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1.
pANGPD是用构巢曲霉GPD基因片段为探针,从黑曲霉基因组文库中筛选到的一个三磷酸甘油醛脱氢酶基因阳性克隆。作者对该阳性克隆进行了亚克隆和序列测定,结果表明,基因片面总长2441个核苷酸,其中5’-端非编码区981n.t,编码区(从起始密码子ATG到终止密友子TAG0长1446n.t.3‘-端非编码区长24n.t,基因启动子区含有盒和CT-盒序列,但无明显的CAAT盒和TATA盒序列。 相似文献
2.
野蚕DHPBAN基因启动子的克隆及序列分析 总被引:1,自引:0,他引:1
为研究昆虫滞育激素性信息素合成激活肽基因在进化过程中的变化以及基因表达调控的异同,用PCR方法克隆了野蚕的DHPBAN基因的启动子并测序,这是第二个被克隆的昆虫DHPBAN基因启动子.与家蚕相比,在核苷酸水平上同源性达94%.野蚕DHPBAN基因启动子的TATA框的保守序列为TATATAAA,位于-47—-40处;CAAT框是GGCCCATCT,位于-83—-75处;野蚕与家蚕一样具有TAAT保守序列,这段序列是一类调控蛋白的结合核心位点,家蚕的位于-185—-176,野蚕的位于-176—-167.在-64—-56部位,核苷酸序列表现出较大的不同. 相似文献
3.
在scu-PA32K的cDNA分子基础上经定点突变,在N端紧接Leu^1以前引入编码GHRP四肽的寡核苷酸序列(GGTCATAGGCCT),构建了GHRP-scu-PA-32K的突变体cDNA。将它克隆到表达载体pCM-β-dhfr共转染CHO/DHFR^-细胞。筛选到的稳定表达株在无 清培养基的表达量为580IR/(10^6细胞.24h)。经锌离子螯合亲和柱纯化的产物,SDS-PAGE显示为一蛋 相似文献
4.
袁汉英 《复旦学报(自然科学版)》1998,37(4):439-444
采用酵母杂合启动子PADHZ-CUP1或PADHZ-GAPDH及终止子TADH1,构建了一系列酵母表达载体。在这些表达载体中插入乙肝有面抗原S-preS1融合基因SA-28后将含乙肝病毒表面抗原的表达单元克隆至高稳定质粒PHC11的BamHⅠ位点。 相似文献
5.
耐热碱性磷酸酯酶基因的亚克隆及其在大肠杆菌中的表达 总被引:1,自引:0,他引:1
根据耐热碱磷酸酯酶基因的酶切图谱和DNA序列,分别在基因的起始密友子和终止密码子设计2个突变引物。引物的5‘端分别带有NdeⅠ和BamHⅠ酶切全点。通过PCR从质粒pTAP118B中扩增获得了FD-TAP基因的全序列。经过酶切将基因亚克隆于高达载体pJLA503。 相似文献
6.
ELECTRONICANDMAGNETICPROPERTIESOFLa0.7-xPrxSr0.3MnO3*GuoZaibingZhangNingDingWeipingZhangJianrongDuYouwei(NationalLaboratoryo... 相似文献
7.
大肠杆菌终止密码子前后序列碱基的统计分析 总被引:5,自引:3,他引:2
统计了大肠杆菌985个基因终止密码子前31个碱基和终止密码子后33个碱基在各个位点的碱基概率分布.发现终止密码子前3个碱基和终止密码子后3个碱基的概率分布与其它位点的概率分布有很大的差别.以TAA结尾的基因序列和以TAG或TGA结尾的基因序列的碱基分布在终止密码子附近也有明显不同.我们又统计了高表达基因和低表达基因终止密码子前后序列的碱基分布,发现在紧邻终止密码子前后3个碱基范围内,某些碱基分布有明显的差别.还发现碱基G和碱基T的3周期分布贯穿整个编码区,碱基A的3周期分布在编码终止区非常明显. 相似文献
8.
扩展青霉(Penicilliumexpansum)PF868脂肪酶的纯化 … 总被引:1,自引:0,他引:1
扩展青霉(P.expansum)PF868所脂肪酶通过硫酸铵沉淀纤维素柱层析以及Sephacryl200柱层析等步骤被纯化了74倍。最终比活力为69.7u/mg。纯化后的脂肪酶经过SDS-PAGE纯和微内径高效液相色谱纯。分子量经SDS-PAGE确定为28.44KD。脂肪酶N-端氨基酸序列测定的结果是:ATADAAAAFPD-这与其他一些真菌产的脂肪酶的序列具有国闫的同源性。 相似文献
9.
用PCR方法获得大肠杆菌二硫键异构酶DsbA的编码基因dsbA和大肠杆菌脯氨酸异构酶PPIaseA的编码基因rot,并将DsbA和rot以双顺反子形式克隆至含有Ptac启动子的表达载体pKK233-2中。在IPTG的诱导下,DsbA和PPIaseA获得了表达。SDS-PAGE和薄层扫描分析表明:DsbA、PPIaseA的表达水平分别为占菌体裂解上清液总蛋白质的4.32%和4.06%。 相似文献
10.
本文将聚ADP核糖聚合酶基因1.4kb部分序列反向插入真核表达载体pMAMneo和pSMG中,同时12位密码子突变的活化ras癌基因也一同克隆到上述载体中,从而获得具有不同真核基因筛选标记的双基因真核表达重组pMAMneo-Cl.4-T24,pMAMneo-Cl.4-arT24,及pSMG-Cl.4-T24,上述质粒的构建成功为研究聚ADP核糖基化作用与细胞恶变的关系提供了一种新的分子模型。 相似文献
11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness. 相似文献
12.
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly. 相似文献
13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。 相似文献
14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death. 相似文献
15.
Miao Maosheng 《华南师范大学学报(自然科学版)》2014,(6)
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula- 相似文献
16.
Yang Weitao 《华南师范大学学报(自然科学版)》2014,46(6):137-138
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re- 相似文献
17.
Bernard Kirtman 《华南师范大学学报(自然科学版)》2014,(6)
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that 相似文献
18.
《上海师范大学学报(自然科学版)》2014,(5):550
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with 相似文献
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20.
《吉林大学学报(理学版)》2014,(5):1105
<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has 相似文献