数据复制技术在质量监督管理系统中的应用

介绍了分布式数据库的复制技术,利用数据复制技术实现了质量监督管理系统中的相关数据的复制．给出了数据复制的基本步骤。     

分布式数据库数据复制技术的研究

对数据复制技术进行了介绍,并分析了这一技术在分布式数据库系统中的具体应用.在分布式数据库系统中通过应用数据复制技术,使得分布式数据库系统的性能和容错性得到明显地提高.本文重点介绍了oracle数据复制技术在国家教育机关中的应用,并给出了数据复制的具体步骤.     

数据复制在装备保障指控系统中的应用与实现

简要介绍了SQLServer2000数据复制技术,并结合装备保障指控系统的实际情况,给出了数据复制在系统中的设计方案。     

数据复制在装备保障指控系统中的应用与实习

简要介绍了SQL Server2000数据复制技术,并结合装备保障指控系统的实际情况,给出了数据复制在系统中的设计方案。     

Sybase复制技术研究及其应用

论述了数据库复制技术的基本概念,重点介绍了 Ｓｙｂａｓｅ 数据复制的结构和实现方法,并结合实例说明复制技术在分布式环境下的应用．     

Sybase复制技术研究及其应用

论述了数据库复制技术的基本概念,重点介绍了Ｓｙｂａｓｅ数据复制的结构和实现方法,并结合实例说明复制技术在分布式环境下的应用。     

Oracle 9i数据复制技术在教务系统中的应用

Oracle 9i针对分布式计算的需要,提供了一套功能强大的数据复制解决方案。本文概述了Oracle数据复制的分类、Oracle高级复制的发展及应用。介绍了Oracle 9i高级复制技术中常用的基本概念、特点,分析了各种复制技术的优缺点,最后确定了一个完整的复制方案,并应用到实际中。     

异构数据库间的数据复制技术及其应用

介绍了数据复制技术 ,针对实际应用构建了数据仓库的DB—ODS—DW三层体系结构 ,并实现了异构数据库之间的数据复制 .     

基于ArcGISEngine的高速公路车辆轨迹查询与回放系统的设计

针对高速公路全程监控系统中车辆流水数据的多源异构特点,提出了采用同步复制技术将各监控子系统的车辆流水数据汇总至监控中心,利用数据同步复制技术形成车辆完整轨迹数据库.采用C#开发语言,结合ArcGIS Engine技术,开发出基于VS 2012平台的车辆轨迹查询与回放系统,实现了实时查询车辆的行车路线,并在地图上动态回放各个时段的车辆运行轨迹.经实际项目测试验证了该系统在交通管理中有很好的实用性.     

分布式数据库的同步复制技术研究

分布式数据库是分布式技术与数据库技术的结合,同步复制技术可以实现分布式数据库系统中全局数据的完整性和一致性。     

PC机与Pocket PC数据同步技术研究

分析了SQL Server Mobile合并复制的模型,介绍了开发环境的搭建,在此基础上实现了.Net Compact Frame Work下合并复制的C#代码。最后测试了合并复制代码,测试结果显示Pocket PC与远程SQL Server 2005保持数据同步。     

数据网格中基于效益函数的副本管理策略

通过分析数据网格中几种经典的副本管理策略的特点,针对网格这样一个协作计算的环境,提出了基于效益函数的副本管理策略,构建具有协作涵义的效益函数作为网格节点替换本地数据副本的依据.在网格模拟器OptorSim上进行的模拟实验结果表明:提出的基于效益函数的策略相比于基于经济模型的副本管理策略,在降低网络的利用率、减少带宽和存储资源消耗的同时缩短了系统的响应时间,达到了提高系统性能的目的.论证了该策略对于副本的管理是行之有效的.     

Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin

Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase alpha-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase delta complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase delta complex. A model for polymerase switching during initiation of DNA replication is presented.     

Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast

Cyclin-dependent kinases (CDKs) drive major cell cycle events including the initiation of chromosomal DNA replication. We identified two S phase CDK (S-CDK) phosphorylation sites in the budding yeast Sld3 protein that, together, are essential for DNA replication. Here we show that, when phosphorylated, these sites bind to the amino-terminal BRCT repeats of Dpb11. An Sld3-Dpb11 fusion construct bypasses the requirement for both Sld3 phosphorylation and the N-terminal BRCT repeats of Dpb11. Co-expression of this fusion with a phospho-mimicking mutant in a second essential CDK substrate, Sld2, promotes DNA replication in the absence of S-CDK. Therefore, Sld2 and Sld3 are the minimal set of S-CDK targets required for DNA replication. DNA replication in cells lacking G1 phase CDK (G1-CDK) required expression of the Cdc7 kinase regulatory subunit, Dbf4, as well as Sld2 and Sld3 bypass. Our results help to explain how G1- and S-CDKs promote DNA replication in yeast.     

Isolation and characterisation of a yeast chromosomal replicator.

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.     

Role for a Drosophila Myb-containing protein complex in site-specific DNA replication

There is considerable interest in the developmental, temporal and tissue-specific patterns of DNA replication in metazoans. Site-specific DNA replication at the chorion loci in Drosophila follicle cells leads to extensive gene amplification, and the organization of the cis-acting DNA elements that regulate this process may provide a model for how such regulation is achieved. Two elements important for amplification of the third chromosome chorion gene cluster, ACE3 and Ori-beta, are directly bound by Orc (origin recognition complex), and two-dimensional gel analysis has revealed that the primary origin used is Ori-beta (refs 7-9). Here we show that the Drosophila homologue of the Myb (Myeloblastosis) oncoprotein family is tightly associated with four additional proteins, and that the complex binds site-specifically to these regulatory DNA elements. Drosophila Myb is required in trans for gene amplification, showing that a Myb protein is directly involved in DNA replication. A Drosophila Myb binding site, as well as the binding site for another Myb complex member (p120), is necessary in cis for replication of reporter transgenes. Chromatin immunoprecipitation experiments localize both proteins to the chorion loci in vivo. These data provide evidence that specific protein complexes bound to replication enhancer elements work together with the general replication machinery for site-specific origin utilization during replication.     

Localization of p53, retinoblastoma and host replication proteins at sites of viral replication in herpes-infected cells.

Replication of DNA occurs at discrete sites in eukaryotic cell nuclei, where replication proteins are clustered into large complexes, or 'replicases'. Similarly, viral DNA replication is a highly structured process, notably in herpes simplex virus type-1 (HSV-1; reviewed in ref. 4) in which large globular 'replication compartments' containing the viral replication machinery exist. Replicating cellular DNA redistributes to these compartments upon HSV-1 infection. We have now used antibodies raised against several cellular proteins to detect changes in their subnuclear localization on HSV-1 infection. We found that various proteins involved in cellular DNA replication move to sites of viral DNA synthesis, whereas a selection of non-replication proteins do not. The retinoblastoma protein and p53 (the products of two putative anti-oncogenes) relocate to the same sites as known DNA replication proteins, suggesting that they may be associated with DNA replication complexes in normal, uninfected cells.     

Break-induced replication and telomerase-independent telomere maintenance require Pol32

Break-induced replication (BIR) is an efficient homologous recombination process to initiate DNA replication when only one end of a chromosome double-strand break shares homology with a template. BIR is thought to re-establish replication at stalled and broken replication forks and to act at eroding telomeres in cells that lack telomerase in pathways known as 'alternative lengthening of telomeres' (reviewed in refs 2, 6). Here we show that, in haploid budding yeast, Rad51-dependent BIR induced by HO endonuclease requires the lagging strand DNA Polalpha-primase complex as well as Poldelta to initiate new DNA synthesis. Polepsilon is not required for the initial primer extension step of BIR but is required to complete 30 kb of new DNA synthesis. Initiation of BIR also requires the nonessential DNA Poldelta subunit Pol32 primarily through its interaction with another Poldelta subunit, Pol31. HO-induced gene conversion, in which both ends of a double-strand break engage in homologous recombination, does not require Pol32. Pol32 is also required for the recovery of both Rad51-dependent and Rad51-independent survivors in yeast strains lacking telomerase. These results strongly suggest that both types of telomere maintenance pathways occur by recombination-dependent DNA replication. Thus Pol32, dispensable for replication and for gene conversion, is uniquely required for BIR; this finding provides an opening into understanding how DNA replication re-start mechanisms operate in eukaryotes. We also note that Pol32 homologues have been identified both in fission yeast and in metazoans where telomerase-independent survivors with alternative telomere maintenance have also been identified.     

CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast

In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes.