现性好.     

HPLC法测定调经养颜胶囊中人参皂苷Rg1的含量

目的:建立高效液相色谱法测定调经养颜胶囊中人参皂苷Rg1的含量。方法:用Hypersil BDS C18(5μm4.6×250mm)色谱柱,乙腈-0.05%磷酸溶液(21:79)为流动相,流速1.0mL/min;检测波长:203nm。结果:人参皂苷Rg1的进样量在3.012μg～7.028μg范围内线性关系良好(r=0.9997),平均加样回收率为99.22%(RSD=0.98%),符合分析要求。结论:对样品的测定条件进行了优选,为调经养颜胶囊中人参皂苷Rg1的测定提供了一种准确可靠的HPLC方法。     

RP-HPLC法测定木香与川木香中木香烃内酯的含量

目的:测定木香与川木香中木香烃内酯的含量.方法:采用RP-HPLC法,用kromasil色谱柱(250mm×4.6mm,5μm),以甲醇-水梯度洗脱,流速1ml·min-1,柱温30℃,检测波长225nm.结果:在0.4～1.4μg之间,峰面积与进样量的线性关系为A=671028.2864C-65332.7036(r=0.9999),平均回收率为97.3%.结论:所建方法简便、准确、重复性好,可用于木香与川木香中木香烃内酯的含量测定.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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