聚乙烯醇-透明质酸-胶原组织工程支架研究

以聚乙烯醇(PVA)、透明质酸(HA)和胶原蛋白(Col)为组分,构建组织工程三维多孔支架.将聚乙烯醇分别与不同质量的胶原和透明质酸复合,测定复合材料的含水率和膨胀率.扫描电镜观察材料横截面的表面形态.结果是不同分子质量的PVA与不同配比的Col和HA复合,得到的复合支架材料含水率为57.97%～81.26%,膨胀率为150.00%～459.20%.不同配比的复合支架材料具有不同的内部孔洞结构.结果表明,PVA-HA-Col复合支架材料具有较高的含水率和膨胀率,内部孔洞网络丰富,可作为组织工程支架材料.     

角膜组织工程支架壳聚糖-胶原复合膜的性能

制备了壳聚糖-胶原角膜组织工程支架,考察了复合膜支架的光学性能、力学强度、结晶性、吸水率、亲水性、微观形貌及细胞亲和性,发现支架内胶原与壳聚糖两种分子间存在较强的相互作用,且具有良好的相容性,支架表面平滑均一,内部呈现层状有序结构．壳聚糖-胶原复合膜具有优异的透光率和适宜的湿态力学强度．细胞培养实验中,人角膜缘上皮细胞能在支架上较好地粘附和增殖分化,显示复合膜支架具有良好的细胞亲和性．结果表明壳聚糖-胶原复合膜有望成为一种性能良好的角膜组织工程支架．     

牛血清白蛋白改性壳聚糖纤维及其细胞亲和性研究

采用牛血清白蛋白对壳聚糖纤维进行化学改性，通过扫描电镜和含水量测定对改性的牛血清白蛋白-壳聚糖纤维进行表征．并对改性材料进行细胞相容性和细胞亲和性测定与评价．结果表明：牛血清白蛋白-壳聚糖纤维表面有明显附着物，纤维略微变粗；含水量增加到93．49％(质量分数)；细胞在改性克聚糖纤维上的形态无异常，粘附、生长、增殖情况良好；细胞毒性试验结果呈阴性．该改性壳聚糖纤维具有良好的生物学特性，能够应用于器官组织工程及生物医药领域．     

胶原-壳聚糖复合支架体外三维培养神经干细胞

采用冷冻干燥法制备了胶原-壳聚糖复合支架并通过层粘连蛋白（LN）进行结构修饰,测定其物理性能特征,建立了胎鼠海马神经干细胞（NSCs）的三维培养体系,同时考察了NSCs与胶原-壳聚糖复合支架的生物相容性．孔径、孔隙率、吸水率、降解率等参数的比较表明,体积比为7：3的复合支架更适合于体外细胞培养的要求,并且NSCs在经LN修饰后支架材料上的接种率得到明显提高;激光共聚焦显微镜及扫描电镜观察表明,NSCs能够在胶原-壳聚糖复合支架内良好地生长、增殖及分化．这些结果为NSCs的进一步临床移植应用,以及治疗神经系统疾病的新药筛选和机理研究等奠定了坚实的实验基础．     

聚乙烯醇—海藻酸钙复合材料制备及性质

目的：以聚乙烯烯醇（PVA）和海藻酸钠为主要组分,通过对两种聚合物的分步交联,获得具有交际结构的PVA－海藻酸钙复合材料,方法：探讨了PVA和海藻酸钠不同配比,交联剂氯化钙不同浓度对材料性能的影响,从中选择出了PVA与海藻酸钠的较佳配比为4：1和交联剂氯化钙的适宜浓度为2％,用原子吸收光谱测定了材料中钙的含量,用扫描电镜观察材料的组织形态,结果：所制得的PVA－海灌酸钙交联复合物,外观均一,光洁平滑,柔韧具弹性,呈乳白色,湿态下扯断伸长率为35％,拉伸强度为20MPa,含水率为77％。结论;PVA和海藻酸钠通过交联反应形成PVA－海藻酸钙复合物后,两者的玻璃化温度向彼此接近的方向发生了内移,在一定程度上发生了分子水平的互溶,交际反应增加了两者之间的互溶性。     

膨润土改性天然粘土防渗材料的研究

膨润土具有很好的防渗和吸附性能．在华北黄土和华南红土中加入一定量的膨润土，以增强衬层材料的防渗性能．研究了膨润土加入量对土样抗压强度和压实土样渗透系数的影响，压实含水率、压实干密度等因素对压实粘土渗透性能的影响．实验原理为Darcy定理．实验结果表明：对于黄土，膨润土合量为6％—7％时，抗压强度最大．选择大于强度最优含水率2％的压实含水率有利于提高黄土及其改性粘土的防渗功能．对于红土，加入8％的膨润土对其抗压强度有很大的改善，抗渗性能有着较大的提高，渗透系数只相当于原土的1／30．选用24％左右的压实含水率可以使红土(及其改性粘土)的抗压性能与防渗性能同时优化．     

壳聚糖超滤膜的孔结构研究

采用凝固浴凝胶工艺处理壳聚糖超滤膜，在制膜过程中通过改变凝胶工艺参数来调节膜孔结构．经凝固浴处理后，壳聚糖超滤膜对酸性红B的截留率提高了83．7％；不同凝固浴的温度、凝固剂的浓度、凝固时间等处理参数对壳聚糖超滤膜的孔结构有一定的影响，采用浓度为0．25～1．0mol／L的NaOH溶液为凝固浴，凝固浴温5～15℃，凝固时间为1．5min，可使壳聚糖膜获得良好的的分离效率和孔结构．     

鲨软骨粘多糖的酶法提取及组分分析

通过正交实验法分别研究单酶法提取次数和双酶法不同提取方式对鲨软骨粘多糖提取率的影响,从而获得胰蛋白酶和／或木瓜蛋白酶水解鲨软骨提取粘多糖的最佳工艺：并采用乙酸纤维素薄膜电泳和琼脂糖凝胶电泳分析不同提取方法获得的粘多糖的组分．实验结果表明：双酶水解方式比单酶水解方式的软骨降解率和多糖提取率要高;在双酶水解方式中,木-胰方式比胰-木方式能更有效地降解软骨,并且能获得更高的粘多糖提取率;而木瓜蛋白酶法提取的粘多糖纯度比胰蛋白酶法高．     

深冲铝板坯冷轧显微组织、织构与力学性能

研究了不同冷轧工艺参数的深冲铝板坯（wA1＞99．75％）的显微组织、织构，测定了不同方向的力学性能；由D－500全自动X射线衍射仪建测极图，按Bunge的方法计算取向分布函数（ODF）；通过透射电镜观察变形显微结构．结合冷轧变形亚组织结构和织构特征，讨论了位错胞结构与板坯塑性变形和力学性能的关系，以及对板坯塑性各向异性的影响，预测材料深冲性能．分析认为，材料力学性能决定于变形组织结构和织构，随冷变形量增大，位错缠结胞变形并发生取向排列形成显微带，使材料产生力学性能各向异性及加工硬化．本研究显微组织和织构特征及力学性能分析结果表明，接近50％冷轧变形量的板坯中变形亚组织均匀、多种织构混存，有利于降低材料深冲制耳率．并经工业制罐试验验证．     

壳聚糖与胶原或海藻酸复合物海绵的制备以及人胎儿皮肤成纤维细胞在 …

壳聚糖与胶原或海藻酸形成高分子离子复合物后，采用浇铸／冷冻干燥技术，制备了壳聚糖复合物海绵。表征不同组成复合物海绵的亲水性和形貌，发现加入胶原或海藻酸可增加海绵的吸水性和保水性，并有助于海绵中形成大孔结构。海在ｐＨ７．４的磷酸盐缓冲液中用溶菌酸进行体外降解，复合物海绵的降解速率比单纯的壳聚糖海绵稍快．在海绵中进行人胎儿皮肤成纤维细胞的培养，发现细胞在复合物海绵中的生长增殖优于单纯的壳聚糖海绵，而且     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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