固相萃取—高效液相色谱法测定对虾体内土霉素残留量

用绞碎机破碎对虾样品，加入０．１ｍｏｌ／ＬＥＤＴＡ－Ｍｃｉｌｖａｉｎｅ缓冲液匀浆，匀浆液离心后，上层清液加入ＮａＣｌ脱蛋白，再次离心，过滤，滤液经Ｓｅｐ－ｐａｃＫＣ１８固萃取小柱富集后，用高效液相色谱法分析。     

水稻液泡膜H^＋—ATPase的分离纯化及生化性质研究

用差速离心和梯度离心的方法，分离水稻液泡膜微囊，测定膜微囊ＡＴＰａｓｅ活性，用５％的ＴｒｉｔｏｎＸ－１００溶膜后上的清液，经Ｓｅｐｈａｒｏｓｅ ＣＬ－６Ｂ柱层析纯化，得到ＡＴＰａｓｅ复合物，生化性质研究结果表明，纯化前后基本相似。     

牛乳脂球膜蛋白的研究

中国黑白花奶牛鲜乳及麦洼牦牛干乳期乳腺分泌物，高速离心后得到乳脂．以０．９％ＮａＣｌ振荡洗涤３次后，分别用３ｍｏｌ／ＬＫＣｌ、０．２％ＳＤＳ和５ｍｍｏｌ／ＬＴｒｉｔｏｎＸ－１００增溶乳脂球膜蛋白，以ＰＥＧ６０００浓缩，采用ＳＤＳ－ＰＡＧＥ分离膜蛋白，结合银染色方法，获得了分子在１２～９２ｋｄ的几种膜蛋白组分。     

Co—W—P非晶合金超细微粒制备及物性表征

报道用化学还原法在水溶液中用次亚磷酸钠，氯化钴，钨酸钠反应，制备了Ｃｏ－Ｗ－Ｐ超细非晶合金微粒。研究了反应条件及钨酸钠的加入量对Ｃｏ－Ｗ－Ｐ合金组成的影响，用Ｘ－射线衍射（ＸＲＤ），差示扫描量热（ＤＳＣ）和透射电子显微镜（ＴＥＭ）等方法对微粒进行了表征。     

电刷镀Ni-P(Ce)及Ni-Cu-P(Ce)镀层的冲击磨损行为

本文利用冲击磨损实验机，扫描电镜，Ｘ射线衍射仪等仪器观察与分析了Ｎｉ－Ｐ（Ｃｅ）及Ｎｉ－Ｃｕ－Ｐ（Ｃｅ）镀层在冲击磨损过程中的磨损现象。其中Ｎｉ－Ｐ镀层的冲击磨损过程主要表现为镀层的加工硬化、片状剥落与粘着磨损。加入稀土Ｃｅ后，增加了镀层的硬度、改变了其结构，减缓了镀层加工硬化程度，并推迟了粘着磨损的发生。而Ｎｉ－Ｃｕ－Ｐ镀层的冲击磨损除了塑性变形，疲劳片状剥落外，与Ｎｉ－Ｐ（Ｃｅ）镀层不同的是还有点蚀剥落。加入稀土Ｃｅ后，改善了镀层的结构，明显提高了镀层的抗冲击磨损性能，但没有改变镀层的冲击磨损机制。实验表明，Ｎｉ－Ｃｕ－Ｐ（Ｃｅ）镀层抗冲击磨损性能远远高于Ｎｉ－Ｐ（Ｃｅ）镀层。     

硼酸根离子在（La，Ce）PO_4：Tb中的作用

（ＢＯ３）３－加入到（Ｌａ，Ｃｅ）ＰＯ４：Ｔｂ中，一方面使Ｃｅ３＋吸收峰位置随（ＢＯ３）３－加入浓度不同有规律性变化，另一方面可以提高材料的热稳定性。本文用Ｘ光电子谱测得了与后者相应的信息，讨论了Ｃｅ（３＋）、Ｃｅ（４＋）的共存，价态数比与（ＢＯ＿３）３￣－的关系，从而解释了影响材料热稳定性机制。     

电渣重熔钢钢水离心浇铸用熔渣

有衬电渣炉炼制的钢水进行离心浇注的过程中，选定ＣａＯ－ＳｉＯ２－ＣａＦ２－Ｎａ２Ｏ－Ａｌ２Ｏ３－ＭｇＯ渣系，按给定渣系成分的熔渣进行带渣离心浇注试验，试验结果表明：新渣系未对浇注钢水产生任何污染，形成的渣皮非常均匀，厚度仅在１～２ｍｍ之间，有效地防止了表面裂纹和气孔的产生。     

溴化十六烷基三甲基铵对青霉素G钠盐碱水解反应速率的影响

用紫外分光光度法测定不同浓度的溴化十六烷基三甲基胺（简称ＣＴＡＢ）对青霉素Ｇ钠盐（简称ＰＧＳ）碱水解速率常数研究发现：当ＣＴＡＢ浓度小于２．２５０×１０－３ｍｏｌ·ｄｍ－３时，随着ＣＴＡＢ浓度的增大ＰＧＳ碱水解速率减小；当ＣＴＡＢ浓度在２．２５０×１０－３～３．７５０×１０－３ｍｏｌ·ｄｍ－３之间，随着ＣＴＡＢ加入量增加，其反应速率又增大；在实验曲线后段又出现下降。从理论上对上述现象给予解释。     

铬对高锰奥氏体钢相结构的影响

研究了铬加入高锰奥氏体钢中后，原奥氏体高锰钢组织中出现的一种新相。用Ｘ射线衍射分析确定，该相为Ｆｅ－Ｃｒ超结构相，即铬元素与奥氏体形成有序固溶体。由于铬的加入，使高锰奥氏体钢强化机制发生改变。     

柱层析法纯化且人肿瘤坏死因子α及其抑瘤作用

半合成人肿瘤坏死因子ＴＮＦ－α在大肠杆菌中高表达，发酵后收集菌体经超声破碎，然后在１２０００ｒ／ｍｉｎ４℃离心１０ｍｉｎ，上清经６０℃热处理３０ｍｉｎ，离心上清用６０％饱和度的硫酸铵沉淀，再依次经过ＳｅｐｈａｄｅｘＧ－２５，ＤＥＡＥ－５２纤维素和Ｓｅｐｈａｄｅｘ－Ｇ－７５柱层析，得到的纯化ｒｈＴＮｆ－ａ比活性为２×１０＾７ｕ／ｍｇ，回收率为２４％，经过ＳＤＳ－ＰＡＧＥ电泳和ＨＰＬＣ鉴定，纯度达９５     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with