The chemical nature of the pig blood-group substances dissolved in the serum

Zusammenfassung In Schweineseren finden sich die Blutgruppenantigene A, Na and Nb in gelöster Form. Aus den entsprechenden Schweineseren wurden einerseits die Totallipide, andererseits die Mucoproteide extrahiert und in immunologischen Hemmungstests auf ihre Aktivität im A- und N-System untersucht. Es ergab sich, dass die determinanten Gruppen der Na- und Nb-Antigene an Mucoproteide gebunden sind, während die determinante Gruppe des A-Antigens sowohl an ein Mucoproteid als auch an ein Lipid gebunden ist.     

The human urinary gastric inhibitor displays blood-group activity

Summary The human urinary glycoprotein with gastric antisecretory activity displays a marked blood-group activity corresponding to the blood-group of the subject examined. Several studies exclude the possibility that the activity is due to contaminants.     

The human urinary gastric inhibitor displays blood-group activity.

The human urinary glycoprotein with gastric antisecretory activity displays a marked blood-group activity corresponding to the blood-group of the subject examined. Several studies exclude the possibility that the activity is due to contaminants.     

Cyanocobalamine as a support of the in vitro cell growth-promoting activity of serum proteins

Résumé La mise au point d'un milieu synthétique pour cellules d'insectes a permis de caractériser deux actions complémentaires des protéines du sérum qu'il fallait jusqu'alors ajouter au milieu de culture. La première, revient à une protection des cellules par les antiprotéases du sérum, notamment à l'occasion de leur repiquage. L'autre, objet de ce travail, établit le rôle de certaines protéines-transport, et plus particulièrement de celles servant de support à la vitamine B₁₂.     

Proteolytic activity in the serum of rabbits during anaphylaxis

Zusammenfassung Während der Anaphylaxie des Kaninchens entstehen intravaskuläre Antigen-Antikörperkomplexe, die von den Leukozyten phagozytiert werden. Die in den Lysosomen (Granula) vorhandenen proteolytischen Enzyme werden dabei freigesetzt, und im Serum nachgewiesen.     

Model of the interaction between trypsin and alpha 2-macroglobulin of porcine serum]

A comparative study of the dissociation into subunits of Porcine alpha2 M, either native or bound to trypsin (Tn), has been carried out in order to determine the modifications of the alpha2 M structure due to the formation of the Tn-alpha2 M complex. Analytical ultra-centrifugation at pH 3.5 shows that the dissociation is smaller when alpha 2 M is bound to trypsin. Electrophoresis in 4% polyacrylamide gels, in presence of 0.1% SDS, of alpha2 M and Tn-alpha2 M incubated in 1% SDS leads to the same conclusion; the enzyme must stabilize the quaternay structure of alpha2 M. In presence of SDS + beta-mercaptoethanol, only a molecular weight (M.W.) 200,000 band is revealed in electrophoresis pattern of native alpha2 M. In the case of reduced Tn-alpha2 M, some other bands of M.W. 100,000, 50,000, 30,000 appear. When trypsin is inactivated by TLCK 100,000 M.W. band is present, accompanied by the 200,000 M.W. band whose intensity is function of the alpha2 M concentration. The 100,000 M.W. band appears therefore characteristic of the formation of the complex which must imply a proteolytic cleavage in the middle of the 100,000 polypeptidic chain of alpha2 M. A model of the complex is proposed in which the enzyme forms a proteic bridge between the two halves of the alpha2 M molecule.     

Distribution of procoagulant activity in the subcellular fractions of human granulocytes

Résumé Les extraits granulocytaires racourcissent le temps de coagulation de plasmas déficients en facteurs VIII, IX et XII. Une activité paracoagulante particulièrement élevée se manifeste dans les subfractions de granulocytes contenant des lysosomes et d'autres structures cytoplasmatiques.     

The serum histaminase activity in guinea-pig pregnancy

Résumé Chez les cobayes enceintes l'activité de l'histaminase est considérablement augmentée.     

Amylase activity in laboratory animal serum and tissues

Riassunto Si è studiato il tasso amilasico nel siero di vari animali, nonchè nelle ghiandole salivari e nel fegato di ratto. L'amilasi sierica non varia significativamente nel ratto privo delle ghiandole salivari o di 2/3 del fegato.     

Distribution of protease activity in the blastula and early gastrula ofDiscoglossus pictus

Riassunto È stata determinata l'attività proteasica nelle differenti regioni della blastula e della gastrula diDiscoglossus pictus. Nella blastula non esiste una differenza di attività fra metà animale e vegetativa, quando si prenda come riferimento l'azoto citoplasmatico. Nella giovane gastrula il territorio presuntivo della corda e del sistema nervoso mostra una attività più elevata della epidermide presuntiva.     

Effect of electroconvulsive treatment on serum dopamine-beta-hydroxylase activity in man

The activity of dopamine-beta-hydroxylase was measured in the serum before and immediately after electroconvulsive treatments. No significant difference was observed, suggesting that the seizures did not cause an increase in the peripheral sympathetic tone.     

Effect of electroconvulsive treatment on serum dopamine-beta-hydroxylase activity in man

Summary The activity of dopamine-beta-hydroxylase was measured in the serum before and immediately after electroconvulsive treatments. No significant difference was observed, suggesting that the seizures did not cause an increase in the peripheral sympathetic tone.