A rapid,sensitive method for the determination of follicle-stimulating activity

Riassunto Gli Autori riportano un nuovo metodo di saggio sul topo di piccole quantità di ormone follicolo stimolante (FSH), basato sulla riduzione intravaginale del cloruro di 2,3,5-trifeniltetrazolio cloruro (TTC). È cosi possibile determinare quantitativamente in sole 30 h dosi di FSH almeno 50 volte inferiori a quelle valutabili con i metodi fino ad oggi proposti.     

A rapid method for the purification of octopine dehydrogenase for the determination of cell metabolites

Summary Unlike other NAD⁺-dependent dehydrogenases, octopine dehydrogenase was not bound by blue Sepharose. A rapid 2-step purification procedure (gel filtration on Sephadex G-100 followed by affinity chromatography on blue Sepharose) resulted in a final preparation of octopine dehydrogenase which had a sp. act. of 65 units/mg protein and was free of contaminating NAD⁺-oxidoreductases. This preparation has been used successfully for the estimation of phospho-L-arginine, L-arginine and octopine in perchloric acid extracts.Supported by a grant from the Deutsche Forschungsgemeinschaft (Ga 241/1).     

A simplified method for isocratic HPLC analysis of polyamines

Summary A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12–15 min per sample with sensitivity in the pmole range.Acknowledgments. Supported by USPHS HD-09713, EPA R811621010 and by Research Scientist Development Award DA-00006 to TAS.     

A simplified method for isocratic HPLC analysis of polyamines

A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pmole range.     

A new method for determination of α-amylase

Zusammenfassung Für die Bestimmung der enzymatischen Aktivität der-Amylase wurde ein neues Substrat synthetisiert. Die Synthese kam in der Hauptsache durch die Farbstoffbindung an wasserlösliche Stärke und die anschliessende Vernetzung sowie durch den Zusatz von Diepoxid zustande.     

A rapid method for the fractionation of avian blood cell nuclei

Summary Red blood cell nuclei from avian anaemic blood have been fractionated by rate sedimentation on discontinous sucrose gradients into fractions which can be distinguished both by their RNA synthesizing ability and by their morphology as revealed by electron microscopy.Acknowledgment. We wish to thank H. Gitay for the electron micrographs. This work was supported by grants from the University of Cape Town Research Committee and the Council for Scientific and Industrial Research.     

A new simple method for rapid acquisition of chlamydospores inCandida albicans

Riassunto Viene descritto un nuovo e semplice metodo per ottenere in un tempo molto breve (6–8 ore) e con grande riproducibilità le clamidospore dallaC. albicans.

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Acknowledgements. We wish to express our gratitude to Dr.V. Ortali of the Istituto Superiore della Sanità, Rome, for supplies and classification of most of the species used in this study.     

A rapid electrophoretic method for the separation of hydroxyproline from proline

Summary A rapid, sensitive procedure is described which uses custom made apparatus to separate radioactive imino acids. This technique provides a simple measurement of the distribution of newly synthesized collagen in different fractions.Acknowledgment. This work was supported by grants from the International Wool Secretariat.     

A rapid method for measuring the phospholipid synthetic activity of incubated lymphocytes

Summary A rapid and simple method for estimating the phospholipid synthetic activity of incubated lymphocytes by a Florisil column technique following the uptake of¹⁴C-oleic acid was established. Stimulation of phospholipid synthesis by PHA and inhibitions caused by Tween 20 or Tween 80 and heating were evaluated easily with this method.     

A rapid method for staining thin sections of vestopal W-embedded tissue for light microscopy

Riassunto Viene descritto un metodo di colorazione con tionina e blu di metilene per un controllo, rapido e semplice, al microscopio ottico, di sezioni semifine di tessuti inclusi in Vestopal W.

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This work was supported by grants from the Royal Academy of Science, Stockholm and the Foundation of Helge Ax:son Johnson Stockholm (Sweden).     

A new rapid method for collection and preparation of cell suspensions for electrom microscopy

Summary We describe a new rapid and simple method for collection and preparation of cell suspensions for electron microscopy; the cells are prefixed with glutaraldehyde in their culture medium, and are then compacted on a filter disc. Post-fixation in osmium, staining and dehydration are performed by transferring the filter disc and the cell pellet from one solution to the next. The pellet is easily separated from the filter disc just before treatment in propylene oxide. This method preserves the fine structure as well as the classical technique. Advantages are that numerous cells have the same orientation in the sections and that many samples can be taken in a very short time.Acknowledgments. The authors are indebted to Prof. H. Leclerc, Director of I.N.S.E.R.M. laboratory U 146 in which part of this work has been performed, and to Dr J.F. Dubremetz, Dr G. Prensier and Dr P.A. Trinel for their advices.     

A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate

Summary A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.     

A method for the determination of thrombocyte aggregation in circulating rat blood

Zusammenfassung Das Ausmass einer experimentell induzierten Thrombozytenaggregation im strömenden Blut wird kontinuierlich bestimmt durch Messung des Blutdrucks vor und hinter einem extrakorporal in den arteriellen Kreislauf der Ratte aufgenommenen Filter. Die Aggregation wird durch Infusion von Adenosindiphosphorsäure (ADP) vor dem Filter induziert. Die Aggregate schliessen das Filter teilweise ab, was zu Änderungen im Blutdruck vor und hinter dem Filter führt. Aus diesen Änderungen lässt sich das Mass der Aggregation ermitteln. Es wurde gefunden, dass dieses durch die ADP-Gabe bedingt wird.     

A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.

A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.     

Brain chopping: A new method for the rapid removal of newborn rat brain

Summary A new multi-bladed air-driven guillotine is described. This device removes newborn rat heads and slices them into several thin wafers, which facilitates freezing and permits easy regional dissection.Supp. in part by U. S. Public Health Service Grant. AA-01391.