Cell division

In creating the mitotic spindle and the contractile ring, natural selection has engineered fascinating precision machines whose movements depend upon forces generated by ensembles of cytoskeletal proteins. These machines segregate chromosomes and divide the cell with high fidelity. Current research on the mechanisms and regulation of spindle morphogenesis, chromosome motility and cytokinesis emphasizes how ensembles of dynamic cytoskeletal polymers and multiple motors cooperate to generate the forces that guide the cell through mitosis and cytokinesis.     

6个小鼠ES细胞系的建立和鉴定

报道从小鼠129/ter品系中建成6个胚胎干细胞系,经过核型检查,体内、体外分化等鉴定后发现,6个细胞系中有2个细胞系是正常二倍体,其中ES细胞系MESPU 13为XY型,其核骨架蛋白质成分相当于3～4d(天)的鼠胚细胞的核骨架成分,可长期维持二倍体未分化状态,并具有高效的嵌合能力。核型不正常的细胞系所形成的畸胎瘤生长较快,但细胞的分化程度与核型正常的ES细胞有所不同。对影响建系结果的各种因素进行了讨论。