Use of indomethacin to reverse neonatal hypotension

Summary Treatment with indomethacin elevated systemic arterial pressure and pulmonary vascular resistance in hypotensive newborn goats. Indomethacin may be of value in restoration of systemic arterial pressure in stress-induced hypotension.Supported in part by Nos. NIH-HL-10834-06, NIH-T01-HL05979-02S1 and Florida Heart No. 74-AG-2.Acknowledgments. The authors wish to thankN. L. Murtha, L. Tumarkin, J. Howard andP. Eitzman for their technical assistance and Merck, Sharpe and Dohme for the gift of indomethacin. This research was conducted under the guiding principles in the care and use of animals approved by the Council of the American Physiological Society and the National Institute of Health.     

A possible correlation between reverse mutation and complementation

Résumé Les mutants présentant la complémentation intra-allélique sont probablement susceptibles aussi de mutation reversé vraie sous l'effet de l'acide nitreux.     

Functional and structural determinants of reverse operation in the pH-dependent oligopeptide transporter PepT1

The functional and structural basis of reverse operation of PepT1 has been studied in Xenopus oocytes expressing the wild-type and mutated forms of this protein. Using brief pulses from a negative holding potential, wild-type and Arg282 mutants exhibit outward currents in the presence of Gly-Gln. The reversal potential of these currents is affected by both pH and substrate concentration, confirming coupled transport in the wild type and in the mutants as well. Long-lasting voltage and current-clamp experiments show that the outward currents are only temporary, and reflect accumulation and/or depletion effects near the membrane. The ability to operate in reverse mode was confirmed in all isoforms by intracellular injection of substrate. The role of Arg282 and Asp341 in the reverse transport was also investigated using charged substrates. Positive Lys-Gly (but not Gly-Lys) showed enhanced transport currents in the Arg282 mutants. In contrast, negative Gly-Asp and Asp-Gly elicited modest currents in all isoforms.     

基于三次B样条逆向细分的自由曲线的多分辨率表示

目前国内外关于逆向细分的研究主要集中于曲面逆向细分,对大量的特定曲线细分法的逆向细分算法研究较少,对于基于逆向细分的曲线的多分辨率构造及简化也鲜有研究.针对三次B样条细分法具有几何意义明显、规则简单等特征.本文从几何角度出发,推导并给出了基于三次B样条细分的逆向细分规则,在此基础上提出了自由曲线的一种新的多分辨率表示方法,通过在对自由曲线进行逆向细分时保留细节信息,最终可以实现自由曲线的多分辨率表示,并可应用于自由曲线的简化与精确重构中.文中给出了曲线的多分辨率表示、简化和重构的例子.该方法几何意义明显,易于编程实现.实验表明应用该逆向细分法得到的简化曲线能够更明显地反映原曲线的变化趋势.本文方法在构造分解矩阵和重构矩阵方面较以往的某些方法简单,并且在分解和重构曲线时的计算量相较于以往的方法较少.     

Steady-state kinetic studies with the polysulfonate U-9843, an HIV reverse transcriptase inhibitor

The tetramer of ethylenesulfonic acid (U-9843) is a potent inhibitor of HIV-1 RT^* and possesses excellent antiviral activity at nontoxic doses in HIV-1 infected lymphocytes grown in tissue culture. Kinetic studies of the HIV-1 RT-catalyzed RNA-directed DNA polymerase activity were carried out in order to determine if the inhibitor interacts with the template: primer or the deoxyribonucleotide triphosphate (dNTP) binding sites of the polymerase. Michaelis-Menten kinetics, which are based on the establishment of a rapid equilibrium between the enzyme and its substrates, proved inadequate for the analysis of the experimental data. The data were thus analyzed using steady-state Briggs-Haldane kinetics assuming that the template:primer binds to the enzyme first, followed by the binding of the dNTP and that the polymerase is a processive enzyme. Based on these assumptions, a velocity equation was derived which allows the calculation of all the specific forward and backward rate constants for the reactions occurring between the enzyme, its substrates and the inhibitor. The calculated rate constants are in agreement with this model and the results indicated that U-9843 acts as a noncompetitive inhibitor with respect to both the template:primer and dNTP binding sites. Hence, U-9843 exhibits the same binding affinity for the free enzyme as for the enzyme-substrate complexes and must inhibit the RT polymerase by interacting with a site distinct from the substrate binding sites. Thus, U-9843 appears to impair an event occurring after the formation of the enzyme-substrate complexes, which involves either an event leading up to the formation of the phosphoester bond, the formation of the ester bond itself or translocation of the enzyme relative to its template:primer following the formation of the ester bond.     

Inhibition of erythrocyte ATPase activity by aclacinomycin and reverse effects of ascorbate on ATPase activity

We studied the effect of aclacinomycin on human erythrocyte membrane enzymes. Aclacinomycin inhibited ATPase, including Na-K-dependent ATPase, ouabain insensitive ATPase and Ca-ATPase. However acetylcholinesterase was not inhibited by aclacinomycin. The ATPase activities were not inhibited by aclacinomycin if ascorbate was added to the incubation mixture. However other reducing agents, alpha-tocopherol, superoxide dismutase and catalase had no effect on ATPase activity. Ascorbate may protect membrane proteins and lipids from peroxidate damage.     

Levels of triiodothyronine and reverse triiodothyronine in the thyroid glands of man and swine at different stages of development]

3,5,3'-triiodothyronine (T3) and 3,3',5'-triiodothyronine (rT3) were measured by radioimmunoassay in saline extracts of neonates and human adult thyroid tissues and of fetuses, Piglets and adult Swine thyroid tissues. In all these extracts, T3 content was higher than rT3 content whatever the period of development. Both triiodoamino acids represent a small percentage of the iodinated protein in thyroid tissues.     

Presence in immunostimulated cells of an RNA molecule utilizable as template for reverse transcriptase of avian myeloblastosis virus (AMV)]

Cytoplasmic RNA extracted from antigen stimulated immunocompetant cells is transcribed in vitro into DNA by the RNA directed DNA polymerase from avian myeloblastosis virus, in the absence of any added primer. Cytoplasmic RNA from other organs of the same animal, from non-stimulated immunocompetent cells, or from cells in tissue culture is not transcribed in the absence of exogenous primer.