Effect of hepatocarcinogens on rat serumpara-phenylene diamine oxidase and xanthine dehydrogenase

Résumé On a observé une diminution de lapara-phénylène-diamine-oxydase et parfois une augmentation de la xanthine déhydrogenase dans le sérum sanguin des rats traités avec quelques substances cancérigènes pour le foie.     

Effect of azo dyes on rat serum para-phenylene diamine oxidase activity

Résumé On a trouvé que les azoïques 3-méthyl- et 4-éthyl-4-diméthylaminoazobenzène, substances cancérigènes puissantes pour le foie du rat, empêchent fortement l'activité de lap-phénylènediamine oxydase du sérum sanguin. Au contraire, 4-méthyl-4-diméthylaminoazobenzène avec pouvoir cancérigène très faible n'a aucune activité. L'azoïque 3-méthyl-4-diméthylaminoazobenzène, mais pas son isomer 4-méthyl, diminue fortement le cuivre sérique.     

Naturally occurring pea diamine oxidase inhibitors obtained fromSorghum vulgare during germination

Summary During germination ofSorghum vulgare seeds, inhibitors of pea diamine oxidase appeared in the embryos. One was heat labile, dialysable and inhibited the enzyme in vitro, while another was heat stable and inhibited the enzyme synthesis when pea seeds were soaked and allowed to germinate in the extract containing the inhibitors.Acknowledgments. We thank Dr S.K. Srivastava for his helpful suggestions and access to his facilities.     

Histochemical studies of monoamine oxidase during axonal reaction

Zusammenfassung Es werden Veränderungen der MAO-Aktivität im Rückenmark oder in der Medulla oblongata von Ratten beschrieben, die durch die Sektion des N. ischiadicus oder des N. hypoglossus verursacht wurden.     

Serum para-phenylenediamine oxidase and xanthine dehydrogenase levels during liver carcinogenesis in the rat

Zusammenfassung Werden Ratten mit dem carcinogenen 3-Methyl-4-dimethylaminoazobenzol gefüttert, so nimmt die Konzentration der p-Phenylendiaminoxidase im Serum anfangs rasch ab, um am Ende der Fütterungsperiode langsam wieder auf den Normalwert anzusteigen.Eine Verminderung der Xanthindehydrogenase des Serums war nur zu Beginn des Wachstums von Lebertumoren zu beobachten. Mit Azofarbstoffen gefütterte Ratten wurden anämisch.     

Mast cell reaction during chemical skin carcinogenesis of the lizardLacerta agilis

Zusammenfassung Beschreibung der Mastzellenreaktion während der chemisch-induzierten Carcinogenese bei der EidechseLacerta agilis.     

Potentiation of histamine and inhibition of diamine oxidase by mescaline

Zusammenfassung Anhand zweier Versuchsanordnungen wird gezeigt, dass Meskalin Histaminwirkungen verstärken kann. Ausserdem vermag es die Histamin zerstörende Wirkung der Diaminooxydase zu hemmen (in 10^–5-molarer Konzentration).Es wird die Möglichkeit diskutiert, dass diese Eigenschaften mit der 4-Methoxyphenyl-äthylamin-Struktur des Meskalins in Zusammenhang stehen könnten.     

Monoacylcadaverines as substrates for both monoamine oxidase and diamine oxidase; low rates of activity

Summary Monoacetylcadaverine and monopropionylcadaverine were found to be substrates for both rat liver monoamine oxidase and hog kidney diamine oxidase, but all the K_m-values for the oxidases were very high. The amines were common substrates for type A and type B monoamine oxidase.     

Effect of an azo dye on germination and outgrowth ofBacillus megaterium spores

Riassunto È dimostrato che il violetto di genziana non interferisce nella germinazione indotta con inosina di spore diBacillus megaterium, mentre ne inibisce la crescita alle concentrazioni di 1 o più g per ml di cultura. Quando impiegato a concentrazioni comprese fra 0,4 e 0,1 g/ml è possibile mettere in evidenza non solo forme vegetative ma anche protoplasti. Quest'ultima osservazione viene messa in relazione alla attività inibente la sintesi dei precursori del cell wall da parte del violetto di genziana.     

Correlation betweenpara-phenylene diamine oxidase activity and the copper content of rat serum

Résumé Il y a une corrélation très forte entre l'activité de lapara-phénylène diamine oxydase du sérum sanguin du rat et de son taux en cuivre.     

Effect of phytic acid on diamine oxidase activity in germinating pea seeds

Summary Diamine oxidase present in the cotyledons of germinating pea seeds is induced by phytic acid but the embryo enzyme is not affected. Polyamines have no effect on phytase activity of the cotyledon or embryo.We thank Dr S. K. Srivastava for his helpful suggestions.     

Effect of phytic acid on diamine oxidase activity in germinating pea seeds.

Diamine oxidase present in the cotyledons of germinating pea seeds is induced by phytic acid but the embryo enzyme is not affected. Polyamines have no effect on phytase activity of the cotyledon or embryo.     

Intermolecular and intramolecular isotope effects in the deamination of putrescine catalyzed by diamine oxidase

The enzymatic deamination of 1,4-diaminobutane (putrescine) catalyzed by hog kidney diamine oxidase was studied with the aid of deuterium labeled substrates and mass spectrometry. An intermolecular deuterium isotope effect for the deamination of putrescine labeled with deuterium in all 4 alpha positions was observed to be 1.26. 1,4-Diaminobutane-1,1-d2 was synthesized and intramolecular isotope effects determined. The preference of diamine oxidase for the unlabeled alpha position was about 4 times greater than for the deuterated methylene. This work shows that intramolecular deuterium isotope effects are observable in enzyme systems other than cytochrome P-450.     

Intermolecular and intramolecular isotope effects in the deamination of putrescine catalyzed by diamine oxidase

Summary The enzymatic deamination of 1,4-diaminobutane (putrescine) catalyzed by hog kidney diamine oxidase was studied with the aid of deuterium labeled substrates and mass spectrometry. An intermolecular deuterium isotope effect for the deamination of putrescine labeled with deuterium in all 4 alpha positions was observed to be 1.26. 1,4-Diaminobutane-1, 1-d₂ was synthesized and intramolecular isotope effects determined. The preference of diamine oxidase for the unlabeled alpha position was about 4 times greater than for the deuterated methylene. This work shows that intramolecular deuterium isotope effects are observable in enzyme systems other than cytochrome P-450.Acknowledgment. This investigation was supported by a research grant from the National Institutes of Health, grant number NS 14017.     

The effect of parabiosis and fluid restriction on the development of azo dye induced rat liver tumors

Résumé Chez le rat, le développement des tumeurs du foie est ralenti lorsqu'on met les individus atteints en parabiose avec des individus normaux et arrêté si l'on réduit leur ration d'eau. Mais il semble que dans les deux cas, ces effets ont pour cause déterminante la diminution de la nourriture consommée.

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This research was supported by a grant from the National Science Foundation (6139) and an institutional grant from the American Cancer Society.