共查询到20条相似文献,搜索用时 15 毫秒
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Histone acetylation and disease 总被引:18,自引:0,他引:18
S. Timmermann H. Lehrmann A. Polesskaya A. Harel-Bellan 《Cellular and molecular life sciences : CMLS》2001,58(5-6):728-736
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We report that histones H2A and H2B possess gonadotrophin-releasing activity in vitro and assess the signal transduction
pathways involved in these effects. Perifused and incubated rat anterior pituitary (AP) cells were used, and luteinizing hormone
(LH) and follicle stimulating hormone (FSH) were measured by RIA. Perifusion of cells with histone H2A (30 μM) or histone
H2B (30 μM), markedly stimulated LH release but failed to elicit any FSH response. Cells incubated with 6 or 30 μM histone
H2A showed a dose- and time-dependent stimulatory effect on both LH and FSH release which was blocked by 1 μM peptide MB35,
an 86–120 amino acid fragment of histone H2A. Incubation of pituitary cells with gonadotrophin-releasing hormone (GnRH) and
histones H2A or H2B showed a stimulatory effect on LH and FSH release which was similar to the sum of the separate effects.
Trifluoperazine, as well as ethylene glycol bis(b-aminoethyl ether) N,N,N′,N′-tetraacetic acid (EGTA), alone or in the presence
of the calcium ionophore A23187, significantly reduced the response of AP cells to histones. Various cyclic adenosine monophosphate
(cAMP) enhancers had no effect on histone-stimulated release of gonadotrophins in incubated AP cells. Our results confirm
previous evidence that histones may act as hypophysiotrophic signals. Calcium- and diacylglycerol-associated pathways, but
not cAMP, appear to participate in these effects.
Received 11 August 1997; received after revision 20 January 1998; accepted 26 January 1998 相似文献
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D. Robinette S. Wada T. Arroll M. G. Levy W. L. Miller E. J. Noga 《Cellular and molecular life sciences : CMLS》1998,54(5):467-475
Three antibacterial proteins were isolated from acid extracts of channel catfish (Ictalurus punctatus) skin by cation exchange chromatography and reverse-phase high-pressure liquid chromatography. The molecular masses of the
proteins were 15.5, 15.5 and 30 kD as determined by SDS-polyacrylamide gel electrophoresis. Mass spectrometry, amino acid
composition and amino acid sequence data suggest that the most abundant protein is closely related to histone H2B. The H2B-like
protein was inhibitory to Aeromonas hydrophila and Saprolegnia spp., which are important bacterial and fungal pathogens of fish. These findings suggest that histones may be important defensive
molecules in fish.
Received 22 December 1997; received after revision 5 March 1998; accepted 5 March 1998 相似文献
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K. Sandman S. L. Pereira J. N. Reeve 《Cellular and molecular life sciences : CMLS》1998,54(12):1350-1364
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Juste Wesche Sarah Kühn Benedikt M. Kessler Maayan Salton Alexander Wolf 《Cellular and molecular life sciences : CMLS》2017,74(18):3305-3315
Arginine methylation of histones is one mechanism of epigenetic regulation in eukaryotic cells. Methylarginines can also be found in non-histone proteins involved in various different processes in a cell. An enzyme family of nine protein arginine methyltransferases catalyses the addition of methyl groups on arginines of histone and non-histone proteins, resulting in either mono- or dimethylated-arginine residues. The reversibility of histone modifications is an essential feature of epigenetic regulation to respond to changes in environmental factors, signalling events, or metabolic alterations. Prominent histone modifications like lysine acetylation and lysine methylation are reversible. Enzyme family pairs have been identified, with each pair of lysine acetyltransferases/deacetylases and lysine methyltransferases/demethylases operating complementarily to generate or erase lysine modifications. Several analyses also indicate a reversible nature of arginine methylation, but the enzymes facilitating direct removal of methyl moieties from arginine residues in proteins have been discussed controversially. Differing reports have been seen for initially characterized putative candidates, like peptidyl arginine deiminase 4 or Jumonji-domain containing protein 6. Here, we review the most recent cellular, biochemical, and mass spectrometry work on arginine methylation and its reversible nature with a special focus on putative arginine demethylases, including the enzyme superfamily of Fe(II) and 2-oxoglutarate-dependent oxygenases. 相似文献
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Emerging connections between DNA methylation and histone acetylation 总被引:18,自引:0,他引:18
Modifications of both DNA and chromatin can affect gene expression and lead to gene silencing. Evidence of links between
DNA methylation and histone hypoacetylation is accumulating. Several proteins that specifically bind to methylated DNA are
associated with complexes that include histone deacetylases (HDACs). In addition, DNA methyltransferases of mammals appear
to interact with HDACs. Experiments with animal cells have shown that HDACs are responsible for part of the repressive effect
of DNA methylation. Evidence was found in Neurospora that protein acetylation can in some cases affect DNA methylation. The available data suggest that the roles of DNA methylation
and histone hypoacetylation, and their relationship with each other, can vary, even within an organism. Some open questions
in this emerging field that should be answered in the near future are discussed. 相似文献
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Ignacio Campillo-Marcos Pedro A. Lazo 《Cellular and molecular life sciences : CMLS》2018,75(13):2375-2388
DNA damage causes a local distortion of chromatin that triggers the sequential processes that participate in specific DNA repair mechanisms. This initiation of the repair response requires the involvement of a protein whose activity can be regulated by histones. Kinases are candidates to regulate and coordinate the connection between a locally altered chromatin and the response initiating signals that lead to identification of the type of lesion and the sequential steps required in specific DNA damage responses (DDR). This initiating kinase must be located in chromatin, and be activated independently of the type of DNA damage. We review the contribution of the Ser-Thr vaccinia-related kinase 1 (VRK1) chromatin kinase as a new player in the signaling of DNA damage responses, at chromatin and cellular levels, and its potential as a new therapeutic target in oncology. VRK1 is involved in the regulation of histone modifications, such as histone phosphorylation and acetylation, and in the formation of γH2AX, NBS1 and 53BP1 foci induced in DDR. Induction of DNA damage by chemotherapy or radiation is a mainstay of cancer treatment. Therefore, novel treatments can be targeted to proteins implicated in the regulation of DDR, rather than by directly causing DNA damage. 相似文献
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Histone deacetylase controls adult stem cell aging by balancing the expression of polycomb genes and jumonji domain containing 3 总被引:1,自引:1,他引:0
Ji-Won Jung Seunghee Lee Min-Soo Seo Sang-Bum Park Andreas Kurtz Soo-Kyung Kang Kyung-Sun Kang 《Cellular and molecular life sciences : CMLS》2010,67(7):1165-1176