盐酸米诺环素软膏用于慢性根尖周炎的疗效探究

探究盐酸米诺环素软膏用于慢性根尖周炎的疗效。选取2017年2月～2019年3月医院收治的慢性根尖周炎患者95例,按照入院顺序分为2组,均采用根管治疗,观察组采用盐酸米诺环素软膏消毒根管,对照组采用氢氧化钙消毒根管,对比2组临床疗效、疼痛程度、龈沟液中炎性因子变化。观察组治疗总有效率91.67%高于对照组85.11%,但2组差异无统计学意义(P>0.05);观察组Ⅰ级明显高于对照组,Ⅲ级明显低于对照组,2组疼痛程度差异有统计学意义(P<0.05);治疗后,2组患者龈沟液中炎性因子IL-1β、IL-6、TNF-α水平均显著下降,且观察组明显低于对照组,差异有统计学意义(P<0.05)。盐酸米诺环素软膏用于慢性根尖周炎根管治疗消毒中可提升临床疗效,抑制炎症因子表达,减轻炎症反应与疼痛。     

宫颈病变组织中抗增殖蛋白表达与HR-HPV相关性研究

为探讨抗增殖蛋白(Prohibitin,PHB)在宫颈病变组织中的表达与高危型HPV(HR-HPV)的关系。方法:选取采集2014年1月-2015年12月在我院经病理诊断为宫颈病变的132例的患者宫颈组织,分为慢性宫颈炎39例,宫颈上皮内瘤变(CIN)Ⅰ级23例,CINⅡ级-Ⅲ级28例,宫颈鳞癌42例。采用免疫组织化学检测方法检测组织中PHB的表达,杂交捕获二代(HC2)法检测患者宫颈分泌物中HR-HPV-DNA的载量。结果显示:PHB在不同宫颈病变组织中有不同程度的阳性表达,PHB的强阳性率随病变程度的增加逐渐升高,慢性宫颈炎组、CINⅠ级组、CINⅡ-Ⅲ级组、宫颈鳞癌组中PHB的强阳性率分别为35.9%、47.8%、57.1%、71.4%,差异有统计学意义(P0.01)。PHB阳性表达、HR-HPV感染载量均与宫颈病变程度呈正相关,差异有统计学意义(P0.01)。PHB与HR-HPV之间无相关关系,差异无统计学意义(P0.05)。PHB在宫颈鳞癌组的表达与年龄、分化程度、临床分期不相关,差异无统计学意义(P0.05)。由此可知,PHB的过表达及HR-HPV的感染可能从不同途径促进了宫颈癌的发生发展,PHB可能成为宫颈癌潜在的诊断标记物及治疗靶点。     

尿激酶联合阿魏酸钠治疗Lee分级≥III级IgA肾病的疗效观察

观察尿激酶联合阿魏酸钠治疗Lee分级≥Ⅲ级的IgA肾病的临床疗效。入选的46例患者均经肾活检证实可确诊为IgA肾病(IgAN) , Lee分级≥Ⅲ级。将患者随机分为两组, A组: 单用阿魏酸钠; B组: 尿激酶(UK) 联合阿魏酸钠。结果表明, 两组患者治疗前后24 h尿蛋白、肌酐清除率等各项指标均得到不同程度改善, 而且尿激酶联合阿魏酸钠组的疗效更为明显。尿激酶联合阿魏酸钠治疗Lee分级≥III级的IgAN疗效优于单用阿魏酸钠, 并可改善患者的纤溶能力, 为一种安全有效的治疗方案。     

10种大鼠的中医体质学初步研究

实验动物的中医体质学研究是中医实验动物科学的重要一环。本实验对北京市目前 1 0种常用的实验大鼠 :WistarⅠ级、WistarⅡ级、WistarⅢ级、SDⅠ级、SDⅡ级、SDⅢ级、CD(SD)IGSⅢ级 (小体重 )、CD(SD)IGSⅢ级 (大体重 )、SHRⅠ级、SHR/spⅠ级通过检测外观、舌象、下丘脑—垂体—甲状腺轴功能 ,运用中医学理论进行体质初步分析     

CT 和 MRI在胆管细胞癌中的诊断价值对比分析

目的探讨64层螺旋CT扫描与MRI扫描在胆管细胞癌中的诊断价值.方法回顾性分析34例胆管细胞癌患者的临床资料,均行64层螺旋CT平扫、动态增强扫描与MRI平扫及动态增强扫描,分析两种检测手段在胆管细胞癌中的诊断效果.结果 34例胆管细胞癌患者肿块型发生率明显高于腔内生长型、浸润狭窄型、不明确型,64层螺旋CT扫描的图像质量(Ⅰ级29例、Ⅱ级5例、Ⅲ级0例)明显好于MRI扫描(Ⅰ级16例、Ⅱ级14例、Ⅲ级4例),差异有统计学意义(P<0.05).形状不规则肿块型发生率高于圆形与类圆形肿块型发生率,结节状腔内生长型发生率高于菜花状腔内生长型发生率,片状浸润狭窄型发生率高于星芒状浸润狭窄型发生率,64层螺旋CT扫描的定位准确率和定性准确率均高于MRI扫描,差异均无统计学意义(P>0.05).结论 64层螺旋CT扫描与MRI扫描均是胆管细胞癌的有效诊断方法,64层螺旋CT扫描的图像更为清晰,准确性更高.     

血清Ig类、BNP和D-二聚体检测与急性心肌梗死(AMI)的相关性研究

目的探讨急性心肌梗死(AMI)患者血清中IgA,IgG,IgM,BNP和D-二聚体(D-D)浓度的变化与AMI的关系.方法利用免疫比浊法和荧光免疫法对60例AMI患者血清中IgA,IgG,IgM,BNP和D-D浓度进行测定,并与30例正常对照组比较.结果AMI患者血清中的IgA,IgG,IgM,BNP和D-D浓度明显高于正常对照组,与疾病严重程度呈正相关.结论监测AMI患者血清IgA,IgG,IgM,BNP和D-D浓度的变化,对AMI严重程度评估和疗效观察有重要的临床意义.     

人鼻粘膜中IgG、IgE抗体生成细胞的研究(摘要)

作者采用免疫荧光抗体标记技术(直接法)对6例正常人,28例变态反应性鼻炎患者及11例经特异性脱敏治疗后获得较好效果者(共45例)的鼻粘膜中IgG、IgE抗体生成细胞进行了研究,获得五个结果:1、正常人鼻粘膜中具有IgG抗体生成细胞;2、变态反应性鼻炎患者鼻粘膜中IgG抗体生成细胞与正常人无显著差异(P<0.05);3、经特异性脱敏治疗三个月后症状明显改善者鼻粘膜中IgG抗体生成细胞明显高于发作期(P<0.01)。由此推断特异性脱敏可以通过增加局部抗体生成细胞的数量来增加局部IgG封闭抗体的浓度,这     

MR增强检查对幕上星形胶质细胞瘤分级的价值

：探讨磁共振增强检查对幕上星形胶质细胞瘤分级的价值及术后肿瘤复发的征象。方法：选择有完整资料并经手术病理证实的２７例进行回顾性研究,观察其ＭＲ平扫及增强表现,并与病理进行对照。结果：平扫均显示病变为长Ｔ１长Ｔ２异常信号,但信号强度不均、边界不清、病灶附近水肿较多,次为病灶内坏死液化。增强扫描中,２０例有增强表现,２例术后肿瘤复发者可见环状或环壁节状增强。ＭＲＩ分级与病理分级对比：病理诊为Ⅰ～Ⅱ级８例,ＭＲＩ诊Ⅰ级２例,Ⅱ级８例;病理诊Ⅱ级１０例,ＭＲＩ诊Ⅰ级２例,Ⅱ级８例;病理诊Ⅱ～Ⅲ级４例,ＭＲ诊Ⅱ级３例,Ⅲ级１例;病理诊Ⅳ级１例,ＭＲ诊Ⅲ～Ⅳ级。结论：ＭＲ增强检查对幕上星形胶质细胞瘤的分级是有价值的     

元宝枫叶黄酮抑制脂多糖诱导的小胶质细胞激活的作用

 探讨枫叶黄酮对脂多糖(Lipopolysaccharide,LPS)诱导的小胶质细胞株BV-2细胞炎性因子释放的抑制作用.用LPS刺激BV-2细胞构建炎症模型,采用免疫荧光双标和RT-PCR方法检测不同浓度枫叶黄酮(5,10,15μmol/L)对细胞炎性蛋白酶诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和环氧合酶-2 (cyclooxygenase-2,COX-2)、细胞炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-1β (interleukin-1β,IL-1β)、炎性信号蛋白核因子-κB(nuclear factor-κB,NF-κB)蛋白与mRNA的表达变化.结果发现:不同浓度的枫叶黄酮在翻译水平和转录水平上明显抑制了LPS诱导的细胞炎性蛋白酶iNOS和COX-2,细胞炎性因子TNF-α和IL-1β与炎性信号蛋白NF-κB的上调.上述结果表明枫叶黄酮可通过调控LPS诱导的小胶质细胞株BV-2细胞炎性因子释放从而抑制小胶质细胞激活,发挥抗神经炎症的作用.     

葱蜜酒剂对健康兔心功能的影响

本试验用健康日本大耳白兔45只,分为葱蜜酒(Ⅰ)、葱酒(Ⅱ)、蜜酒(Ⅲ)及白酒(Ⅳ)四组。酒剂用冷渍法,用药后临床症状Ⅰ及Ⅲ组腹胀明显。心电图Ⅱ导联波形比较恒定、明显。Ⅰ组用药后P波呈明显上升趋势,T波则明显下降。Ⅱ、Ⅲ、Ⅳ组T波和P波的变化不如Ⅰ组明显。病理组织学变化:Ⅰ组心肌炎最明显(有充血、出血、颗粒变性、心肌纤维断裂,炎性浸润等),Ⅱ组个别家兔有明显心肌灸,炎性细胞浸润较轻,Ⅲ组病变轻微,Ⅳ组病变不明显。     

太行山猕猴消化道肥大细胞分布的研究

太行山猕猴捕自河南省济源市，其消化道组织标本固定于等渗甲醛——醋酸混合液内，用长时间甲苯胺蓝染色法显示肥大细胞．研究结果表明：消化道各段均有肥大细胞存在，且主要分布于粘膜层的固有层，胃的粘膜层中肥大细胞分布密度最大，结肠次之，食管最少．     

ATP drives lamina propria T(H)17 cell differentiation

Interleukin (IL)-17-producing CD4(+) T lymphocytes (T(H)17 cells) constitute a subset of T-helper cells involved in host defence and several immune disorders. An intriguing feature of T(H)17 cells is their selective and constitutive presence in the intestinal lamina propria. Here we show that adenosine 5'-triphosphate (ATP) that can be derived from commensal bacteria activates a unique subset of lamina propria cells, CD70(high)CD11c(low) cells, leading to the differentiation of T(H)17 cells. Germ-free mice exhibit much lower concentrations of luminal ATP, accompanied by fewer lamina propria T(H)17 cells, compared to specific-pathogen-free mice. Systemic or rectal administration of ATP into these germ-free mice results in a marked increase in the number of lamina propria T(H)17 cells. A CD70(high)CD11c(low) subset of the lamina propria cells expresses T(H)17-prone molecules, such as IL-6, IL-23p19 and transforming-growth-factor-beta-activating integrin-alphaV and -beta8, in response to ATP stimulation, and preferentially induces T(H)17 differentiation of co-cultured naive CD4(+) T cells. The critical role of ATP is further underscored by the observation that administration of ATP exacerbates a T-cell-mediated colitis model with enhanced T(H)17 differentiation. These observations highlight the importance of commensal bacteria and ATP for T(H)17 differentiation in health and disease, and offer an explanation of why T(H)17 cells specifically present in the intestinal lamina propria.     

Goblet cells deliver luminal antigen to CD103+ dendritic cells in the small intestine

The intestinal immune system is exposed to a mixture of foreign antigens from diet, commensal flora and potential pathogens. Understanding how pathogen-specific immunity is elicited while avoiding inappropriate responses to the background of innocuous antigens is essential for understanding and treating intestinal infections and inflammatory diseases. The ingestion of protein antigen can induce oral tolerance, which is mediated in part by a subset of intestinal dendritic cells (DCs) that promote the development of regulatory T cells. The lamina propria (LP) underlies the expansive single-cell absorptive villous epithelium and contains a large population of DCs (CD11c(+) CD11b(+) MHCII(+) cells) comprised of two predominant subsets: CD103(+) CX(3)CR1(-) DCs, which promote IgA production, imprint gut homing on lymphocytes and induce the development of regulatory T cells, and CD103(-) CX(3)CR1(+) DCs (with features of macrophages), which promote tumour necrosis factor-α (TNF-α) production, colitis, and the development of T(H)17 T cells. However, the mechanisms by which different intestinal LP-DC subsets capture luminal antigens in vivo remains largely unexplored. Using a minimally disruptive in vivo imaging approach we show that in the steady state, small intestine goblet cells (GCs) function as passages delivering low molecular weight soluble antigens from the intestinal lumen to underlying CD103(+) LP-DCs. The preferential delivery of antigens to DCs with tolerogenic properties implies a key role for this GC function in intestinal immune homeostasis.     

肝炎患者红细胞免疫功能及与体液免疫功能的关系

检测１２１例各型肝炎患者红细胞免疫功能，以及大部分患者的体液免疫功能指标，并探讨两者间的相关性。结果显示：（１）各型肝炎患者及慢性乙肝病毒携带者ＲＢＣＣ３ｂＲＲ均明显低于正常人。急性肝炎急性期和中－重度慢性肝炎患者ＲＢＣ－ＩＣＲ均明显高于正常人。中－重度慢性肝炎患者ＲＢＣＣ３ｂＲＲ明显低于轻度患者，前者ＲＢＣ－ＩＣＲ明显高于后者；急性肝炎恢复期患者ＲＢＣＣ３ｂＲＲ明显高于急性期患者，前者的ＲＢＣ－ＩＣＲ却明显低于后者；（２）急性和慢性肝炎患者血清ＣＩＣ水平均明显升高，急性肝炎恢复期患者血清ＣＩＣ水平较急性期降低。慢性肝炎患者血清ＩｇＭ、ＩｇＧ、ＩｇＡ水平均有不同程度的升高。中－重度慢性肝炎患者血清Ｃ３、Ｃ４水平均明显下降；（３）急性肝炎急性期和恢复期患者ＲＢＣＣ３ｂＲＲ与血清ＣＩＣ水平均呈负相关，而他们的ＲＢＣ－ＩＣＲ与之呈正相关。中－重度慢性肝炎患者ＲＢＣＣ３ｂＲＲ与血清ＣＩＣ水平呈负相关，与血清Ｃ３、Ｃ４水平呈正相关。相反，其ＲＢＣ－ＩＣＲ与血清ＣＩＣ水平呈正相关，与Ｃ３、Ｃ４水平呈负相关。故临床上动态观察肝炎患者的红细胞免疫功能变化，有助于评估病情转归和病变程度。     

慢性热应激对猪肠道CD3^＋T细胞表达的影响

以猪肠道CD3＋T细胞为研究对象,旨在观察慢性热应激对猪肠道黏膜免疫功能的影响。12头小型猪随机平均分成热应激组和常温对照组两组。人工气候室内,热应激组模拟夏季炎热气候,气温从26～39℃24h循环变温,在39℃时维持4h;常温对照组在24℃下饲养。试验持续10d,热应激10d后39℃高温持续期结束剖杀试验猪,取其十二指肠中段、空肠及回肠中段进行固定、石蜡切片、免疫组织化学染色,观察十二指肠、空肠及回肠中CD3＋T细胞数量的变化。结果显示：实验猪十二指肠黏膜上皮层、固有层及十二指肠肠腺均呈CD3强阳性,与对照组相比,CD3阳性面积系数显著上升（P〈0.05）;空肠和回肠黏膜上皮层的CD3阳性细胞数量也显著增加（P〈0.05）,而固有层中的CD3阳性细胞数量则无明显变化,增加的幅度小于十二指肠。慢性热应激中实验猪肠道结构严重受损,消化吸收功能严重下降,而肠道CD3＋T细胞的增多,可能是机体肠道黏膜免疫功能的一种代偿性增强反应。     

Insights into IgA-mediated immune responses from the crystal structures of human FcalphaRI and its complex with IgA1-Fc

Immunoglobulin-alpha (IgA)-bound antigens induce immune effector responses by activating the IgA-specific receptor FcalphaRI (CD89) on immune cells. Here we present crystal structures of human FcalphaRI alone and in a complex with the Fc region of IgA1 (Fcalpha). FcalphaRI has two immunoglobulin-like domains that are oriented at approximately right angles to each other. Fcalpha resembles the Fcs of immunoglobulins IgG and IgE, but has differently located interchain disulphide bonds and external rather than interdomain N-linked carbohydrates. Unlike 1:1 FcgammaRIII:IgG and Fc epsilon RI:IgE complexes, two FcalphaRI molecules bind each Fcalpha dimer, one at each Calpha2-Calpha3 junction. The FcalphaRI-binding site on IgA1 overlaps the reported polymeric immunoglobulin receptor (pIgR)-binding site, which might explain why secretory IgA cannot initiate phagocytosis or bind to FcalphaRI-expressing cells in the absence of an integrin co-receptor.     

Peak SIV replication in resting memory CD4+ T cells depletes gut lamina propria CD4+ T cells

In early simian immunodeficiency virus (SIV) and human immunodeficiency virus-1 (HIV-1) infections, gut-associated lymphatic tissue (GALT), the largest component of the lymphoid organ system, is a principal site of both virus production and depletion of primarily lamina propria memory CD4+ T cells; that is, CD4-expressing T cells that previously encountered antigens and microbes and homed to the lamina propria of GALT. Here, we show that peak virus production in gut tissues of SIV-infected rhesus macaques coincides with peak numbers of infected memory CD4+ T cells. Surprisingly, most of the initially infected memory cells were not, as expected, activated but were instead immunophenotypically 'resting' cells that, unlike truly resting cells, but like the first cells mainly infected at other mucosal sites and peripheral lymph nodes, are capable of supporting virus production. In addition to inducing immune activation and thereby providing activated CD4+ T-cell targets to sustain infection, virus production also triggered an immunopathologically limiting Fas-Fas-ligand-mediated apoptotic pathway in lamina propria CD4+ T cells, resulting in their preferential ablation. Thus, SIV exploits a large, resident population of resting memory CD4+ T cells in GALT to produce peak levels of virus that directly (through lytic infection) and indirectly (through apoptosis of infected and uninfected cells) deplete CD4+ T cells in the effector arm of GALT. The scale of this CD4+ T-cell depletion has adverse effects on the immune system of the host, underscoring the importance of developing countermeasures to SIV that are effective before infection of GALT.     

玉屏风散对于单板U型场地项目运动员免疫能力影响的临床研究

研究论证玉屏风散对于单板U型场地项目运动员大强度训练免疫机能的影响.方法：以哈尔滨体育学院单板U型场地滑雪运动员为研究对象,运用临床试验法,以玉屏风散为干预手段,测试了NK细胞毒活性、IgG、IgM、IgA、IL-2等指标,研究论证玉屏风散对于运动员大强度训练免疫功能的调节作用,并对其作用机制进行初步研究探讨.结果：试验组NK细胞调节具备一定上升趋势;试验组试验后与试验前自身IgG、IgM、IL-2比较,显著上升,IgA具备一定上升趋势.对照组试验后免疫机能出现一定下降趋势.结论：运动员在低温大强度训练后可能会出现一定程度的免疫力降低,玉屏风散对于低温大强度训练的单板U型场地项目运动员免疫力具有一定增强作用.