共查询到20条相似文献,搜索用时 15 毫秒
1.
S el-Mofty 《Experientia》1985,41(12):1548-1549
The secretory activity of the main excretory duct of rat submaxillary gland was investigated by the technique of luminal perfusion. Immunologic studies of the perfusate revealed the presence of serum antigens and the absence of intrinsic submaxillary gland antigens. It is suggested that the submaxillary duct permits passive transport of serum proteins to saliva from serum. 相似文献
2.
The enzymes which were extracted by autodigestion from the microsomal fractions of the pig kidney, liver and submaxillary gland and from the serum showed an immunochemical identity by a double immunodiffusion test. But the kidney enzyme had a different pI-value from the pI-values of the enzymes of other organs. 相似文献
3.
Substrate specificity of X-prolyl dipeptidyl-aminopeptidase (dipeptidyl aminopeptidase IV) was examined by using newly synthesized 8 chromogenic substrates, X-Y-p-nitroanilides. Homogeneous enzyme from human submaxillary gland hydrolyzed glycylproline p-nitroanilide almost specifically, except alanylalanine p-nitroanilide which had 11% activity. 相似文献
4.
T. Kato T. Nagatsu T. Kimura S. Sakakibara 《Cellular and molecular life sciences : CMLS》1978,34(3):319-320
Summary Substrate specificity of X-prolyl dipeptidyl-aminopeptidase (dipeptidyl aminopeptidase IV) was examined by using newly synthesized 8 chromogenic substrates, X-Y-p-nitroanilides. Homogeneous enzyme from human submaxillary gland hydrolyzed glycylproline p-nitroanilide almost specifically, except alanylalanine p-nitroanilide which had 11% activity. 相似文献
5.
K. M. Fukasawa K. Fukasawa M. Harada 《Cellular and molecular life sciences : CMLS》1979,35(9):1142-1143
Summary The enzymes which were extracted by autodigestion from the microsomal fractions of the pig kidney, liver and submaxillary gland and from the serum showed an immunochemical identity by a double immunodiffusion test. But the kidney enzyme had a different pI-value from the pI-values of the enzymes of other organs.This investigation was supported by a grant from the Ministry of Education, Japan to K. M. F. We would like to thank Dr Toshiharu Nagatsu (Department of Life Chemistry, Graduate School at Nagatsuta, Tokyo Institute of Technology, Yokohama, Japan) for helpful suggestions, Mr Moritoshi Sato (Matsumoto Meat Inspection Station, Matsumoto, Japan) for their generous supply of pig organs and Ajinomoto Co. Inc., Tokyo, Japan, for the gift of Gly-Pro-p-nitroanilide tosylate. Technical assistance of Miss K. Yanagisawa is gratefully acknowledged. 相似文献
6.
Summary The primary alcohol group on the carbon 6 of terminal galactosyl and N-acetylgalactosaminyl moieties of glycoproteins can be oxidized to an aldehyde by treatment with galactose oxidase. By reacting these aldehyde groups with14C-labeled sodium cyanide,14C-labeled cyanohydrin derivatives were obtained. Similarly, reduction of these aldehyde groups with tritiated sodium borohydride following standard procedures, yields3H-labeled glycoproteins.14C- and3H-labeled derivatives of asialofetuin and asialo ovine submaxillary mucin with high specific radioactivities were prepared using these procedures. Mixtures containing microgram amounts of14C- and3H-labeled glycoproteins were subjected to column chromatography and gradient ultracentrifugation and the position of the individual glycoproteins was determined by simultaneous counting for14C and3H. These experiments demonstrate the usefulness of this approach for comparative analytical studies using biological specimens available in minute quantities. 相似文献
7.
K. Hatakeyama M. Hiramatsu N. Minami 《Cellular and molecular life sciences : CMLS》1980,36(10):1219-1220
Summary Removal of the submaxillary gland (SMG) from male but not female mice caused a suppressed immune response to sheep red blood cells. Administration of a SMG saline extract from male mice to SMG-ectomized males restored the suppressed response to control levels. This suggests that the male mouse SMG contains a factor(s), possibly of an endocrine nature, capable of influencing cells involved in an immune response. 相似文献
8.
E Paulo 《Experientia》1984,40(4):396-397
Relatively high progestagen levels were found in female rat submaxillary glands, with a maximum in the proestrus stage at 22 h and 2 minima; in proestrus between 10 and 14 h, and in estrus at 14 h. Estrogen and androgen concentrations in the gland were undetectable during most of the cycle except in the proestrus stage, when the highest level was determined at 14 h for estrogens and at 17 h for androgens. 相似文献
9.
Y Lutz-Ostertag 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,284(23):2407-2410
Some androgenic hormones injected into the vitellus of quail (8 and 10 days old) or chick eggs (10 and 12 days of incubation) inhibit the left Müllerian duct development and mainly that of the shell gland. They slow down or stop the right duct involution. After these effects, histo-pathogenic disturbances, affect them. 相似文献
10.
S Kanamura 《Experientia》1978,34(4):517-518
Reaggregated striated duct cells of rat submandibular gland formed deep tubular invaginations of the cell membrane, i.e. intracellular canaliculi, which are not present in the original tissue, after 24 h of the stationary culture. 相似文献
11.
Summary In the dog and guinea-pig submandibular glands kallikrein seems to be present in the striated duct cells. Following sympathetic nerve and in vivo isoproterenol stimulation of the dog and guinea-pig submandibular gland respectively, there is a reduction of kallikrein concentration. Ultrastructurally this reduction corresponds to the decrease of straited duct secretory granules in both species. Parasympathetic stimulation also causes some release of kallikrein from both species.Supported by Dean's M.R.C. grant to the College of Dentistry. 相似文献
12.
A novel agglutinin with specificity for sialic acid sequence of sugars in thyroglobulin is identified in the hemolymph of Scylla serrata. The physico-chemical characteristics of its binding affinity, such as pH and temperature optima, and cationic requirements are defined. N-glycolyl neuraminic acid (NeuGc) (at 0.6 mM), in contrast to N-acetyl neuraminic acid (NeuAc) (at greater than 5.0 mM), is the potent inhibitor of hemagglutination. Bovine and porcine thyroglobulins containing NeuGc, inhibited the agglutination. NeuGc-acid glycoprotein fraction (bovine) but not NeuAc-acid glycoprotein fraction (human) inhibited the hemagglutination. The inability of other NeuGc-glycoproteins (bovine submaxillary mucin) to inhibit the agglutination suggests that the agglutinin may also recognize glycosidic linkage associated with NeuGc. The potential of the agglutinin in identifying NeuGc containing human tumor associated antigens is discussed. 相似文献
13.
A novel agglutinin with specificity for sialic acid sequence of sugars in thyroglobulin is identified in the hemolymph ofScylla serrata. The physico-chemical characteristics of its binding affinity, such as pH and temperature optima, and cationic requirements are defined. N-glycolyl neuraminic acid (NeuGc) (at 0.6 mM), in contrast to N-acetyl neuraminic acid (NeuAc) (at >5.0 mM), is the potent inhibitor of hemagglutination. Bovine and porcine thyroglobulins containing NeuGc, inhibited the agglutination. NeuGc-acid glycoprotein fraction (bovine) but not NeuAc-acid glycoprotein fraction (human) inhibited the hemagglutination. The inability of other NeuGc-glycoproteins (bovine submaxillary mucin) to inhibit the agglutination suggests that the agglutinin may also recognize glycosidic linkage associated with NeuGc. The potential of the agglutinin in identifying NeuGc containing human tumor associated antigens is discussed. 相似文献
14.
H. Z. Levinson A. R. Levinson G. E. Kahn K. Schäfer 《Cellular and molecular life sciences : CMLS》1983,39(10):1095-1097
Summary A pheromone-producing gland was discovered in the second abdominal segment of virgin female tobacco beetles,Lasioderma serricorne (Fabricius). The gland duct extends to an orifice below the genital pore and is supported by a rigid invagination of the integument. Hexane extracts of intact pheromone glands were found attractive to male tobacco beetles and also induced high receptor potentials in the olfactory sensilla of the antennae of maleL. serricorne. A surface extract of virgin females proved to be significantly more attractive than an extract of pheromone glands. 相似文献
15.
A. Scaloni S. Paolini A. Brandazza M. Fantacci C. Bottiglieri S. Marchese A. Navarrini C. Fini L. Ferrara P. Pelosi 《Cellular and molecular life sciences : CMLS》2001,58(5-6):823-834
Two distinct classes of lipocalin isoforms (OBP-IIs and OBP-IIIs) were purified and identified from porcine nasal mucosa of male and female individuals. Using primers designed on their N-terminal sequence, the complete primary structures of the mature polypeptides were determined. Mass spectrometry analysis confirmed the identity of the cDNA-derived sequences and provided information regarding their post-translational modifications. These species strongly resemble a lipocalin expressed by von Ebner's gland and salivary lipocalins carrying sex-specific pheromones secreted only by the boar's submaxillary glands. Both OBP-IIs and OBP-IIIs present two cysteines paired in a disulphide bond; the remaining residues occur in a reduced form. In addition, OBP-IIIs are heavily glycosylated and markedly different in their glycan moiety from the salivary lipocalins. A three-dimensional model is proposed based on protein species with known structure. Like salivary lipocalins, OBP-IIIs bind a number of odorant molecules, with highest affinity for the specific pheromone 5alpha-androst-16-en-3-one. The high similarity between OBPs from the nasal area and lipocalins from secretory glands suggests a common function in binding the same pheromonal ligands, the latter carrying chemical messages into the environment the former delivering them to specific receptors. 相似文献
16.
The existence of antigens shared in common by T. cruzi and heart muscle cells is suggested by the presence of antibodies binding to the parasite surface in the serum of mice with autoimmune myocarditis induced by immunization with syngenic heart antigens. 相似文献
17.
M Pla J M Colombani 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1979,289(5):477-480
An anti-Ia immune serum (A . TL anti-A . TH) directed to the antigens of the stimulating cell, blocked the MLR-II. A significant correlation was observed between the anti-Ia reactivity of the immune serum, studied against a panel of eleven original H-2 haplotypes and the reactivity against the same panel of the in vitro primed responding cells (A . TL anti-A . TH). These results confirm the hypothesis that Ia antigens are the structures stimulating the MLR-II. 相似文献
18.
Summary The effect of pinealectomy on the circadian periodicity in serum gonadotropins was investigated in adult male rats. Pinealectomy resulted in an elevation of the serum FSH concentration 7 days after the operation. A 4-fold increase in serum FSH over 24 h with no concommitant increase in serum LH following pinealectomy suggests that the control of FSH secretion could be mediated via the pineal gland. 相似文献
19.
The effect of pinealectomy on the circadian periodicity in serum gonadotropins was investigated in adult male rats. Pinealectomy resulted in an elevation of the serum FSH concentration 7 days after the operation. A 4-fold increase in serum FSH over 24 h with no concommitant increase in serum LH following pinealectomy suggests that the control of FSH secretion could be mediated via the pineal gland. 相似文献
20.
J. G. Chambó P. M. Cabeza Meckert R. P. Laguens 《Cellular and molecular life sciences : CMLS》1990,46(9):977-979
Summary The existence of antigens shared in common byT. cruzi and heart muscle cells is suggested by the presence of antibodies binding to the parasite surface in the serum of mice with autoimmune myocarditis induced by immunization with syngeneic heart antigens. 相似文献