HPLC法测定鲜天麻中多指标成分的含量

建立了高效液相色谱测定鲜天麻中天麻素、对羟基苯甲醇、对羟基苯甲醛、腺苷、巴利森苷A、4,4'-二羟基二苄基醚6种成分含量的方法。采用Agilent 1120高效液相系统,YMC-PEAK ODS-A column(250 mm×4.6 mm,5μm)色谱柱,流动相为0.1%甲酸水溶液(A)-甲醇溶液(B),梯度洗脱:0~5 min,5%B;5~65 min,5%~40%B;65~80 min,40%~100%B;分析时间80 mins;流速1 m L/min;柱温25℃;进样量50μL。其中天麻素、对羟基苯甲醇、对羟基苯甲醛、腺苷、巴利森苷A、4,4'-二羟基二苄基醚6种成分在线性范围内均具有良好的线性关系(R≥0.999 2);平均回收率在94.20%~99.68%,相对标准偏差小于1.96%。在不同品种的鲜天麻中,6种成分的量存在差异,红天麻各成分含量稍高于乌天麻。同一品种天麻中,巴利森苷类成分含量较高,腺苷和4,4'-二羟基二苄基醚含量均较低,且鲜天麻中提取的天麻素等6种成分在8 h内稳定。该方法分离效果与重复性好、快速、简便,能够为客观、全面地研究天麻药材提供参考。     

一测多评法测定壮药三加皮中5个酚酸类成分的含量

为建立一种可同时测定三加皮中5种酚酸类成分（新绿原酸、绿原酸、异绿原酸B、异绿原酸A、异绿原酸C）含量的一测多评法（QAMS）,以绿原酸为内参物,采用色谱柱phenomenex C₁₈（4.6 mm×250 mm,5 μL）,以乙腈（C）-0.2%磷酸（A）为流动相进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长325 nm。建立新绿原酸、异绿原酸B、异绿原酸A、异绿原酸C与内参物绿原酸的相对校正因子,计算待测成分的含量,与外标法（ESM）测定的结果比较,评价QAMS的可行性与准确性。结果显示,所建立的三加皮中新绿原酸、绿原酸、异绿原酸B、异绿原酸A、异绿原酸C的相对校正因子重现性良好;10个产地的三加皮中5种成分QAMS计算值与ESM实测值比较无显著差异。本研究建立的一测多评法（QAMS）可应用于三加皮中5种酚酸类成分含量测定。     

普洱茶渥堆发酵中活性成分测定及其相关性分析

利用高效液相色谱法分别测定表没食子儿茶素没食子酸酯、表儿茶素、茶氨酸、槲皮素、黄嘌呤和次黄嘌呤在普洱茶发酵过程中的含量变化,发现在发酵过程中,表没食子儿茶素没食子酸酯、表儿茶素、茶氨酸和槲皮素的含量下降,黄嘌呤和次黄嘌呤的含量上升。对这6种成分进行了相关性分析,发现多酚类与氨基酸类呈极显著正相关。     

HPLC法测定蒙药复方述达格-4中1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮的含量

建立测定蒙药复方述达格-4药材中1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮含量的方法.色谱柱为InertsilODS-3(4.6mm×250mm,5μm),以乙腈(B)-0.5%磷酸水溶液(A)为流动相,梯度洗脱(0~20min,0%B→45%B;20~25min,45%B→55%B;25~40min,55%B→100%B),流速1.0ml·min-1,检测波长为210nm,柱温30℃,进样量20μL.结果表明蒙药复方述达格-4药材中1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮在0.102-1.02μg(r=0.99999)质量范围内与色谱峰面积呈良好的线性关系,精密度、稳定性、重复性试验的RSD≤2%,平均加样回收率(n=6)为99.63%(RSD=1.77%).本法简便、快速、准确,可为蒙药复方述达格-4的质量控制提供依据.     

玉龙乌头的化学成分研究

运用色谱分离材料和手段,结合现代波谱分析技术,从乌头属植物玉龙乌头中首次分离鉴定了12个非生物碱成分:4-hydroxy-4-(5-(hydroxymethyl)furan-2-yl)butan-2-one(1),(E)-4-[5-(hydroxymethyl)furan-2-yl]but-3-en-2-one(2),5-羟甲基糠醛(3),E-3-carboxy-2-petene-dioate 5-methyl ester(4),Z-3-carboxy-2-petenedioate 5-methyl ester(5),对羟基反式肉桂酸(6),对羟基顺式肉桂酸(7),藜芦酸甲酯(8),环氧松柏醇(9),酪醇(10),连翘脂素(11)和β-sitosteryl-3-O-β-D-glucopyranoside-2'-O-palmitate(12).其中化合物1是一个新天然产物,其数据经2D NMR进行了归属并首次报道.     

8-羟基喹啉合成条件的研究及其应用

以邻硝基苯酚、邻氨基苯酚、甘油为原料,在浓硫酸催化下,用Skraup法合成了8-羟基喹啉;实验结果表明,其最佳反应条件为：邻硝基苯酚与邻氨基苯酚物质量的比为1∶2,反应温度为130℃,反应时间为2.5 h;用8-羟基喹啉荧光分光光度法测定油炸食品中铝的含量,为食品加工和安全检测提供了重要参考。     

正交试验法优化合成8-羟基喹啉及应用研究

采用Skraup法催化合成8-羟基喹啉,通过正交试验法研究了反应物物质的量比、催化剂用量、反应时间、反应温度对其产率的影响,确定了其最佳合成条件:n邻硝基苯酚∶n邻氨基苯酚=1.2∶2,催化剂浓硫酸用量为4.5 m L,反应温度为145℃,反应时间为2 h,8-羟基喹啉的产率为56.4%。用8-羟基喹啉荧光分光光度法测定油炸食品中铝的含量,该方法操作简单,测定准确,适合质检部门测定油炸食品中铝的含量。     

铜与8-羟基喹啉配合物的电化学研究

利用循环伏安法研究了铜与8-羟基喹啉形成配合物的氧化还原性质和配合反应机理。讨论了不同配比配合物的配位情况,得到以各种不同配比下的铜与8-羟基喹啉电化学性质的关系曲线。在优化的实验条件下;pH=5.2(Na2HPO4-柠檬酸),静止时间80 s,吸附时间3 min,扫速0.05 V/s,铜与8-羟基喹啉形成的配合物具有一定的电化学活性,主要为1∶1配合。在0.154 V和0.60 V附近出现2个氧化峰,当配体量较小时,0.154 V处生成铜(Ⅰ)-8-羟基喹啉络合物,平衡常数为869,为表面控制。随着配体量的增大,在0.6V处出现铜(Ⅱ)-8-羟基喹啉络合物的氧化峰,平衡常数为2.41×107,为表面控制。     

HPLC同时测定广地龙中5种核苷类成分含量

建立同时测定广地龙中5种核苷类成分含量的HPLC法。采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(5∶95,体积比)等度洗脱,流速1.0 mL/min,检测波长254 nm,柱温30℃,测定了广地龙药材中5种核苷类成分的含量。结果表明:尿嘧啶、次黄嘌呤、尿苷、肌苷、鸟苷分别在14.84～111.30 ng(r=0.999 9)、126.00～945.00 ng(r=0.999 8)、63.60～477.00 ng(r=0.999 9)、612.00～4 590.00 ng(r=0.999 8)、41.60～312.00 ng(r=0.999 9)线性关系良好,平均加样回收率(n=9)分别为99.27%、99.14%、98.59%、98.69%、99.91%,RSD(n=9)分别为0.95%、0.86%、1.01%、0.86%、1.91%;20批广地龙中尿嘧啶、次黄嘌呤、尿苷、肌苷、鸟苷含量分别为0.051 0～0.111 0、0.243 1～0.943 6、0.171 9～0.508 0、1.559 6～3.861 1、0.165 5～0.250 1 mg/g,5种总核苷含量为3.309 9～4.565 9 mg/g。该方法简便、可靠、重复性好、精密度高,可用于同时测定广地龙中5种核苷类成分的含量。     

指纹图谱结合一测多评法测定金银花叶的化学成分

为开发利用金银花叶资源,采用HPLC-DAD方法建立金银花叶HPLC指纹图谱,并用一测多评法（QAMS）和外标法相结合的质量评价模式,测定一年12个月不同时期内金银花叶中12种成分的含量,验证此方法测定金银花叶成分的可行性和准确性.结果显示,12个月中金银花叶的HPLC指纹图谱相似度大于0.925;QAMS法测得的金银花叶中12种成分含量与外标法测定结果相比较,两组数据无显著性差异（P>0.05）.由此说明,指纹图谱与QAMS相结合评价金银花叶质量的方法简便、准确度高、重复性好,可为金银花叶的质量评价提供参考,为金银花叶的进一步开发利用提供依据.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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