HMGB1对心肌成纤维细胞增殖和Ⅰ、Ⅲ胶原蛋白分泌的影响及意义

摘要：目的：通过观察HMGB1（ High mobility group protein-B1）对新生SD（Sprague-Dawley）大鼠心肌成纤维细胞(CFs)增殖和胶原合成的影响,探讨HMGB1在心梗后心脏重构过程中的作用。方法：以培养的新生SD（Sprague-Dawley）大鼠CFs为实验对象,分别给予0(对照组)、0.01、0.1、和1 mg/L 浓度的HMGB1进行干预,6、12、18、24和48小时后进行四氮唑盐(MTT)比色法检测CFs增殖水平,采用荧光实时定量PCR法检测Ⅰ、Ⅲ型胶原蛋白mRNA表达。结果：1.与对照组相比,48小时后,不同浓度的HMGB1组新生SD大鼠CFs增殖水平和Ⅰ、Ⅲ型胶原mRNA的表达水平均较对照组有所增高(p<0.05)。2.HMGB1浓度为0.1mg/L时CFs增殖水平和I、Ⅲ型胶原mRNA表达水平较其他处理组均升高(p<0.05)。结论：HMGB1能促进SD大鼠CFs增殖及Ⅰ、Ⅲ型胶原蛋白mRNA的表达,其作用显示在低水平时可能呈浓度依赖性,并且浓度为0.1mg/L时效应最明显,结果显示HMGB1参与了心梗后的心脏重构过程并发挥了重要作用。     

Cell contact- and shape-dependent regulation of vinculin synthesis in cultured fibroblasts

Recent studies have demonstrated the fundamental role of cell-substrate contacts and changes in cell shape in the regulation of cell growth, motility and differentiation, but the molecular basis for these phenomena is poorly understood. Because of the involvement of cytoskeletal networks in cell morphogenesis and contact formation, it is of interest that the expression of genes encoding several cytoskeletal proteins is markedly affected by changes in cell contacts and configuration. Because most of these phenomena involve changes in the form, extent or topology of cell contacts, we sought to determine whether the expression of components directly involved in the formation of cell-cell or cell-substrate contacts is affected by the respective cellular interactions. A suitable candidate for such analysis is vinculin, a cytoskeletal protein of relative molecular mass (Mr) 130,000 (130K), which is localized in focal contacts and intercellular adherens junctions. The assembly of vinculin into a membrane-bound junctional plaque seems to be one of the earliest cellular responses to contact with exogenous substrates, leading to the subsequent local assembly of the actin-rich microfilament bundles. Here we report on the regulation of vinculin synthesis in response to environmental conditions that affect cell shape and contacts.     

Metabolites of diethylstilboestrol induce sister chromatid exchange in human cultured fibroblasts.

Diethylstilboesterol (DES) is one of the few substances for which a clear association with carcinogenicity has been established in man. Nevertheless, it is still widely used, mainly as a cheap oestrogen to increase the slaughter weight of beef, but in spite of this it is not known if residues in the meat or metabolites excreted by the cattle are hazardous to man. It is also unknown whether there is a threshold dose below which DES is harmless. A threshold might be expected if a hormonal mechanism of carcinogensis rather than metabolic activation to an electrophically reactive species operats. This possibility was supported by the observations that DES, in contrast to most other carcinogens, failed to induce mutations in the Salmonella/microsome test or malignant transformations of eukaryotic cells in culture. It is also disturbing that DES, one of the few known human carcinogens was negative in these two most widely used short-term tests introduced as fast early-earning system for potential carcinogens. We now report that DES is positive in sister chromatid exchange (SCE) induction, a short-term test for which a high correlation with the carcinogenicity of the compounds tested has been observed. Moreover, we show that metabolic activation was involved. Two different pathways leading to metabolites much more active in SCE induction than DES itself ('proximate agents') were established.     

基于自相关理论的GM(1,1)和GM(1,N)联合预测

通过引入自相关分析，将GM（1，1）与GM（1，N）两者的优点有机结合，运用GM（1，1）预测模型所需的数据量，达到GM（1，N）预测模型所具有的预测精度，减少灰色模型的预测误差。     

非等间距GM(1,1)模型的改进及预测分析

论述了非等间距GM(1,1)模型的基本原理及精度评定方法,针对模型缺陷探讨了模型精度的主要影响因素,并就初值选取及模型背景值构造2个方面进行改进,建立了改进的非等间距GM(1,1)模型.运用改进的非等间距GM(1,1)模型对某坝堤沉降进行预测分析,结果表明,改进后的非等间距GM(1,1)模型较改进前具有更高的预测精度,在沉降预测中具有较高的应用价值.     

不同类型水稻在氮素吸收及利用上的差异

在群体水培条件下,测定了95个水稻材料由抽穗期及成熟期植株的含氮率及干物质积累量,并分常规粳稻、常规籼稻、广亲和品种、杂交粳稻、杂交籼稻及两系杂交稻6种类型,研究了供试水稻材料在氮素吸收及利用上的差异,结果表明：①籼稻梗株的含氮率明显主粳稻及广亲和品种,杂交灿稻的含氮率高于常规籼稻,而杂交粳稻的含氮率与常规粳稻无显著差异;②籼稻植株总吸氮量平均比粳稻高154．1％,与常规籼稻及常规粳稻相比,杂交籼稻及杂交粳稻的总吸氮量分别高22．8％和16．4％。③植株总吸氮量在器官中的分配比例以根、叶片的变异幅度较大,而穗及茎鞘的变异幅度则较小;根中氮的分配比例以广亲和品种最高,常规灿稻显著高于杂交灿稻,而常规稻而杂交精稻间无显著差异。④从氮的利用效率看,粳稻及广亲和品种氮干物质生产效率高于籼稻,而氮的粒粒生产效率低于灿稻;氮收获指数以广亲和品种最低,其他类型间差异未达显著水平。     

G M(1，1)模型在安全事故率预测中的应用

应用灰色系统模型GM(1,1)对工厂生产过程中安全事故率进行预测,获得了满意的结果.这对于工厂有效地控制安全事故的发生,减少其造成的经济损失起到了重要的作用.     

大型班轮公司多级伙伴协作优化顺序GM(1,2,x)模型

在建立大型班轮公司供应链或物流系统多级伙伴协作总响应时间网状结构模型的基础上，定义多伙伴协作主响应时间的概念，构建多伙伴协作主响应时间结构模型，进一步地构建多伙伴协作主响应时间效率GM（1，2，x）模型．结合该模型，给出数据的应用分析，根据该模型计算的结果，为基于缩短多级伙伴协作总响应时间的大型班轮公司管理者给出相应的应对策略．     

灰色预测模型GM(1,1)及其在山东省GDP预测中的应用

运用灰色理论研究了山东省国内生产总值(GDP)1996年至2005年的变化规律,建立了山东省国内生产总值预测的灰色模型,并对2006年至2010年山东省国内生产总值作了预测.利用灰色关联度分析,分析了山东省第一、二、三产业对国内生产总值的影响程度.     

GM(1,1)和GM(1,N)联合模型在自来水厂自动加矾系统预测中的应用

通过引入自相关分析,将GM(1,N)模型预测精度高的优点和GM(1,1)所需统计数据数量少的优点,两者有机结合形成一个联合预测模型,运用于自来水厂自动加矾系统,进一步提高灰色模型的预测精度,并证明了该方法在实际应用中的可行性.     

GM(1,N)模型的多重共线性的产生及岭估计改进法

将累加处理表示成矩阵的形式，并再此基础上证明了某特殊情况下，累加处理将产生多重共线性，并且用岭估计法改进了灰色系统模型。     

FX_（2N）-20GM在平行缝焊机中的应用

介绍了三菱运动控制模块FX2N-20GM对步进电机的控制及其在平行缝焊机传动部分的具体应用,指出在平行缝焊机中运用PLC结合20GM特殊模块设计的运动系统可靠性高,编程简单易懂,可在多轴运动控制系统中推广应用。