Differences in in vitro incorporation of tritiated deoxycytidine and tritiated thymidine into human lymphocytes.

Human lymphocytes stimulated in vitro by PHA or PWM incorporate 3H-CdR into DNA, but less well than 3H-TdR. More 3H-CdR is incorporated in populations enriched for T cells. No 3H-CdR is used by cells stimulated by lipopolysaccharides under circumstances in which 3H-TdR is incorporated. We conclude that human T cells and B cells differ in their ability to use 3H-CdR.     

Differences in utilization of tritiated thymidine and tritiated deoxycytidine by rat lymph node cells

Summary Large lymphoid cells and plasma cells in antigenically stimulated rat lymph nodes retain less label after injection of³H-CdR than after injection of³H-TdR. There is no difference amongst small lymphocytes. The data are consistent with a defect in the utilization of³H-CdR in the late stages of B cell maturation in the rat.     

Differences in utilization of tritiated thymidine and tritiated deoxycytidine by rat lymph node cells.

Large lymphoid cells and plasma cells in antigenically stimulated rat lymph nodes retain less label after injection of 3H-CdR than after injection of 3H-TdR. There is no difference amongst small lymphocytes. The data are consistent with a defect in the utilization of 3H-CdR in the late stages of B cell maturation in the rat.     

Effect of folate and folinate on3H-thymidine incorporation by transforming human lymphocytes in vitro

Summary The incorporation of³H-thymidine by Concanavalin A-stimulated human peripheral blood lymphocytes in vitro is inhibited by the presence of folate or folinate in the culture medium in a concentration-dependent manner.     

Development of the gonads of mice after intense incorporation of tritiated thymidine during the period of oogenesis

Résumé Le développement des gonades de souris après une forte incorporation de thymidine-³H, administrée pendant la période de l'oogénèse, a été étudié. Suite à ce traitement les souris femelles sont devenues stériles à l'âge de la puberté et le volume de leurs ovaires, dépourvues de cellules germinales, a fortement diminué. Les mâles traités de la même façon ne semblent pas être stérilisés malgré une diminution du volume de leurs testicules.

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This work was supported by a grant from the Fonds de la Recherche scientifique fondamentale collective.     

Stability of tritiated thymidine during prolonged labelling of human blood leukocyte cultures

Zusammenfassung Der Stoffwechsel des³H-Thymidins in Blut-Leukozyten wurde während langfristiger Inkubation studiert. Die Bedingungen waren dieselben, die gewöhnlich for Chromosomenpräparate angewendet werden. So gelang es, menschliche Leukocyten während 72 Stunden mit H³-markiertem Thymidin zu inkubieren, ohne dass das markierte Thymidin durch die Leukozyt-Thymidin-Phosphorylase zerstört wurde.

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This work was supported by research grant No. AM 14529 from the U.S. Public Health Service. D.M. is a Trainee in Genetics (HE 05307) and R.P.C. is a Career Scientist of the Health Research Council of the City of New York.