首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 62 毫秒
1.
用PCR-SSCP和DNA测序的方法在两个XLRP家系RPGR基因的12号和9号外显子内各发现一个未报道的突变。这两个突变引起了严重的视网膜色素变性。  相似文献   

2.
了解转录因子GATA-2基因在ANLL中的表达和突变情况,方法:采用RT-PCR检测85例ANLL病人外周血单个核细胞中GATA-2基因的表达情况,PCR产物进一步经单链构象多态性(SSCP)分析以了解基因突变情况。结果:绝大多数ANLL都表达了GATA-2基因(89.4%),SSCP分析发现一例M2型的PCR产物出现异常迁移带,核苷序列分析显示在GATA-2基因第892位的核苷酸出现点突变,即第  相似文献   

3.
中国人P型铜转运ATP酶基因突变的初步研究   总被引:2,自引:0,他引:2  
应用聚合酶链反应-单链构象多态性(PCR-SSCP)及测序等方法分析了141便WD患者ATP7B基因第7、9及14外显子的部分DNA序列改变,通过对患者DNAT下沉人DNARPCR-SSCP电泳带迁移作对比分析,发现在141例患者中第7、9及14外显子PCR扩增产物迁移异常才分别为4例、6例和40例,依据DNA物单链构聚与分子电泳迁移的关系,提示该组病人中ATP7B基因第7、9和14外显子的突变率  相似文献   

4.
用DNA合成仪合成了分别带有PstI位点和SalI位点及终止密码子的2个用于扩增hIGF-1cDNA的PCR引物.利用合成的引物,700bp长的hIGF-1cDNA模板和Taq聚合酶进行PCR扩增.扩增产物经电泳鉴定后克隆进M13mp18载体,进行核苷酸序列分析.结果显示:PCR产物含已发表的hIGF-1成熟蛋白的编码序列和5'端的PStI位点及3'端的SaiI位点及终止密码TAG.用加端PCR技术成功地扩增和改造了hIGF-1的编码序列.  相似文献   

5.
小鼠4.5S RNA逆转座子cDNA的克隆及其表达载体的构建   总被引:2,自引:2,他引:0  
利用RT-PCR方法,从小鼠肝脏组织总RNA中扩增出4.5S RNA的cDNA.此cDNA被克隆到pGEM3Zf(+)质粒,酶切鉴定并测序。然后将该序列插入以虫荧光素酶基因作了报导基因的表达载体pSVluc20的PvuⅡ位点,构建;了4.5S RNA逆转座子的表达载体pSVluc20-4.5S,并对4.5S RNA逆转座子的生物学功能进行了研究。  相似文献   

6.
通过PCR方法扩增了人胰岛素(HI)基因1.34kb的DNA片段并克隆于pUC18的SmaⅠ位点内。经DNA序列分析表明,该片段为HI基因的氨基酸编码区及3'端调控区序列。同时,应用PCR方法对牛α-乳白蛋白(BαLA)基因进行了扩增,得到了0.84kbDNA扩增片段。将其克隆于去除了EcoRI位点的pUC18SmaI位点内,经内切酶分析和DNA测序证明该片段是BαLA基因5'调控区序列。EcoR  相似文献   

7.
日本绒螯蟹线粒体DNA序列研究:Ⅰ.12SrRNA   总被引:5,自引:3,他引:2  
参考果民蚤状序列进行了日本绒螯蟹线粒体DNA的12SrRNA基因片段的引物设计、PCR扩增及序列测定,得到457bp的碱基序列,其中A、T、G、C含量分别为159bP(34.79%)、178bp(38.95%)、50bp(10.94%)、70bp(15.32%)。  相似文献   

8.
目的:分析急性髓细胞白血病(AML)中转录因子GATA-2基因的表达和突变情况。方法:应用反转录-多聚酶链反应(RT-PCR)检测85例AML的GATA-2基因表达情况。25例正常人的外周血或骨髓作为对照。结果:85例AML中76例表达了GATA-2基因。各AML亚型均见AGTA-2表达。在85例AML发现2例出现至今未报道的GATA-2基因的突变。在一例AML-M2病人发现GATA-2cDNA  相似文献   

9.
用PCR方法从鼠伤寒沙门氏菌JL65染色体DNA中扩增了trpB基因片段,克隆到pBluescriptⅡSK质粒上,并用双脱氧末端终止法测定了其核苷酸序列。JL65trpB基因和WTS.typhimurium trpB基因作比较后,发现仅有3处的核苷酸差异,其中trpB基因的100位由C突变由A,111位由G突变为A,1030位由G突变为A,导致34位氨基酸残基Arg变为Ser,344位的Gly变  相似文献   

10.
竞争性RT-PCR检测哮喘患者IL-8表达水平   总被引:2,自引:0,他引:2  
利用反向PCR法,缺失人IL-8cDNA内部bp6邓列,构建得到IL-8突变内标分子。用已知最的该内标分子,通过竞争性RT-PCR法,检测支气管哮喘患者外周血IL-8基因的表达情况,发现患者L-8mRNA的表达水平明显高于正常人对照组,这一结果初步表明,利用竞争性RT-PCR技术对支气管哮喘患者进行早期基因诊断,具有一定的可行性。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

14.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

15.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

17.
Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.  相似文献   

18.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

19.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

20.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号