Demonstration von Elementarfibrillen in menschlichen Metaphasechromosomen in situ

Summary Tightly packed elementary fibrils with a diameter of 20 Å are demonstrated in human metaphase chromosomes in situ.

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Demonstration von Elementarfibrillen in menschlichen Metaphasechromosomen in situ

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Für die Mithilfe bei der Präparation danke ich FrauM. Brun del Re; für die Aufnahmen mit dem Philips EM 300 bin ich HerrnJ. di Lullo vom Veternärmedizinischen Institut der Universität Bern zu Dank verpflichtet.     

Die Isolierung von Ribonukleinsäuren aus menschlichen Knochenmarkzellen

Summary By use of bentonite and sodium dodecylsulfate, which bind or inhibit ribonucleases, pure RNA preparations were obtained from human bone marrow. Phenol extraction at 4 °C yielded cytoplasmic RNA, which could be separated into 3 main peaks by sucrose density gradient centrifugation. Sedimentation coefficients of these peaks were about 30S, 19S, and 5S, respectively. Re-extraction of the phenolic interphase at 65° gave a polydisperse ribonucleic acid, sedimenting between 2S and 19S. This RNA is characterized by template activity and is supposed to be biologically active m-RNA.     

Demonstration of post-hypophysectomy changes in the neurosecretory system of a fish with in situ staining technique

Zusammenfassung Bei einer Fischart,Rita rita (Ham), wird nachgewiesen, dass sich nach Entfernung der Hypophyse im Gebiet proximal davon Substanzen nachweisen lassen, die von einer vermehrten Sekretion aus dem Gebiet des Hypothalamus stammen müssen.     

Über die Wirkung von 7,12-Dimethylbenz(a)-anthrazen auf die Synthese der Lactatdehydrogenase-Isoenzyme in menschlichen Fibroblasten

Summary The effect of 7,12-Dimethylbenz(a)anthracene (DMBA) on the activity of lactate dehydrogenase (LDH)-isoenzymes was studied in human fibroblast cultures. 3 isoenzyme components were separated by discelectrophoresis. A clear reduction in the enzymeactivity of the first and third component was observed, while the other fraction was little or not affected. The incorporation of radioactive amino acids in proteins of the LDH-isoenzyme bands shows the same trend.     

Zur Frage der Neubildung von Primordialfollikeln aus dem Keimepithel während der Geschlechtsreife im menschlichen Ovar

Summary Findings in the human ovary confirmed the fact already pointed out in animal ovaries that under the influence of oestrogens the germinal epithelium is capable of forming new oocytes. Whether the germinal epithelium of the mature woman can form new oocytes under normal conditions cannot be determined as yet, but the present findings allow the conclusion that neo-oogenesis during the reproductive period of women seems possible. Further investigations, now in progress, are required to determine this possibility.     

Zum Problem der menschlichen Stammesgeschichte

Ohne Zusammenfassung     

Der Oxytocingehalt im menschlichen pathologischen Liquor

Summary The quantity of oxytocin in the cerebrospinal fluid is decreased by brain atrophy and infantile brain damage, not injured by multiple sclerosis but increased by meningitis, sciatica and drug controlled convulsions. Immediately after a seizure, the oxytocin level is very strong raised.

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Diese Arbeit wurde mit Mitteln des Ministeriums für Wissenschaft und Technik der DDR durchgeführt.     

Zur Lokalisation der Östrogenproduktion in der menschlichen Plazenta

Summary The syncytial sprouts of the human placenta were found to give a distinctly stronger reaction for glucose-6-phosphat-dehydrogenase, DPNH-diaphorase and steroid-3--ol-dehydrogenase than other regions of the trophoblastic syncytium. Besides they show a more intensive basophilic reaction and higher contents of fat than other parts of the syncytium. Therefore the syncytial sprouts can be regarded as the areas of the trophoblastic syncytium that are specialized for estrogen production.

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Herrn Prof. Dr.K. Goerttler zu seinem 70. Geburtstag gewidmet. — Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.     

Faserspezifischer Aufbau und Digitalisgehalt der menschlichen Skelettmuskulatur

Summary In 2 patients digitalized with digoxin or betamethyldigoxin, postmortal glycoside concentrations were determined in 7 different skeletal muscle specimens by radioimmunoassay. In the same specimens, planimetric measurements of histochemical fibre types I and II were carried out. There were higher glycoside concentrations in predominantly type I fibre muscle biopsies.

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Nach einem Vortrag auf der Arbeitstagung der Deutschen Gesellschaft für Neurologie und der Deutschen Gesellschaft «Bekämpfung der Muskelkrankheiten» in Würzburg, Mai 1976.

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Die Untersuchungen wurden mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt. Wir danken Frl. A. Berens für die technische Assistenz.     

Interzelluläre Verbindungen beim Plattenepithelkarzinom der menschlichen Haut in der Gewebekultur

Summary A new medium was developed for tissue-culturing of the human squamous cell carcinoma of the skin. The morphology of the outgrowing tumour cells is described, particularly with regard to the projections of cell surface. There are 2 forms to be differentiated: one which is similar to the desmosomes of the normal epithelium of the skin, and one of intercellular connective strands. The different functions of both forms are discussed with regard to the exchange of cell matter.     

Demonstration of particulate accumulations in solid tissue

Summary We describe a histological clearing procedure involving partial tissue hydrolysis by pancreatin and demonstrate its application. Changes in distribution of 5.7-m latex particles in mouse liver and heart muscle 6 months after i.v. latex injection were quantitated. The method is useful for studies of long-term redistribution of particles in vivo and for locating large, infrequent particles which are difficult to find by ordinary tissue-sectioning techniques.Acknowledgments. Research supported by U.S. Department of Energy under contract EY-76-C-02-0016 and U.S. Environmental Protection Agency under contract 79-D-X-05333. The assistance of Mr Willie White is gratefully acknowledged. We are indebted to Mr William Marin for photomicrography.The research described in this report involved animals maintained in animal care facilities fully accredited by the American Association for Accreditation of Laboratory Animal Care.     

Demonstration of two ATPases in human erythrocyte membranes

Zusammenfassung Menschliche Erythrozytenmembranen enthalten eine NEM-sensitive und eine NEM-insensitive ATPase, die wahrscheinlich beide an Membranoberflächen lokalisiert sind.

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These studies were carried out with the generous support of the Deutsche Forschungsgemeinschaft.