黑曲霉（Aspergillus niger）三磷酸甘油醛脱氢酶基因（GPD …

ｐＡＮＧＰＤ是用构巢曲霉ＧＰＤ基因片段为探针,从黑曲霉基因组文库中筛选到的一个三磷酸甘油醛脱氢酶基因阳性克隆。作者对该阳性克隆进行了亚克隆和序列测定,结果表明,基因片面总长２４４１个核苷酸,其中５’－端非编码区９８１ｎ．ｔ,编码区（从起始密码子ＡＴＧ到终止密友子ＴＡＧ０长１４４６ｎ．ｔ．３‘－端非编码区长２４ｎ．ｔ,基因启动子区含有盒和ＣＴ－盒序列,但无明显的ＣＡＡＴ盒和ＴＡＴＡ盒序列。     

野蚕DHPBAN基因启动子的克隆及序列分析

为研究昆虫滞育激素性信息素合成激活肽基因在进化过程中的变化以及基因表达调控的异同,用ＰＣＲ方法克隆了野蚕的ＤＨＰＢＡＮ基因的启动子并测序,这是第二个被克隆的昆虫ＤＨＰＢＡＮ基因启动子．与家蚕相比,在核苷酸水平上同源性达９４％．野蚕ＤＨＰＢＡＮ基因启动子的ＴＡＴＡ框的保守序列为ＴＡＴＡＴＡＡＡ,位于－４７—－４０处;ＣＡＡＴ框是ＧＧＣＣＣＡＴＣＴ,位于－８３—－７５处;野蚕与家蚕一样具有ＴＡＡＴ保守序列,这段序列是一类调控蛋白的结合核心位点,家蚕的位于－１８５—－１７６,野蚕的位于－１７６—－１６７．在－６４—－５６部位,核苷酸序列表现出较大的不同．     

GHRP—scu—PA—32K突变体的构建,表达及纯化产物的部分性 …

在ｓｃｕ－ＰＡ３２Ｋ的ｃＤＮＡ分子基础上经定点突变,在Ｎ端紧接Ｌｅｕ＾１以前引入编码ＧＨＲＰ四肽的寡核苷酸序列（ＧＧＴＣＡＴＡＧＧＣＣＴ）,构建了ＧＨＲＰ－ｓｃｕ－ＰＡ－３２Ｋ的突变体ｃＤＮＡ。将它克隆到表达载体ｐＣＭ－β－ｄｈｆｒ共转染ＣＨＯ／ＤＨＦＲ＾－细胞。筛选到的稳定表达株在无 清培养基的表达量为５８０ＩＲ／（１０＾６细胞．２４ｈ）。经锌离子螯合亲和柱纯化的产物,ＳＤＳ－ＰＡＧＥ显示为一蛋     

在杂合启动子控制下乙肝病毒表面抗原SA—28融合基因在酵母中的表达？…

采用酵母杂合启动子ＰＡＤＨＺ－ＣＵＰ１或ＰＡＤＨＺ－ＧＡＰＤＨ及终止子ＴＡＤＨ１,构建了一系列酵母表达载体。在这些表达载体中插入乙肝有面抗原Ｓ－ｐｒｅＳ１融合基因ＳＡ－２８后将含乙肝病毒表面抗原的表达单元克隆至高稳定质粒ＰＨＣ１１的ＢａｍＨⅠ位点。     

耐热碱性磷酸酯酶基因的亚克隆及其在大肠杆菌中的表达

根据耐热碱磷酸酯酶基因的酶切图谱和ＤＮＡ序列,分别在基因的起始密友子和终止密码子设计２个突变引物。引物的５‘端分别带有ＮｄｅⅠ和ＢａｍＨⅠ酶切全点。通过ＰＣＲ从质粒ｐＴＡＰ１１８Ｂ中扩增获得了ＦＤ－ＴＡＰ基因的全序列。经过酶切将基因亚克隆于高达载体ｐＪＬＡ５０３。     

ELECTRONIC AND MAGNETIC PROPERTIES OF La_(0.7-x_Pr_xSr_(0.3)MnO_3

ＥＬＥＣＴＲＯＮＩＣＡＮＤＭＡＧＮＥＴＩＣＰＲＯＰＥＲＴＩＥＳＯＦＬａ０．７－ｘＰｒｘＳｒ０．３ＭｎＯ３＊ＧｕｏＺａｉｂｉｎｇＺｈａｎｇＮｉｎｇＤｉｎｇＷｅｉｐｉｎｇＺｈａｎｇＪｉａｎｒｏｎｇＤｕＹｏｕｗｅｉ（ＮａｔｉｏｎａｌＬａｂｏｒａｔｏｒｙｏ...     

扩展青霉（Penicilliumexpansum）PF868脂肪酶的纯化 …

扩展青霉（Ｐ．ｅｘｐａｎｓｕｍ）ＰＦ８６８所脂肪酶通过硫酸铵沉淀纤维素柱层析以及Ｓｅｐｈａｃｒｙｌ２００柱层析等步骤被纯化了７４倍。最终比活力为６９．７ｕ／ｍｇ。纯化后的脂肪酶经过ＳＤＳ－ＰＡＧＥ纯和微内径高效液相色谱纯。分子量经ＳＤＳ－ＰＡＧＥ确定为２８．４４ＫＤ。脂肪酶Ｎ－端氨基酸序列测定的结果是：ＡＴＡＤＡＡＡＡＦＰＤ－这与其他一些真菌产的脂肪酶的序列具有国闫的同源性。     

大肠杆菌终止密码子前后序列碱基的统计分析

统计了大肠杆菌９８５个基因终止密码子前３１个碱基和终止密码子后３３个碱基在各个位点的碱基概率分布．发现终止密码子前３个碱基和终止密码子后３个碱基的概率分布与其它位点的概率分布有很大的差别．以ＴＡＡ结尾的基因序列和以ＴＡＧ或ＴＧＡ结尾的基因序列的碱基分布在终止密码子附近也有明显不同．我们又统计了高表达基因和低表达基因终止密码子前后序列的碱基分布,发现在紧邻终止密码子前后３个碱基范围内,某些碱基分布有明显的差别．还发现碱基Ｇ和碱基Ｔ的３周期分布贯穿整个编码区,碱基Ａ的３周期分布在编码终止区非常明显．     

大肠杆菌二硫键异构酶DsbA和脯氨酸异构酶PPIaseA双顺反子表达载体的构建及其在大肠杆菌中的表达

用ＰＣＲ方法获得大肠杆菌二硫键异构酶ＤｓｂＡ的编码基因ｄｓｂＡ和大肠杆菌脯氨酸异构酶ＰＰＩａｓｅＡ的编码基因ｒｏｔ,并将ＤｓｂＡ和ｒｏｔ以双顺反子形式克隆至含有Ｐｔａｃ启动子的表达载体ｐＫＫ２３３－２中。在ＩＰＴＧ的诱导下,ＤｓｂＡ和ＰＰＩａｓｅＡ获得了表达。ＳＤＳ－ＰＡＧＥ和薄层扫描分析表明：ＤｓｂＡ、ＰＰＩａｓｅＡ的表达水平分别为占菌体裂解上清液总蛋白质的４．３２％和４．０６％。     

研究聚ADP核糖基化作用与细胞恶变关系的双基因真核表达重…

本文将聚ＡＤＰ核糖聚合酶基因１．４ｋｂ部分序列反向插入真核表达载体ｐＭＡＭｎｅｏ和ｐＳＭＧ中，同时１２位密码子突变的活化ｒａｓ癌基因也一同克隆到上述载体中，从而获得具有不同真核基因筛选标记的双基因真核表达重组ｐＭＡＭｎｅｏ－Ｃｌ．４－Ｔ２４，ｐＭＡＭｎｅｏ－Ｃｌ．４－ａｒＴ２４，及ｐＳＭＧ－Ｃｌ．４－Ｔ２４，上述质粒的构建成功为研究聚ＡＤＰ核糖基化作用与细胞恶变的关系提供了一种新的分子模型。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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