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1.
A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin 总被引:76,自引:0,他引:76
T Serafini G Stenbeck A Brecht F Lottspeich L Orci J E Rothman F T Wieland 《Nature》1991,349(6306):215-220
Four high-molecular-weight proteins form the main subunits of the coat of Golgi-derived (non-clathrin) coated vesicles. One of these coat proteins, beta-COP, is identical to a Golgi-associated protein of relative mass 110,000 (110K) that shares homology with the adaptin proteins of clathrin-coated vesicles. This connection, and the comparable molecular weights of the coat proteins of Golgi-derived and clathrin-coated vesicles, indicates that they may be structurally related. The identification of beta-COP as the 110K protein explains the blocking of secretion by the drug brefeldin A. 相似文献
2.
Endothelial vesicles and protein transport 总被引:1,自引:0,他引:1
W E Stehbens 《Nature》1965,207(993):197-198
3.
Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport 总被引:53,自引:0,他引:53
In reconstitution studies N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits both fusion of endocytic vesicles and vesicular transport in the Golgi apparatus. We show here that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicle fusion. Thus, it is likely that a common mechanism for vesicle fusion exists for both the secretory and endocytic pathways of eukaryotic cells. 相似文献
4.
SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer. 总被引:30,自引:0,他引:30
Non-clathrin coated vesicles have been implicated in early steps of intercompartmental transport. A distinct set of coat proteins are peripherally associated with the exterior of purified mammalian intra-Golgi transport vesicles. The 'coatomer', a cytosolic complex containing a similar subunit composition to and sharing at least one subunit (beta-COP) with the coat found on vesicles, has been postulated to be the precursor of this non-clathrin coat. Here we describe the characterization of SEC21, an essential gene required for protein transport from the endoplasmic reticulum to the Golgi in the yeast Saccharomyces cerevisiae. The 105K product of this gene, Sec21p, participates in a cytosolic complex that we show to be a yeast homologue of the mammalian coatomer. These observations demonstrate that a non-clathrin coat protein plays an essential role in intercompartmental transport. 相似文献
5.
Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein 总被引:55,自引:0,他引:55
An N-ethylmaleimide-sensitive fusion protein (NSF) has been purified on the basis of its ability to catalyse vesicular transport within the Golgi stack. We report here that this same protein is required for transport from the endoplasmic reticulum to the Golgi stack in semi-intact cells. This transport process is inhibited by a monoclonal antibody against NSF. Furthermore, pretreatment of semi-intact cells with N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits transport. Addition of highly purified NSF largely restores transport from endoplasmic reticulum to Golgi. These results suggest that NSF is a general component of the transport machinery required for membrane fusion at multiple stages of the secretory pathway. 相似文献
6.
Cytosolic dihydrofolate reductase from mouse contains a cryptic mitochondrial targeting sequence. If this sequence is attached to the amino terminus of 'passenger' proteins which by themselves cannot enter mitochondria, the resulting fusion proteins are transported into yeast mitochondria. 相似文献
7.
In order to illustrate the ion transport mechanism of chloride channel (ClC) protein, a type of ClC protein, ClC-ec1, from Escherichia coli is embedded into an explicit membranewater system by using software VMD. Then a parallel molecular dynamics (MD) simulation is employed to equilibrate the ClC-ec1 structure for 27.5 ns at temperature 298.15 K. Based on this equilibrated structure, we compute the channel geometric size variation and electrostatic potential distribution along the channel. Meanwhile, Cl- transport process is simulated using oriented random walk method under variable external potential. The simulation result shows that Cl- transport velocity depends on the width of the narrowest channel region. Mutation of negative glutamate E148 can produce positive potential, which is beneficial for Cl- transport, around external Cl- binding region in the channel. The simulated current-voltage curves about Cl- transporting in ClC-ec1 protein agree with Jayaram’s experimental results. 相似文献
8.
WEI Liqin LIU Bo LI Yan 《科学通报(英文版)》2005,50(19):2175-2181
The conventional kinesin and kinesin-related proteins (KRPs) constitute a large family of microtubule-based molecular motors. Members of this family bind to micro-tubules in an ATP-dependent manner. They all have microtubule-activated ATPaes activities, and play essential roles in the transport of various vesicles and organelles in eukaryotic cells[1―3]. The first plant kinesin-like protein was detected in tobacco pollen tube with an antibody against animal kinesin[4]. Recently, a number … 相似文献
9.
Dynein, an ATPase, is the force-generating protein in cilia and flagella. It has long been speculated that cytoplasmic microtubules contain a related enzyme involved in cell division or in intracellular organelle transport. A 'cytoplasmic dynein' has been described in sea urchin eggs, but because the egg stockpiles precursors for both cytoplasmic and ciliary microtubules, the role of this enzyme in the cell has remained unresolved. We recently found that the microtubule-associated protein (MAP) 1C (ref. 6) from brain is a microtubule-activated ATPase that produces force in the direction corresponding to retrograde organelle transport in the cell. MAP 1C has several similar properties to ciliary and flagellar dynein. Here we show directly, using scanning transmission electron microscopy, that MAP 1C is structurally equivalent to the ciliary and flagellar enzyme and is the long-sought cytoplasmic analogue of this enzyme. 相似文献
10.
Segregation of MHC class II molecules from MHC class I molecules in the Golgi complex for transport to lysosomal compartments. 总被引:33,自引:0,他引:33
Traffic of MHC molecules dictates the source of peptides that are presented to T cells. The intracellular distribution of MHC class I and class II molecules reflects the dichotomy in presentation of antigen from endogenous and exogenous origin, respectively. In human B lymphoblastoid cells, class I molecules are present in compartments constituting the biosynthetic pathway, whereas class II molecules enter structures related to lysosomes during their biosynthesis. 相似文献
11.
以Eu2O3、2,2′-联吡啶-3,3′-二羧酸、H2O为原料,采用水热法合成了一种新的具有二维结构的吡啶羧酸类配位聚合物[Eu2(C12H6N2O4)3(H2O)2·5H2O]n,在显微镜下观察该配合物晶体呈无色透明片状。实验采用单一变量法探讨了影响反应的四个因素,得出最佳合成条件为:160℃下恒温72h,降温速度2℃/h,pH=3.4,溶剂为10mL去离子水。X-射线单晶衍射、TGA以及荧光性能测试结果表明:该晶体属于单斜晶系,P2/c空间群;热稳定性较好,骨架在390℃以下可以稳定存在;具有良好的荧光特性。 相似文献
12.
Progression of proteins through the secretory pathway of eukaryotic cells involves a continuous rearrangement of macromolecular structures made up of proteins and phospholipids. The protein SEC14p is essential for transport of proteins from the yeast Golgi complex. Independent characterization of the SEC14 gene and the PIT1 gene, which encodes a phosphatidylinositol/phosphatidylcholine transfer protein in yeast, indicated that these two genes are identical. Phospholipid transfer proteins are a class of cytosolic proteins that are ubiquitous among eukaryotic cells and are distinguished by their ability to catalyse the exchange of phospholipids between membranes in vitro. We show here that the SEC14 and PIT1 genes are indeed identical and that the growth phenotype of a sec14-1ts mutant extends to the inability of its transfer protein to effect phospholipid transfer in vitro. These results therefore establish for the first time an in vivo function for a phospholipid transfer protein, namely a role in the compartment-specific stimulation of protein secretion. 相似文献
13.
The key regulatory enzyme in gluconeogenesis, fructose 1,6-bisphosphatase (FBPase) is subject to glucose-stimulated proteolytic degradation in Saccharomyces cerevisiae. This process involves the regulated transfer of FBPase directly from the cytosol into the vacuole or a vacuole-related organelle. Glucose may regulate the production of an FBPase receptor or import factor that is transported to the vacuole through the secretory pathway. 相似文献
14.
Roles of G protein subunits in transmembrane signalling 总被引:81,自引:0,他引:81
A family of proteins called G proteins couples cell surface receptors to a variety of enzymes and ion channels. Since many cells contain several very similar G proteins, an important question is how signals remain specific as they cross the cell membrane. 相似文献
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16.
Chatterton JE Awobuluyi M Premkumar LS Takahashi H Talantova M Shin Y Cui J Tu S Sevarino KA Nakanishi N Tong G Lipton SA Zhang D 《Nature》2002,415(6873):793-798
The N-methyl-D-aspartate subtype of glutamate receptor (NMDAR) serves critical functions in physiological and pathological processes in the central nervous system, including neuronal development, plasticity and neurodegeneration. Conventional heteromeric NMDARs composed of NR1 and NR2A-D subunits require dual agonists, glutamate and glycine, for activation. They are also highly permeable to Ca2+, and exhibit voltage-dependent inhibition by Mg2+. Coexpression of NR3A with NR1 and NR2 subunits modulates NMDAR activity. Here we report the cloning and characterization of the final member of the NMDAR family, NR3B, which shares high sequence homology with NR3A. From in situ and immunocytochemical analyses, NR3B is expressed predominantly in motor neurons, whereas NR3A is more widely distributed. Remarkably, when co-expressed in Xenopus oocytes, NR3A or NR3B co-assembles with NR1 to form excitatory glycine receptors that are unaffected by glutamate or NMDA, and inhibited by D-serine, a co-activator of conventional NMDARs. Moreover, NR1/NR3A or -3B receptors form relatively Ca2+-impermeable cation channels that are resistant to Mg2+, MK-801, memantine and competitive antagonists. In cerebrocortical neurons containing NR3 family members, glycine triggers a burst of firing, and membrane patches manifest glycine-responsive single channels that are suppressible by D-serine. By itself, glycine is normally thought of as an inhibitory neurotransmitter. In contrast, these NR1/NR3A or -3B 'NMDARs' constitute a type of excitatory glycine receptor. 相似文献
17.
Specificity in bacterial protein synthesis: role of initiation factors and ribosomal subunits 总被引:21,自引:0,他引:21
H F Lodish 《Nature》1970,226(5247):705-707
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19.
Axonal transport of neurotubule protein 总被引:4,自引:0,他引:4
20.
Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein 总被引:43,自引:0,他引:43
At least three different glucose transport systems exist in mammalian cells. These are: (1) the constitutively active, facilitative carrier characteristic of human erythrocytes, Hep G2 (ref. 2) cells and rat brain; (2) the Na-dependent active transporter of kidney and small intestine; and (3) the facilitative carrier of rat liver (B. Thorens and H. F. Lodish, personal communication). A fourth possible glucose transport system is the insulin-dependent carrier that may be specific to muscle and adipose tissue. This transporter resides primarily in an intracellular compartment in resting cells from where it translocates to the cell surface upon cellular insulin exposure. This raises the question of whether hormonal regulation of glucose transport is conferred by virtue of a tissue-specific signalling mechanism or a tissue-specific glucose transporter. Here we present data supporting the latter concept based upon a monoclonal antibody against the fat cell glucose transporter that identifies a unique, insulin-regulatable glucose transport protein in muscle and adipose tissue. 相似文献