共查询到20条相似文献,搜索用时 31 毫秒
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Yamaji M Seki Y Kurimoto K Yabuta Y Yuasa M Shigeta M Yamanaka K Ohinata Y Saitou M 《Nature genetics》2008,40(8):1016-1022
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Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells 总被引:1,自引:0,他引:1
Loewer S Cabili MN Guttman M Loh YH Thomas K Park IH Garber M Curran M Onder T Agarwal S Manos PD Datta S Lander ES Schlaeger TM Daley GQ Rinn JL 《Nature genetics》2010,42(12):1113-1117
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The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells 总被引:45,自引:0,他引:45
Loh YH Wu Q Chew JL Vega VB Zhang W Chen X Bourque G George J Leong B Liu J Wong KY Sung KW Lee CW Zhao XD Chiu KP Lipovich L Kuznetsov VA Robson P Stanton LW Wei CL Ruan Y Lim B Ng HH 《Nature genetics》2006,38(4):431-440
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A genome-wide survey of RAS transformation targets 总被引:28,自引:0,他引:28
Zuber J Tchernitsa OI Hinzmann B Schmitz AC Grips M Hellriegel M Sers C Rosenthal A Schäfer R 《Nature genetics》2000,24(2):144-152
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Chromatin immunoprecipitation (ChIP) defines the genomic distribution of proteins and their modifications but is limited by the cell numbers required (ideally >10(7)). Here we describe a protocol that uses carrier chromatin and PCR, 'carrier' ChIP (CChIP), to permit analysis of as few as 100 cells. We assayed histone modifications at key regulator genes (such as Nanog, Pou5f1 (also known as Oct4) and Cdx2) by CChIP in mouse embryonic stem (ES) cells and in inner cell mass (ICM) and trophectoderm of cultured blastocysts. Activating and silencing modifications (H4 acetylation and H3K9 methylation) mark active and silent promoters as predicted, and we find close correlation between values derived from CChIP (1,000 ES cells) and conventional ChIP (5 x 10(7) ES cells). Studies on genes silenced in both ICM and ES cells (Cdx2, Cfc1, Hhex and Nkx2-2, also known as Nkx) show that the intensity of silencing marks is relatively diminished in ES cells, indicating a possible relaxation of some components of silencing on adaptation to culture. 相似文献
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DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal 总被引:1,自引:0,他引:1
The molecular controls that govern the differentiation of embryonic stem (ES) cells remain poorly understood. DGCR8 is an RNA-binding protein that assists the RNase III enzyme Drosha in the processing of microRNAs (miRNAs), a subclass of small RNAs. Here we study the role of miRNAs in ES cell differentiation by generating a Dgcr8 knockout model. Analysis of mouse knockout ES cells shows that DGCR8 is essential for biogenesis of miRNAs. On the induction of differentiation, DGCR8-deficient ES cells do not fully downregulate pluripotency markers and retain the ability to produce ES cell colonies; however, they do express some markers of differentiation. This phenotype differs from that reported for Dicer1 knockout cells, suggesting that Dicer has miRNA-independent roles in ES cell function. Our findings indicate that miRNAs function in the silencing of ES cell self-renewal that normally occurs with the induction of differentiation. 相似文献
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Sox9 is required for cartilage formation. 总被引:32,自引:0,他引:32
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Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells 总被引:1,自引:0,他引:1
Stadtfeld M Apostolou E Ferrari F Choi J Walsh RM Chen T Ooi SS Kim SY Bestor TH Shioda T Park PJ Hochedlinger K 《Nature genetics》2012,44(4):398-405, S1-2