生物电化学传感器研制及应用的进展

本文介绍了生物电化学传感器的制备及应用的进展.生物电化学传感器包括酶、微生物、组织、免疫和细胞器传感器.     

压电生物传感器研究进展

简要介绍了压电传感器的基本原理，并从气态物质分析，微生物分析，细胞分析，生物过程监测，反应动力学分析，蛋白质分析，核酸分析，酶分析以及生物小分子物质分析等方面介绍了压电传感器在生物分析领域的研究进展。     

谷氨酸生物传感器的研制

采用海藻酸钙凝胶包埋法及海藻酸钙凝胶包埋与戊二醛化学交联相结合的固定化方法，制备了固定化谷氨酸脱羧酶膜，与ＣＯ２气敏电极偶联制成谷氨酸生物传感器。经测试，对谷氨酸的线性响应范围为（１．０×１０（－２）～２．０×１０（－４））ｍｏｌ／Ｌ，斜率为５５．７ｍＶ，检测下限为２．３×１０（－４）ｍｏｌ／Ｌ，稳态响应时间为（９～１５）ｍｉｎ。用包埋与交联相结合制得的传感器，其稳定性优于仅用包埋法制得的传感器。     

有机磷生物传感器的研制

有机磷生物传感器对于环境分析十分必要.利用乙酰胆碱酯酶与有机磷农药之间的特殊的亲和力,以乙酰胆碱酯酶为固定相,采用石英晶体用来做载体的材料,研制有机磷传感器.初步得到酶固定的最佳pH值及其浓度环境为pH=7,乙酰胆碱酯酶浓度为C=0.0133mol.l-1.并对敌百虫溶液进行测定,证明了pH值和浓度条件的合理性.     

生物传感器的研究状况

随着对生物感觉系统的研究及现代电子技术和生物工程的迅猛发展,使得生物传感器应运而生。生物传感器实际上是化学传感器的一个分支,从结构上来讲,生物传感器由两个部分组成,第一部分就是感受器,它是由具有分子识别能力的生物活性物质构成,如酶、抗原、抗体、动植物组织切片或微生物等。第二部分是基础电极或称内敏感器,这是由电化学或光学检测元件组成,如电流或电位测量电极、压电晶体、热敏电阻、场效应晶体管和光纤等。生物传     

DNA生物传感器的研究进展

核酸生物传感器在涉及分子生物学的研究领域具有重要意义。为适应分子生物学及其相关学科的发展需要,其研究正成为当前生物传感技术研究热点。文章对核酸生物传感器的工作原理、分类、研究现状以及发展趋势作了评述。     

用酪氨酸酶生物传感器测水中酚类物质的研究

研究了用麦芽糊精修饰的酪氨酸酶碳糊电极构成电流型生物传感器测水中酚类污染物质的方法 .在外加电压为 - 1 0 0 m V( vs.SCE) ,p H值为 5 .40的磷酸盐缓冲溶液中 ,在浓度为 2 .0×1 0 -7～ 1 .0× 1 0 -5mol/L的范围内电极与苯酚的浓度有良好的线性关系 ,检出下限为 1 .0× 1 0 -7mol/L ,响应时间为 2 min.此电极对其他酚类物质如 :邻苯二酚 ,对氯苯酚 ,邻甲酚等都有良好的响应 .利用此种电极可用来检测工业废水中的酚类物质的含量     

Recombinant soluble TRAIL induces apoptosis of cancer cells

TRAIL is a tumor necrosis factor family member that selectively induces apoptosis of cancer cells but not of normal cells. To develop TRAIL into a potential cancer drug, three different sizes of soluble TRAIL fragments, including sTRAIL(74—281), sTRAIL(95—281) and sTRAIL(101—281), were expressed in E. coli and purified to homogeneity. Apoptosis assays indicated that sTRAIL(95—281) and sTRAIL(101—281), but not sTRAIL(74—281), can potently induce apoptosis of various cancer cell lines in 6 h, suggesting that the N-terminal fragment of aa101 has inhibitory effect on TRAIL-induced apoptosis. Moreover, we found that some cancer cells were resistant to TRAIL and the resistant cells could be converted into sensitive cells by treatment with the protein synthesis inhibitor cycloheximide, suggesting that one or more short-lived proteins are responsible for cells’ resistance to TRAIL.     

The molecular mechanism of different sensitivity of breast cancer cell lines to TRAIL

Although Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis of various cancer cells, some caner cell lines are resistant to TRAIL-induced cell death. To investigate the molecular mechanisms underlying TRAIL-resistance, two human breast cancer cell lines, MCF-7 (resistant to TRAIL) and MDA-MB-231 (sensitive to TRAIL), were used as a model system to analyze the different sensitivities to TRAIL cytotoxicity. PKCδ inhibitor rottlerin, but not MEK and ERK1/2 inhibitor U0126 nor PI3K inhibitor LY294002, was shown to enhance TRAIL-induced apoptosis in MCF-7 cells significantly, suggesting that PKCδ might play an important role in the resistance of MCF-7 cells to TRAIL. In contrast, rottlerin, U0126, and Ly294002 had no effect on MDA-MB-231 apoptosis induced by TRAIL under the same conditions. Further experiment showed that the combination of rottlerin and TRAIL cleaved PARP in the MCF-7 cells synergistically, but not in the MDA-MB-231 cells. The role of PKCδ in TRAIL-resistant MCF-7 cells was confirmed by knocking down the endogenous PKCδ expression using RNAi technology. Furthermore, caspase-3 reconstitution in MCF-7 cells was unable to alter PKCδ expression, suggesting that innate caspase-3 deficient in the cells does not cause PKCδ high expression. These data provide evidence for the first time that PKCδ plays a critical role in breast cancer cell lines to TRAIL cytotoxicity.     

葡萄糖氧化酶在丝素膜中的固定化及其性能和应用于生物传感器(英文)

应用二种中性盐溴化红和氯化钙作为溶解蚕丝丝素的试剂,制成了二种固定化葡萄糖氧化酶丝素膜。经酶比色法和红外分光光度法分析,结果表明这二种丝素膜都是良好的固定化酶的生物材料。葡萄糖氧化酶经这些丝素膜固定以后对热和pH稳定性得到明显改良。这些酶膜性能稳定,具有高的活性得率,能长期存放。以这些固定化葡萄糖氧化酶丝素膜和氧电极为基础,研制的流动注射分析式电流型葡萄糖生物传感器性能较稳定,具有较宽的葡萄糖线性响应范围(0.5～15.0mmol/L),相关系数为0.999,可以反复测定葡萄糖一千次以上。     

Human Soluble TRAIL Protein Inducing Apoptosis in Osteosarcoma Cell

This study is to examine the effect of human recombinant soluble TRAIL （TNF-related apoptosis-inducing ligand） protein inducing apoptosis in MG-63 human osteosarcoma cells. The inhibitive rates of TRAIL to MG-63 cells were detected by MTT assay. The apoptosis induced by TRAIL in MG-63 human osteosarcoma cells was analyzed with FACS and TUNEL and the apoptotic bodies were observed by transmission electron microscope. MTT assay showed that the inhibitive rates of 500, 1 000, 2 000 and 4 000 ng/mL TRAIL for 24 h were 10.1%, 24.3%, 50.6% and 97.7% respectively. Flow cytometric analysis showed that after MG-63 cells were treated with 2 gg/mL TRAIL for 6 h, obvious apoptotic peak would immediately appear before diploid peak. Human soluble TRAIL protein can quickly kill MG-63 osteosarcoma cells selectively, and may have potential value for clinical treatment of osteosarcoma.     

关于我国幼儿体育教育目标的研究

幼儿体育教育目标是形成终身体育思想的前提和依据．通过对幼儿体育教育目标现状、作用的分析和各种幼儿体育教育思想的比较，以及对幼儿体育教育思想理论基础的研究〔２〕，探讨了我国幼儿体育教育目标确立、统一的重要性和可行性     

伤寒沙门菌激活TRAIL信号通路促进巨噬细胞凋亡

为探究伤寒沙门菌对巨噬细胞凋亡及肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis inducing ligand,TRAIL)信号通路的影响,对伤寒沙门菌感染THP-1细胞早期和后期提取细胞RNA进行转录组测序(RNA-seq).通过定量PCR(qPCR)技术...     

对我院女新生身体素质状况的调查及其对策探讨

通过调查发现，大学女新生身体素质水平普遍较低，重智轻体现象仍很严重。本文分析了造成这一状况的原因，并提出了相应的对策．     

对我院女新生身体素质状况的调查及其对策探讨

通过调查发现,大学女新生身体素质水平普遍较低,重智轻体现象仍很严重。本文分析了造成这一状况的原因,并提出了相应的对策。