Reaction of pulmonary macrophages to the presence of foreign protein material in the alveoli during metabolic acidosis

Zusammenfassung Tierexperimentell wurde festgestellt, dass die metabolische Azidose keinen Einfluss auf die Fähigkeit der Alveolarzellen hat, in die Alveolen auszuwandern.     

The cytochemical demonstration of dehydrogenases and oxidases in the cells of fungi

Zusammenfassung Die Ergebnisse der bisherigen Untersuchungen zum zytochemischen Nachweis verschiedener Dehydrogenasen und Oxydasen in den Zellen von Pilzen (Neurospora crassa, Oospora lactis, Saccharomyces cerevisiae u.a.) werden beschrieben.     

Interaction of polyamines or their precursors with the calcium-controlled secretion of peroxidase by sugarbeet cells

Summary Three polyamines tested (cadaverine, spermidine and spermine) and their 2 precursors (the amino acids arginine and ornithine) inhibit the Ca²⁺-mediated secretion of peroxidases by sugarbeet cells in suspension culture at concentrations ranging from 10^–15 to 10^–5M. In the absence of exogenous Ca²⁺, spermine added at higher concentrations mimics the activatory effect of Ca²⁺, the other polyamines being without effect.Supported by the Belgian FRFC (grant No. 2.9009.75 to T.G.) and the Swiss National Foundation for Scientific Research (grant No. 3.140-0.81 to C.P. and H.G.).     

A comparative study of nuclear proteins in chick embryo cells and their primary and secondary fibrobasts in culture

Zusammenfassung Das elektrophoretische Muster von Histonen und sauren Zellkernproteinen von Hühnerembryonen und deren primären und sekundären Fibroblastzellen wurde qualitativ und quantitativ verglichen. Die Zellkernproteine der verschiedenen Zelltypen erwiesen sich qualitativ als identisch, die relative quantitative Verteilung der Kernproteine war jedoch leicht verschieden.     

Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

Summary Rumen epithelial cells (REC) were incubated in the presence of various concentrations of butyrate or insulin or with both of them, to obtain information on their effect on the DNA synthesis of cultured cells. The 24-h values of³H-thymidine incorporation into cellular DNA were measured in the presence of butyrate, insulin or butyrate plus insulin. While butyrate reduced DNA synthesis, insulin produced an increase over the control. Combined butyrate plus insulin treatment influenced the incorporation of label in accordance with the relative proportion of these two substances.     

Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

Rumen epithelial cells (REC) were incubated in the presence of various concentrations of butyrate or insulin or with both of them, to obtain information on their effect on the DNA synthesis of cultured cells. The 24-h values of 3H-thymidine incorporation into cellular DNA were measured in the presence of butyrate, insulin or butyrate plus insulin. While butyrate reduced DNA synthesis, insulin produced an increase over the control. Combined butyrate plus insulin treatment influenced the incorporation of label in accordance with the relative proportion of these two substances.     

Rat pancreatic islet cells in primary culture: occurrence of giant cells amenable to patch clamping

Summary Neonatal and adult rat pancreatic islet cells were maintained in dissociated cell culture for up to three weeks. The unexpected occurrence of giant (40–50 m) cells was noted, some of which reacted positively to an insulin antiserum, indicating the presence of insulin. The giant cells were amenable to study using the extracellular patch clamp technique, which was used to demonstrate a population of membrane channels gating outwardly directed current in these cells.     

Rat pancreatic islet cells in primary culture: occurrence of giant cells amenable to patch clamping

Neonatal and adult rat pancreatic islet cells were maintained in dissociated cell culture for up to three weeks. The unexpected occurrence of giant (40-50 micron) cells was noted, some of which reacted positively to an insulin antiserum, indicating the presence of insulin. The giant cells were amenable to study using the extracellular patch clamp technique, which was used to demonstrate a population of membrane channels gating outwardly directed current in these cells.     

Differentiation of chick embryo brain cells in culture

Résumé Des cellules, encore à un stade indifférencié, dissociées d'hémisphères cérébraux d'embryon de Poulet ont formé, en culture, des fibres nerveuses. La plupart des cellules se différencient en neuroblastes unipolaire et bipolaire; certaines évoluent vers le type multipolaire sans contact direct avec des cellules gliales.

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Chargée de Recherche au CNRS.     

Synthesis and transport of cholesterol in aortic cells: Radioisotopic studies in cell culture

Zusammenfassung Synthese und Transport von Cholesterin in Hühner-Aortazellen wurden untersucht: 1. grösste Inkorporationsrate von C¹⁴-Acetat in der Sterin-Fraktion, 2. verminderte Aufnahme von C¹⁴-Cholesterin in Aortazellen nach ihrer Inkubation mit lipämischem Menschenserum. Der Eintritt von H³-Cholesterin in das Zytoplasma der Aortazellen von Affen wurde autoradiographisch festgestellt.     

Heart cells in culture: A simple method for increasing the proportion of myoblasts

Résumé La plupart des cellules mésenchymateuses présentes dans une suspension cellulaire obtenue par trypsination de curs de rats nouveau-nés s'attachent au récipient de culture avant les myoblastes. Cette propriété est utilisée pour obtenir des cultures confluentes contenant une proportion élevée de myoblastes actifs.

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This work was supported by the grant Nr. 5050.3 from the Swiss National Foundation for Scientific Research.