High-frequency induction in vivo of mouse leukemia in AKR strain by 5-azacytidine and 5-iodo-2′-deoxyuridine

Zusammenfassung Nachweis der leukämogenen Wirkung von 5-Azacytidin und 5-Jod-2-Desoxuridin beim Stamm der AKR-Mäuse. Es wird angenommen, dass es sich hierbei um eine Virusinduktion handelt.     

The effects of antineoplastic agents of the respiratory activity of murine lymphocytic leukemia L1210

Résumé On a évalué des effets de plusieurs agents antinéoplastiques sur la consommation d'oxygène des lymphocytes leucémiques L1210 de la souris et on les a mis en corrélation avec l'activité antitumorale de chaque composition. Cette étude indique qu'il n'y a pas de rapport apparent entre ces deux paramètres de l'action des drogues.

---

---

Acknowledgments: The authors wish to express their appreciation to Dr.R. M. Folk of Battelle's Columbus Laboratories for his consultation and advice and toMelanie Crossin for her technical assistance.

---

Supported by Grant No. AM-HE-12084-15 from the National Institutes of Health, Bethesda, Maryland, and Grant No. 7132 from The Ohio State University General Research Support Fund.     

The L-4946 leukemia in the CF1 mouse

Zusammenfassung Mittels Ultrazentrifugation von infektiöser Ascitesflüssigkeit bei 125.000×g und einem gereinigten zellfreien Extrakt konnte ein RNS-Virus als infektiöses Agens und Ursache der Leukämie und eines Lymphosarkoms nachgewiesen werden. Einzelne Mäusestämme erwiesen sich als resistent.

---

---

This study was done at the Department of Biochemistry, University of Chicago, Chicago (Illinois, USA).

---

Supported by research grants No. A1238 and No. GM 424 from the U.S. Public Health Service.     

Antiproliferative effects of 4',5'-unsaturated adenine nucleosides on leukemia L1210 cells in vitro

Several 4',5'-unsaturated adenine nucleosides were shown to have antiproliferative activity against L1210 leukemia cells in vitro. The active nucleosides were cytotoxic to the L1210 cells as demonstrated by Trypan Blue uptake. The cytotoxicity was not induced by alterations in the ribonucleoside and deoxyribonucleoside triphosphate levels of the L1210 cells.     

Effect of 5-azacytidine on developing mouse embryo

Zusammenfassung 5-Azacytidin wurde trächtigen Mäusen am 4.–6. und 11.–13. Tag verabreicht. Bei der ersten Gruppe zeigte sich eine völlige Resorption der Feutusse, während in der zweiten Gruppe hauptsächlich die Leber und das Nervengewebe geschädigt waren. In der ependymalen Zone werden die in Teilung befindlichen Zellen in der Metaphase gestoppt und unterliegen einer pyknotischen Degeneration.     

Growth inhibitory effects of adenosine 3′,5′-monophosphate on mouse leukemia L-5178-Y-R cells in culture

Résumé La croissance des cellules leucémie murine L-5178-Y-R gène le cycle AMP dans les cultures. Le taux des syntèses décroit d'une manière significative après 4 et 6 h pour le RNA et après 8 h pour la protéine. Le taux de la syntèse de DNA décroit quelque peu entre 1 et 6 h et d'une façon accentuée après 8 h.     

Effect of methotrexate on glycolysis of leukemia L 1210 cells cultivated in vitro]

Methotrexate, at non lethal doses, alters the glycolysis of leukaemic L 1210 cells. Glucose uptake and lactate excretion are increased but lactate/glucose ratio remains constant. The activity of glycolytic enzymes in the cytoplasm is increased and the level of intracellular ATP is greatly enhanced.     

Studies on the murine L-4946 ascitic leukemia in Swiss albino mice

Zusammenfassung Virologische Untersuchungen einer Mäuseleukämie, die sich durch eine «subzelluläre onkogene Komponente» übertragen lässt.

---

---

This work was supported in part by the Developmental Physiology Training Grant No. NIH T1-HD0054; NIH Training Grant No. 1 T01 AMO-5680-01 DM; General Research Support Grant No. 623; and aided by a grant from the National Foundation — March of Dimes. This research was conducted in facilities accredited by the American Association for Accreditation of Laboratory Animal Care.     

Partial reversal of toxic effects of 5-azacytidine by 6-azauridine in normal mice

Zusammenfassung Die partielle Blockierung der toxischen Effekte von 5-Azacytidin in normalen Mäusen mit Uridin, Cytidin und 6-Azauridin wird beschrieben. Die Hemmung der toxischen Effekte eines Antimetaboliten durch einen anderen kann für die Krebschemotherapie von Bedeutung sein.     

Depression of experimental gastric ulcers and acute pancreatitis in rats treated by 5-azacytosine, 5-azacytidine and their N-methyl derivatives

5-Azacytosine, 1-methyl-5-azacytosine and 5-azacytidine administered to rats with a ligated pylorus block gastric secretion, gastric acidity, the extent of hemorrhage and the number and size of gastric defects. The same drugs also depress the development of experimental acute pancreatitis mediated in rats by interstitial administration of 7.5% natrium cholate into the pancreas in vivo. The drugs affected the amount of abdominal fluid and 6 h after the treatment the pathological changes were significantly decreased.     

Pycnotic degeneration of ventricular cells in embryonic brain following transplacental exposure to 5-azacytidine

Zusammenfassung Das Cytostaticum 5-Azacytidin verursacht 8 bis 12 h nach Applikation Kernpyknosen in den ventrikelnahen Zellschichten des Gehirns bei Mäuseembryonen, ist aber ohne Wirkung auf die Replikation und die Auswanderung der Zellen.     

Depression of experimental gastric ulcers and acute pancreatitis in rats treated by 5-azacytosine, 5-azacytidine and their N-methyl derivatives

Summary 5-Azacytosine, 1-methyl-5-azacytosine and 5-azacytidine administered to rats with a ligated pylorus block gastric secretion, gastric acidity, the extent of hemorrhage and the number and size of gastric defects. The same drugs also depress the development of experimental acute pancreatitis mediated in rats by interstitial administration of 7.5% natrium cholate into the pancreas in vivo. The drugs affected the amount of abdominal fluid and 6 h after the treatment the pathological changes were significantly decreased.     

The effect of propylthiouracil on glutathione S-transferase activity of rat spleen in vitro and in vivo

Propylthiouracil (PTU) inhibited glutathione (GSH) S-transferase (EC 2.5.1.18) activity of rat spleens in a concentration dependent manner in vitro. PTU (1.5 mmoles/kg) treatment of rats for 1 or 2 weeks caused a decrease in leukocyte number and spleen weight. Nevertheless, GSH S-transferase activity was not affected by the same treatment.     

The effect of cyclic cytidine 3',5'-monophosphate (cCMP) on the in vitro development, hatching and attachment of the mouse blastocyst

The in vitro development and attachment of hatched mouse blastocysts on the untreated substratum was enhanced by 10 microM dibutyryl cCMP (dbcCMP). The result suggests that cCMP has an effect on embryonic development and on the blastocyst attachment process.     

The presence of an endogenous peroxidase activity in hairy cell leukemia cells]

Mononuclear cells from hairy cell leukemia have been studied in three cases by ultrastructural immunocytochemistry. Cells have fairly detectable surface immunoglobulins, without monoclonal distribution however. In addition these cells have a peroxidatic activity which is revealed in the perinuclear space and strands of endoplasmic reticulum.     

Potentiation of the cancerostatic effect of 6-azauridine and 6-azacytidine with 5-bis-(2-chloroethyl)aminomethyluracil

Zusammenfassung Es wird gezeigt, dass die cancerostatische Wirkung von 6-Azauracil und 6-Azacytidin bei Mäusen durch 5-Bis-(2-chloroethyl)aminomethyluracil signifikant potenziert wird.     

Activity of cytidine triphosphate synthetase in normal and neoplastic tissues

Résumé L'activité de la synthétase CTP dans différents tissus normaux et cancéreux a été étudiée. Les résultats indiquent qu'elle est élevée dans les tissus en prolifération.