利用分子标记分析22种水稻10个抗稻瘟病基因的基因型

为了明确已克隆的部分稻瘟病抗性基因在上海市主栽水稻品种以及新培育的水稻品系中的分布,选取22份水稻为材料,利用分子标记检测技术,对10个已被克隆的抗稻瘟病基因进行基因型鉴定及分析.结果表明,22种水稻均含Pi36、Pi37、Pi-d2、Pib抗性基因,而Pi-kh、Pi9、Pi2、Pi-ta、Pikm抗性基因在22种水稻中分布各有不同,所有被测水稻中均不携带Pi25抗性基因.     

上海市11种常规粳稻抗稻瘟病基因分子标记检测

利用分子标记检测技术,对9种参加2020年上海市水稻区域试验的品种和2种本课题组新培育的新品系的共10个抗稻瘟病基因位点进行了检测.结果显示,Pi37,Pi41,Pi-d23个基因在11种水稻中出现的频率达100％,Pi2,Pi5,Pi9,Pi36,Pikm和Pib抗性基因在11种水稻中出现的频率分别为18.18％,9...     

稻瘟病抗性基因Pita2的克隆及其介导的防御相关基因表达分析

为了克隆位于水稻12号染色体着丝粒区域具有广谱抗性的稻瘟病抗性基因Pita2,研究构建并筛选了该基因供体品种PiNo.4的Fosmid文库,构建Pita2候选区域的物理图谱并互补验证了5个候选基因,克隆得到了稻瘟病抗性基因Pita2,确认了该基因就是Ptr基因.一组基因型接近、对Pita2致病性相反的稻瘟病菌菌株对水稻...     

云南地方稻种抗稻瘟病基因Pita和Pib的研究进展

稻瘟病是水稻主要病害之一,选育抗稻瘟病品种是防治稻瘟病非常有效的方法.总结了稻瘟病抗性基因特别是主效抗病基因Pita和Pib的定位、克隆及其功能等方面的研究进展,并对利用云南丰富的稻种资源挖掘抗性基因做了展望.     

稻瘟病抗性基因 Pi63 启动子4 个构建体的表达及抗性分析

利用农杆菌介导法获得带有不同长度启动子区域的稻瘟病抗性基因Pi63全长基因的转基因水稻,并采用实时荧光定量PCR方法鉴定了T1代阳性植株的基因拷贝数,进一步测定了单拷贝转基因植株在接种稻瘟病菌前后Pi63基因的表达量变化.结果表明:接种后4个分别带有不同长度启动子的Pi63转基因植物T1代中Pi63基因的表达量均升高,其抗病相关的顺式作用元件处于P1与P2中间的区域,P0到P1的区域存在负调控元件.此结果可为进一步揭示水稻稻瘟病抗性基因Pi63的分子机理奠定基础.     

转多个抗真菌蛋白基因水稻植株的获得及其抗稻瘟病菌的初步研究

用基因枪法将水稻碱性几丁质酶（ＲＣ２４）基因、苜蓿葡聚糖酶（βＧｌｕ）基因、大麦核糖体失活蛋白（ＢＲＩＰ）基因和潮霉素（ｈｐｔ）基因同时导入籼稻品种（七丝软占）中,获得了７个潮霉素抗性再生系,Ｓｏｕｔｈｅｒｎｂｌｏｔ证明２～３个抗真菌蛋白基因已整合到水稻基因组中．初步抗性鉴定表明Ｒ０代转基因水稻植株对稻瘟病菌的抗性有所提高     

利用分子标记辅助选育优质香软水稻新品系“上师大22号”及试种

为培育适合上海市及其周边地区种植的优质香软水稻，以优质水稻“银香38”为母本，以抗稻瘟病水稻品系“13-2”为父本，进行杂交获得了F1种子；再以抗倒性较强的“武运29185”香稻为母本，与F1植株进行复交，然后多代自交，期间利用香味基因和抗稻瘟病基因分子标记辅助筛选，成功培育出优质香软水稻“上师大22号”.试种结果显示：“上师大22号”水稻全生育期比目前上海市主栽常规水稻“秀水134”短12 d，接近“银香38”水稻；“上师大22号”亩产量分别比“秀水134”和“银香38”增加2.84%和3.96%；“上师大22号”稻瘟病抗性及抗倒性都比“银香38”明显提高.“上师大22号”水稻新品系的成功培育，为上海市进一步推广优质稻品种奠定了基础.     

分子标记辅助选育含有抗稻瘟病基因和软米基因两系不育系水稻新品系

以"浦软粳S"为转育亲本,利用分子标记辅助常规育种技术成功培育出含有Pi9,Pita,Pib和Pigm稻瘟病抗性基因及软米基因(Wx~(mq)),同时表现柱头外露率高的两系不育系水稻新品系"2179S"."2179S"不育系茎秆粗壮,矮杆大穗,株高为63.8 cm,柱头外露率平均为60%.研究结果为今后培育具有稻瘟病抗性的优质两系杂交水稻新组合提供不育系亲本.     

谈水稻育种高世代材料抗稻瘟性的鉴定

稻瘟病是世界上各稻区分布最广泛的严重病害之一.随着人们对优质米的重视和对日本品种的大量引进种植,稻瘟病在我省局部地区又有回升趋势,选用和培育抗病品种是综合治理该病的经济有效措施.现将稻瘟病抗性鉴定方法简报如下,旨在为水稻抗病育种提供科学依据,加速抗病育种进程.1 材料与方法     

转多个抗真菌蛋白基因水稻植株的获得及其抗稻瘟病菌的？…

用基因枪法将水稻碱性几丁质酶（ＲＣ２４）基因、芷蓿葡聚糖酶（β－Ｇｌｕ）基因、大麦核糖体失活蛋白（Ｂ－ＲＩＰ）基因和潮霉素基因同时导入籼稻品种（七丝软占）中,获得了个潮霉素抗性再生系,Ｓｏｕｔｈｅｒｎ ｂｌｏｔ证明２－３个抗真菌蛋白基因已整合到水稻基因组中,初步抗性鉴定表明ＲＯ代转基因水稻植株对稻瘟病菌的抗生有所提高。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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