Ovarian HCG-binding in hemicastrated immature female rats

Summary Hemigonadectomy in 9-day-old female rats results in a drastic increase in the weight and the number of HCG-binding sites of the remaining ovary during further development. However, on a per mg basis of ovarian weight, the number of HCG-receptors is identical in hemicastrated and control rats.     

Effects of female sex hormones on polyamine-oxidizing enzyme activities and polyamine concentrations in immature rat uterus and liver

17-estradiol (E₂) and progesterone (P) treatment of immature female rats (10 g/100 g body weight) respectively resulted in 1.38-fold (p<0.02) and 1.42-fold (p<0.02) increase in the uterine polyamine oxidase activity, and 2.45-fold (p<0.001) and 1.43-fold (p<0.02) increase in the uterine diamine oxidase activity, as compared to the controls. E₂ caused a 5-fold (p<0.05) and a 1.36-fold (p<0.05) increase in putrescine and spermidine concentration respectively in rat uterus. Increases of 1.7-fold (p<0.02) and 1.6-fold (p<0.05) in putrescine and spermine concentration were determined in the P-treated uterus, as compared to the controls. The spermidine/spermine ratio, which is regarded as an index of growth rate, was higher in the E₂-treated uterus and lower in the P-treated uterus than in the control uterus. No statistically significant hormonal effects were estimated in the immature liver. The data reported suggest the possibility of an involvement of polyamine-oxidizing enzymes in the modulation of polyamine concentrations in rat uterus by the female sex hormones.     

Distribution of noradrenaline in the genital organs of the female rat with a remark on dopamine in the cervix and vagina

Zusammenfassung Durch Bestimmung des organeigenen Noradrenalingehalts wird die adrenergische Innervation der weiblichen Geschlechtsorgane der Ratte untersucht. Die Hauptmenge des Noradrenalins (0,3µg/g) wird im Tuba uterina gefunden, der vierfache Betrag von Uterusinhalt. Daneben wurde Dopamin in Cervix und Vagina nachgewiesen.

---

---

This work has been supported by Stiftelsen Therese och Johan Anderssons Minne and by the Swedish Medical Research Council, project No. B68-14X-2323-01.     

Effect of testosterone propionate on the urogenital organs of immature crocodileCrocodylus palustris lesson

Zusammenfassung Nicht geschlechtsreifen, zweijährigen Krokodilen aus gleicher Brut wurden je 25 mg (Gesamtdosis) Testosteronpropionat verabreicht. Sektionsbefund 7 Tage nach der letzten Injektion: Spermatogenese und entwickelte Spermien, Rückbildung des interstitiellen Gewebes. Penis und Klitoris hypertrophisch. Nierenvergrösserung, Ovarien und Müllersche Gänge unverändert. Kontrollen ohne Spermatogenese, jedoch mit Vermehrung der Leydigschen Zellen.

---

---

Investigation made possible from a grant from the Ford Foundation, New Delhi.     

The involvement of serotonin in induced ovulation in the immature rat

Summary In pregnant mare's serum gonadotropin (PMS) treated immature rats the cortex, cerebellum, caudate nucleus and hypothalamus were isolated and analyzed for their serotonin (5-HT) content at 6-h intervals for 72 h. Results showed a general trend of significant variation occurring in days 1 and 3 after PMS injection with no major variations observed on the second day. The results obtained suggest a possible involvement of 5-HT in the control of ovulation.     

Effect of an LH-RH antagonist on reproductive status of immature female rats

Summary Administration of the LH-RH antagonist, D-Phe²-D-Ala⁶-LH-RH (Wy-18, 185) to immature female rats from days 25–35 of age was without significant effect on day of vaginal opening (puberal onset), weights of the ovaries, uteri and anterior pituitary, and on ovarian histology on autopsy day 39.The technical expertise of J. Tracy is gratefully acknowleged.     

Effect of p-coumaric acid on immature estrogen treated and cyclic female mice

Oral administration of p-coumaric acid to estrogen-primed immature female mice exerts neither estrogenic nor anti-estrogenic activity, but when it is administered to cyclic female mice in single dose at proestrus or in repeated doses, induces alteration in estrus cycle, ovarian and uterine weight and structure.     

Effect of p-coumaric acid on immature estrogen treated and cyclic female mice

Summary Oral administration of p-coumaric acid to estrogen-primed immature female mice exerts neither estrogenic nor anti-estrogenic activity, but when it is administered to cyclic female mice in single dose at proestrus or in repeated doses, induces alteration in estrus cycle, ovarian and uterine weight and structure.The authors wish to thank Dr S. C. Pakrashi and his associates of Medicinal Chemistry Department for isolating the compound. Thanks are also due for granting Senior Research Fellowship to P.P. and Junior Research Fellowship to S.N.K. respectively.     

Distribution of 4-iodine-antipyrine (I131) in the rat

Resumen La determinación de agua total y de agua tisular se efectuo por medio de la dilución de 4-yodo-antipirina (I¹³¹) en la rata. Los resultados obtenidos muestran que la distribución regional del indicador no es similar a la del agua, aunque el volumen de agua total encontrado se aproxima a los valores medios normales. Esta distribución del indicador en los tejidos no hace aconsejable la utilización de este indicador para la medición del volumen de agua total o regional.     

The involvement of serotonin in induced ovulation in the immature rat.

In pregnant mare's serum gonadotropin (PMS) treated immature rats the cortex, cerebellum, caudate nucleus and hypothalamus were isolated and analyzed for their serotonin (5-HT) content at 6-h intervals for 72 h. Results showed a general trend of significant variation occurring in days 1 and 3 after PMS injection with no major variations observed on the second day. The results obtained suggest a possible involvement of 5-HT in the control of ovulation.     

Effect of an LH-RH antagonist on reproductive status of immature female rats.

Administration of the LH-RH antagonist, D-Phe2-D-Ala6-LH-RH (Wy-18, 185) to immature female rats from days 25--35 of age was without significant effect on day of vaginal opening (puberal onset), weights of the ovaries, uteri and anterior pituitary, and on ovarian histology on autopsy day 39.     

Fine structural differentiation of steroid-producing cells in the ovary of immature rat

Zusammenfassung Steroid-produzierende Zellen wurden in der Theca folliculi der geschlechtsunreifen Ratten vom 10. Lebenstag an elektronenmikroskopisch gefunden.     

FSH-releasing effect of clomiphene in the female rat

Zusammenfassung Der Effekt von Clomifendihydrogenzitrat auf den FSH-Release der Ratte wurde untersucht. Die i.v. Injektion von 300 bzw. 600 µg Clomifendihydrogenzitrat/Tier führte bei oophorektomierten, weiblichen Ratten, die mit Östradiol und Progesteron blokkiert worden waren, zu einer signifikanten Erhöhung der FSH-Aktivität im Plasma. Eine Beeinträchtigung des FSH-Nachweises durch noch im Plasma befindliches Clomifendihydrogencitrat konnte ausgeschlossen werden.     

Metallothionein induction by cadmium and zinc in rat secretory organs

Summary Metallothionein (MT) levels were determined in four secretory organs of the rat following administration of zinc (Zn) and cadmium (Cd). The concentrations of MT in the lacrimal, parotid and adrenal glands of untreated rats were in the range of 2.2–4.9 g/g wet weight tissue while in the pancreas it was shown to be 15.2 g/g. Injection of zinc at total doses of 16, 32 and 80 mg/kg resulted in a 1.8-, 3.2- and 5.9-fold increase in lacrimal MT content, respectively, while a 10.2- and 13.1-fold elevation was observed following treatment with 4 and 8 mg/kg of Cd, respectively. Similar findings were found in the adrenal gland. The parotid MT was elevated 5.9 and 17 times following Zn treatment at doses of 16 and 80 mg/kg respectively, whereas 4 mg/kg of Cd increased MT 14.4 times in this gland. Pancreatic MT was elevated by 39- and 40-fold after injection of Zn at doses of 16 and 32 mg/kg respectively, whereas 4 and 8 mg/kg of Cd caused a 9.8- and 17.9-fold induction, respectively. These results may indicate that secretory organs participate in metabolism of heavy metals in the mammalian body.