泥蚶育苗期间单胞藻稳产技术初探

随着泥蚶人工育苗技术的不断发展完善,稳定生产单胞藻饵料以满足幼体生长发育的需要已成为需要突出解决的问题。本文结合生产实际,提出几项措施仅供参考。1种类选择在泥蚶幼体培育阶段,幼体对藻类大小、种类有所选择。D形幼虫开口摄食时,以个体较小为宜,如球等鞭金藻3011（Isochorysisgalbansgalbans）、湛江叉鞭金藻（DicrateriaZhangensisHuVraSp．）、绿帕夫3012（Pavlovariridisgalbana）。至后期,为了使营养全面利于幼体顺利变态附着,以金藻、角毛藻（ChaeocerosSp．）及扁藻（PlatymonasSp．）混合投喂为好。附着后利用扁…     

泥蚶数量性状的相关分析

测量了泥蚶壳长、壳宽、壳高等壳性状和活体重、软体部重等经济性状。分析表明，泥蚶各性状的相关系数均达到极显著水平(P＜0．01)。壳高与壳长、壳宽，壳长与壳宽，体重与软体部重呈线性相关；壳高与体重、软体部重呈幂函数相关。多元回归分析建立的壳长(SL)、壳高(SH)和壳宽(SW)估计体重(WB)和软体重(WF)的回归方程为：WB=-3．08 0．05 SL 0．67 SW-0．22 SH，WF=-1．04 0．04 SL 0．18 SW-0．07 SH。     

泥蚶的性腺发育和生殖周期

根据生殖细胞发育的宏观和组织切片观察以及各期生殖细胞在滤泡中所占的比例，泥蚶的生殖腺发育过程分为增殖期、生长期、成熟期、排放期和休止期五个阶段。按肥满度的变化并结合性腺组织切片确定，泥蚶的性腺发育在青岛海区一年一个周期。繁殖期在７月上旬至８月下旬，繁殖盛期在７月中旬互８月中旬。至少有二次集中排放过程。     

泥蚶生产性育苗中若干培育因子的研究

StudyonSeveralBreedingFactorsintheCommercialBreedingofAreagranosaZhengZhongming（FisheriesDepartmentofNingboUniversity，Ningbo315211）泥姐Tegillarcagranosa（Linnaeus）肉味鲜美，是一种广温广盐性软体双壳经济贝类，广泛分布于印度洋、太平洋沿岸潮间带的中、低潮区。泥燃在我国养殖历史悠久，现为山东、浙江。福建、广东的主要养殖品种。泥蛐人工育苗经过多年努力，目前在全国已普及，尤其在浙江省近几年已形成相当的规模，但在生产过程中，仍出现大起大落的局面，主要问题大多出在附着及附着以前阶段。所以如何稳定和…     

泥蚶体液凝集素性能的初步研究

在泥蚶(Tegillarca granosa Linnaeus)体液中发现有凝集素存在.利用14种天然或经酶处理的动物和人红细胞来测定泥蚶体液凝集素的凝集活力,并且进行糖(200 mmol·L-1)抑制、热稳定性、pH及Ca2 影响试验.结果显示:泥蚶体液凝集素对14种红细胞均有凝集作用.其中对兔红细胞的凝集作用可被乳糖和半乳糖所抑制,凝集活性依赖于Ca2 ,在pH7.0较稳定,温度为40～60℃范围内均具较高活性,经90℃保温10 min后活性完全消失.     

不同种群泥蚶的三种同工酶变异

采用聚丙烯酰胺凝胶电泳方法,对广西自然种群、乐清自然种群、山东自然种群、韩国自然种群和乐清养殖种群泥蚶(Tegillarcagranosa)的过氧化物酶、酯酶及淀粉酶等3种同工酶进行了研究。结果表明,POD同工酶除韩国自然种群外,其它种群的表达一致;AMY同工酶除乐清养殖种群外,其它种群的表达一致。EST酶谱中,广西自然种群与乐清自然种群的酶谱表达一致,而山东自然种群、乐清养殖种群和韩国自然种群的表达存在一定的差异。     

泥蚶人工育苗高产技术的研究

研究泥蚶人工育苗的若干技术。提出一些高产措施：如优选亲蚶；高密度采卵再分池洗卵、孵化，高密度培育面盘幼虫；无“底质”采集跟点幼虫，细砂（含极少量泥质和有机质）底质培育稚蚶，加大投饵量和换水量；严格防病等。在生产中取得一定的效果，平均单产稚蚶（壳长０．９±０．２ｍｍ）３２．９０万粒／ｍ＾２，最高单产５９．４４万粒／ｍ＾２。     

温度对泥蚶胚胎发育、幼体生长发育及变态的影响

用广西防城港海区２～３龄增均体重１０克／只～１２克只的泥蚶作为新贝,对泥蚶胎发育、幼体生长发育及变态与温度的关系进行试验研究;试验温度为１９℃、２１℃、２３℃、２５℃、２７℃、２９℃、３１℃、３３℃。试验用水为沙滤海水,比重１．０１４～１．０２０,ｐＨ值８．０～８．２。结果表明,胚胎发育最适温度为２５℃～３１℃,由此温度带求得的胚胎发育温度系数Ｑ１０为２．０５。在高低极端温度下,胚胎发育畸形而死亡     

江苏种群橄榄蚶同工酶的生化遗传分析

采用聚丙烯酰胺凝胶不连续垂直平板电泳技术对江苏种群橄榄蚶不同个体的苹果酸脱氢酶、苹果酸酶、醇脱氢酶、乳酸脱氢酶、淀粉酶、酯酶、超氧化物歧化酶和细胞色素氧化酶8种同工酶进行分析,得到基本图谱,并对其进行了生化遗传分析．共记录了21个基因座位、38个等位基因,其中有11个基因座位（Me-1、Adh-2、Adh-3、Ldh-1、Ldh-2、Amy-1、Amy-2、Est-2、Sod-2、Cod-1和Cod-2）为多态,多态座位比例（P）为0．5238,该比例接近一般海产无脊椎动物的多态座位比例,表明江苏种群橄榄蚶具有一定的遗传变异能力．     

不同饵料投喂对魁蚶幼体生长的影响

【目的】探讨在魁蚶苗种繁育中如何选择合适的饵料,了解魁蚶幼体生物学特性。【方法】选取金藻、角毛藻、海洋酵母、小球藻等4种不同饵料对魁蚶幼体进行为期13d的喂养,测定其壳长、壳高等生物学指标,计算特定生长率,分析不同饵料投喂对魁蚶幼体生长的影响。【结果】从D形幼虫发育到壳顶期这段时期内,混合饵料金藻+海洋酵母较单一饵料投喂魁蚶幼虫的效果好。不同饵料投喂下魁蚶幼虫特定生长率大小依次为金藻+海洋酵母处理组金藻处理组海洋酵母处理组金藻+角毛藻处理组角毛藻处理组小球藻处理组,各处理组间差异极显著(P0.05)。【结论】在魁蚶幼体培育阶段,最佳饵料是金藻+海洋酵母。在没有混合饵料的前提下,金藻效果最好,适合做幼虫的开口饵料;小球藻效果最差,不合适做单一饵料投喂幼虫。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that