Effect of carbon particles on the recovery of bone marrow stem cells after irradiation in LPS-resistant C3H/HeJ mice

Effect of RES-blockade on bone marrow cells was studied serially after irradiation in LPS-resistant mice. Injection of carbon particles reduced damage and accelerated recovery of marrow hemopoietic stem cells, indicating that LPS-resistant mice can react normally to RES-blockade.     

Effect of carbon particles on the recovery of bone marrow stem cells after irradiation in LPS-resistant C3H/HeJ mice

Summary Effect of RES-blockade on bone marrow cells was studied serially after irradiation in LPS-resistant mice. Injection of carbon particles reduced damage and accelerated recovery of marrow hemopoietic stem cells, indicating that LPS-resistant mice can react normally to RES-blockade.This work was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan.     

Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide ofPorphyromonas gingivalis

Porphyromonas gingivalis 381 lipopolysaccharide (LPS) definitely exhibited mitogenic activity in purified B-cells, separated from spleens of LPS-responsive C3H/HeN mice and LPS-non-responsive C3H/HeJ mice by using a magnetic cell sorting system. The mitogenic activity induced byP. gingivalis LPS was incompletely inhibited by polymyxin B.P. gingivalis LPS also induced a higher production of interleukin-6 (IL-6) in splenic B-cells of C3H/HeN mice as compared withEscherichia coli LPS. Furthermore,P. gingivalis LPS, but notE. coli LPS, induced definite IL-6 production in C3H/HeJ mice.P. gingivalis LPS increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B-cells of both C3H/HeN and C3H/HeJ mice. Additionally, radioiodinatedP. gingivalis LPS, similarly toE. coli LPS, bound to a 73-kDa protein on C3H/HeJ as well as C3H/HeN B-cells. ThusP. gingivalis LPS may activate B-cells of C3H/HeJ as well as C3H/HeN mice via the LPS-specific binding protein on the cells.     

Increased melatonin levels after hemorrhagic shock in male and female C3H/HeN mice

Although hemorrhagic shock leads to significant alterations of several hormones, e.g. ACTH, corticosterone and -endorphin, it is not known whether plasma melatonin levels are affected under this condition and if so, whether the effects are comparable in males and females. Using a radioimmunoassay, it was found that plasma melatonin levels were significantly increased in male and proestrus female C3H/HeN mice immediately after hemorrhagic shock. However, in male mice, by two hours after hemorrhage and resuscitation, plasma melatonin returned to levels comparable to those seen in control and sham-operated animals. Proestrus female mice, on the other hand, showed significantly increased plasma melatonin levels at two hours after surgery when compared to unoperated control animals. Although the significance and biological role of the transient increased plasma melatonin levels after hemorrhagic shock remain to be determined, it appears that the pineal gland and/or an extrapineal source of melatonin, of both male and proestrus female mice responds to severe hypotension by increased release of melatonin.     

Renal regeneration in chloroform-poisoned male mice of strain C3H/He treated with di-sodium versenate

Résumé L'intoxication au chloroforme des souris mâles de la souche C3H/He entraîne la mort due à la nécrose et à la calcification tabulaire rénale. Le traitment par le versenate de sodium prévient la calcification ce qui rend possible la régénération des tubes contournés renaux.     

Influence of chronic treatment with 2-bromo-α-ergocryptine (CB-154) on the reproductive and lactational performance of the C3H/HeJ female mouse

Zusammenfassung C3H/HeJ-weibliche Mäuse, die vor der Paarung dauernd mit CB-154, einem stark wirkenden Mittel zur Unterdrückung der Prolactinsekretion, behandelt wurden, wiesen keine bedeutsame Schädigung ihrer Fortpflanzungsfähigkeit auf. CB-154 Dauerbehandlung von weiblichen Mäusen sowohl während der Paarung als auch während der Laktation führte zu einer bedeutsamen Herabsetzung der Fruchtbarkeit und Milchabsonderung.

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Supported by NIH research grant No. CA-13777 and American Cancer Society research grant No. ET-59.

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NIH Research Career Development Awardee No. CA-35027

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CB-154 was provided through the courtesy of Dr.Richard Elton, Sandoz Pharmaceuticals, East Hanover, N. J., USA.

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Thanks are given toMs. Carol Gribler for her assistance in this study.     

Induction of lactation in precancerous hyperplastic alveolar nodules in the mammary gland of C3H/He Crgl mice

Résumé La sécrétion lactée se produit dans les nodules alvéolaires hyperplastiques de la glande mammaire des souris femelles intactes de la souche C3H/He Crgi, à la suite de l'administration du cortisol et de l'stradiol, mais non pas après l'emploi de l'strogène avec le progestérone. Lorsque l'hypophyse, l'ovaire et la glande surrénale ont été enlevés, les nodules montrent une sécrétion lactée sous l'effet du cortisol et de la mammotropine ou de la somatotropine et indiquent une sensibilité différentielle aux combinaisons hormonales lactogènes.     

Organ weights of TE mice bearing ehrlich ascites carcinoma growing in the solid form and of C3H mice bearing spontaneous mammary carcinoma

Zusammenfassung Das Milzgewicht karzinomtragender Mäuse ist dreimal so hoch wie das Milzgewicht gesunder Kontrollen.Die Leber karzinomtragender Mäuse ist ungefähr anderthalbmal so schwer wie die Leber gesunder Kontrollen.Das Nierengewicht von C3H-Mäusen mit Mammakarzinom beträgt nur etwas mehr als die Hälfte des Nierengewichtes gesunder Kontrollen.Intraperitoneale Injektion von 0,25 ml Vollblut oder 0,2 ml Plasma von TE-Mäusen mit Ehrlich-Ascites-Karzinom in der solid wachsenden Form erhöht das Milzgewicht gesunder Kontrollen auf das Doppelte.     

Histochemische Untersuchungen während der Wallerschen Degeneration bei C3H/An und C57bl-Mäusen

Summary The reduction of the myelin sheath during the Wallerian degeneration is not delayed in C3H/An mice with reduced activity of-d-glucuronidase. After section of the sciatic nerve, an increase of glucuronidase activity in Schwann's cells and macrophages can be shown here too.     

Preliminary virological and immunological studies of the salivary gland tumor of C3H/He mouse

Riassunto Dai dati riferiti si mettono in evidenza due punti: il primo dimostra nel tumore in esame l'assenza di particelle virali e di effetto citopatico specifico per il virus polioma. Inoltre non si mettono in evidenza anticorpi fissanti il complemento o emoagglutinoinbenti per il virus polioma negli animali portatori di tumori. II secondo punto dimostra che durante il suo sviluppo il tumore della ghiandola salivare é incapace di indurre una risposta immunologica apprezzabile.     

Oncogenicity of a cell line derived from adenocarcinoma of the salivary gland of C3H/He mouse

Riassunto Cellule portatrici di virus derivate da adenocarcinoma della ghiandola salivare coltivate in vitro sono oncogeniche per l'ospite isogenico per circa sei mesi di coltura. Dopo tale periodo perdono l'oncogenicità e non è più possibile ritrovare particelle virali. Sulla base di questi risultati si conclude che il virus è l'agente causale dell'adenocarcinoma della ghiandola salivare del topo C3H/He.     

On the in vitro behaviour of mouse submaxillary gland cells. II. Metabolic differences between male and female C3H mice

Summary Submaxillary gland cells from female C3H mice were isolated, cultivated in vitro and their metabolic properties compared with those of male derived cells. From the results it can be concluded that these cells retain their metabolic differences when grown in vitro.This work was supported by grant No. CT 73.00642.04 from the Consiglio Nazionale delle Ricerche (C.N.R.), Roma, Italy.