Hypoosmotic shock induces a stateⅠtransition of photosynthetic apparatus in Dunaliella salina

The effects of hypoosmotic shock on state transition in darkness in Dunaliella salina were studied. When the concentration of NaCI in culture medium was dropped from 1.5 to 0.5 mol/L abruptly, the photosynthetic rate of D.salina declined, but the respiratory rate and intracellular ATP content increased in the dark. The FpsⅡ/FpsⅠ ratio at 77 K of D. salina cells exposed to hypoosmotic shock was higher than that of control cells, indicating that more excitation energy was distributed to PS Ⅱ in stressed D.salina cells upon illumination. A decrease in LHC Ⅱ phosphorylation level was also observed when D.salina was exposed to hypoosmotic shock. Thus a state Ⅰ transition of photosynthetic apparatus occurs when D.salina suffers hypoosmotic shock in darkness, which is supposed to be related to an enhancement of respiration and an increase in ATP content in stressed D.salina cells.     

Cytokinin signal transduction： Known simplicity and unknown complexity

Cytokinin plays a critical role in plant growth and development by regulating cell divisions and cell differentiation. Recent studies suggest that cytokinin signaling is presumably mediated by a two-component system analogous to those found in bacteria and fungi, which transduces an external signal via a phosphorelay from the plasma membrane-anchored receptors to downstream effectors and regulators. Moreover, cytokinin signaling is highly interactive with other pathways, and many components of the pathway appear to be functionally redundant. Proper address of these questions will be crucial for our further understanding on this important network.     

Role of acetylcholine on plant root-shoot signal transduction

The role of accetylcholine (ACh) on plant root-shoot communication was investigated using the root-split system of Vicia faba L.In the experiments,slight osmotic stress caused the decrease of ACh content in root tips and the xylem sap transported up per time unit from root tip to the shool when the water potential of the shoot was kept unchanged .It also caused the decrease of ACh content in the abaxial epidermis,The decrease was highly correlative to the changes of transpiration rate,suggesting that the decrase of ACh content probably functions as a signal to regulate stomatal behavior,The effect of osmotic stress might be mainly through the inhibition of the ACh synthesis in root tip ;thus further influences the ACh content in root tip ,xylem sap and abaxial epidermis and resulting in the changes of stomata behavior,These results provide new evdence that plants transduce positive and negative signals among roots and shoot to coordinate stomatal behavior and adapt to variable environments.     

一氧化氮的细胞内信号转导

本文从一氧化氮/环鸟苷酸途径;激活环氧化酶;调节蛋白激酶C;影响转录因子等多个方面对一氧化氮(NO)的细胞内信号转导途径进行了阐述.     

植物抗病性的分子机制和信号传导

植物抗病性的分子机制一直是植物病理学关注的焦点.近年来,国内外不少学者和实验室正在大量分离和培养与抗病有关的突变体,并且寻找和研究与抗病有关的基因和抗病机制.研究表明,在病原物与植物的相互作用、病原信号的传导和抗病性激发的过程中存在着一系列的调节因子和基因,并形成复杂的调控网络.综述了近年来国内外植物抗病性的分子研究进展,阐述了植物抗病性分子机制和信号传导.     

Involvement of Ca<Superscript>2+</Superscript>/CaM in the signal transduction of acetylcholine regulating stomatal movement

It has been known that the neurotransmitter acetylcholine (ACh) also exists in plants and is able to regulate the movement of stomata.In another aspect,Ca^2 /CaM as the second messengers have a critical role of signal transduction in stomatal guard-cell,Here we showed that Ca^2 /CaM were also involved in the ACh regulated stomatal movement,In the medium containing Ca^2 ,the Ca^2 channel blockers (NIF and Ver)and CaM inhibitors (TFP and W7 ) could neutralize the ACh induced stomatal opening,however,they are ineffective in the medium containing K^ ,Those results indicated that Ca^2 /CaM were involved in the signal transduction pathway of ACh regulating stomatal movement.     

Nitric oxide involved in signal transduction of Jasmonic acid-induced stomatal closure of Vicia faba L.

Nitric oxide (NO) and Jasmonic acid (JA) are two key signaling molecules involved in many and diverse biological pathways in plants. Growing evidence suggested that NO signaling interacts with JA signaling. In this work, Our experiment showed that NO exists in guard cell of Vicia faba L., and NO is involved in signal transduction of JAinduced stomata closuring: ( i ) JA enhances NO synthesis in guard cell; ( ii ) both JA and NO induced stomatal closure, and had dose response to their effects; ( iU ) there are synergetic correlation between JA and lower NO concentration in regulation of stomatal movement; (iV) JA-induced stomatal closure was largely prevented by 2-phenyl-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide (PTIO), a specific NO scavenger. An inhibitor of NO synthase (NOS) in mammalian cells, N^G-nitro-L-Arg-methyl eater (L-NAME) also inhibits plant NOS, repressing JA-induced NO generation and JA-induced stomatal closure. We presumed that NO mainly comes from NOS after JA treatment.     

基于DSP的信号与系统仿真实验平台的设计

提出了一种基于DSP的信号与系统仿真实验平台实现方案．这一实验平台采用了将软件和硬件有机结合来进行仿真的方法,当用户在计算机界面上进行实验操作时,实验结果波形可以在实际的示波器上显示出来．仿真实验平台的软件方面主要是编写了一个在主机上运行的用户界面,用以将仿真实验的内容完整地展示给用户,并允许用户进行实验操作．硬件方面则使用一块以DSP系统为核心的实验板与主机通信,主要功能是将用户进行的实验操作进行计算,并将结果通过仿真,在示波器上显示出来．从而使使用者获得较为真实的实验感受,对信号处理有一个更深层次的认识．     

谐波干扰下逆变器并联系统同步控制数字信号输出方法

在谐波干扰下,传统同步控制数字信号输出方法存在谐波频率偏移、波形畸变大、输出信号同步性差的弊端。为此,提出一种新的逆变器并联系统同步控制数字信号输出方法。通过分数傅里叶方法实现谐波干扰抑制。以两个逆变器并联系统向相同的负载供电为例,对逆变器并联系统的工作特性进行研究。不同逆变器并联系统通过采样同步总线中共享的信号,求出相位误差,依据获取误差对正弦基准数字信号输出进行同步控制。在同步控制的过程中添加频率控制,使得不同逆变器的频率保持在稳态频率的水平。实验结果表明,所提方法能够有效保证数字信号输出的同步性,实用性强。     

混合信号系统设计中可靠性技术的研究

从噪声免疫，功率消耗，电容耦合三个方面在理论上分析了混合信号系统中不可靠性问题产生的原因，提出了提高混合信号系统可靠性的几条原则和技术措施，并应用于９８０ＪＸ－７３２混合信号系统中，获得成功。     

脉冲频率调制光纤高压测量传输系统的研究及应用

论述了脉冲频率调制（ＰＦＭ）光纤测量系统的特点，研制了采用ＰＦＭ的光纤高压测量信号传输系统。它可用于大电流、雷电流、１．２／５０μｓ雷电冲击全波、操作冲击波和波尾截断波的测量。报导了对衰减振荡大电流的测量结果。