Clonal origin of haematopoietic colonies in the postnatal mouse liver

The liver of the neonatal mouse continues to show haematopoietic activity for up to 2 weeks after birth and morphological analysis has shown that this activity becomes focused in discrete haematopoietic colonies by the end of the first week postnatal. Furthermore, each colony contains cells of one haematopoietic lineage only, that is, erythroid, myeloid or pre-B-lymphoid cells. This pattern of differentiation suggests that each colony is derived from a single committed precursor cell, which, if true, would represent the first demonstration of non-mixed haematopoietic colonies in normal development and would provide a useful system for studying the factors affecting the clonal diversity of haematopoietic stem cells and their lineage-committed progeny. Here we have analysed the haematopoietic foci in the liver of neonatal mouse chimaeras, using a newly developed ubiquitous in situ cell marker system which clearly demonstrates the clonal origin of these colonies.     

复规范形法求解非共振双Hopf分岔系统的最简规范形

提出了一种应用复规范形理论获取非共振双Hopf分岔系统最简规范形的有效方法,以简化最简规范形的求解过程.建立了复坐标下非共振双Hopf分岔系统的规范形及非线性变换,采用复数运算替代原有实数形式矩阵表示法的矩阵推导过程,获得了系统高阶关键方程的一般形式,简化了非线性变换的表达式,并且由此推导出了此类系统的最简规范形表达式.所附算例验证了最简规范形理论对于简化传统规范形结果的有效性.     

Effect of Dy3+on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts

A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline phosphatase (ALP) activity measurement, mineralized function, Oil Red O stain and measurement were employed to assess the effect of Dy³⁺ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipogenic trans-differentiation of mouse primary osteoblasts (OBs). The results showed that Dy³⁺ had no effect on BMSC proliferation at concentrations of 1×10⁻⁸ and 1×10⁻⁵ mol/L, but inhibited BMSC proliferation at other concentrations. Dy³⁺ had no effect on OB proliferation at concentrations of 1×10⁻¹⁰ and 1×10⁻⁹ mol/L, but inhibited OB proliferation at other concentrations. Dy³⁺ had no effect on the osteogenic differentiation of BMSCs at concentrations of 1×10⁻⁹ and 1×10⁻⁷ mol/L, and promoted osteogenic differentiation of BMSCs at other concentrations at the 7th day. The osteogenic differentiation of BMSCs was inhibited by Dy³⁺ at concentration of 1×10⁻⁵ mol/L at the 14th day, but promoted osteogenic differentiation of BMSCs at concentrations of 1×10⁻⁹, 1×10⁻⁸, 1×10⁻⁷ and 1×10⁻⁶ mol/L with the maximal effect at concentration of 10⁻⁶ mol/L. Dy³⁺ promoted mineralized function of BMSCs at any concentration. Dy³⁺ had no effect on adipogenic differentiation of BMSCs at concentration of 1×10⁻⁷ mol/L, but inhibited adipogenic differentiation of BMSCs at other concentrations. Dy³⁺ inhibited adipocytic trans-differentiation of OBs at any concentration, suggesting that Dy³⁺ had protective effect on bone and the protective effect on bone may be mediated by modulating differentiation of BMSCs away from the adipocyte and inhibiting adipocytic trans-differentiation of OBs which may promote differentiation and mineralization of OBs. These results may be valuable for better understanding the mechanism of the effect of Dy³⁺ on pathogenesis of osteoporosis. Supported by the Foundation for Key Program of Ministry of Education of China (Grant No. 208018)     

Jxmf类解析函数正规型

文章给出Jxmf函数类的正规型的一个简单的初等的证明方法。     

如何指导师范生进行美术创作的构思

美术的创作过程 ,是美术作品产生的过程 ,也是创作者对于现实生活进行审美认识和审美表现的过程 .在目前师范美术教学中 ,教师应当积极引导学生学会如何深入观察、细心体会 ,挖掘出生活中的美 ,并且能够通过反复酝酿、提炼 ,而形成艺术化的美 ,才能开启学生创造性思维能力和创造性能力 ,从而完成学生美术创作的构思活动 .