The histochemical demonstration ofβ-d-glucuronidase in the reticuloendothelial system

Zusammenfassung Eine Halogen enthaltende Indolverbindung wurde zum Nachweis der-d-Glucuronidase benutzt. Die Methode eignet sich zur Lokalisierung von reaktiven Kleinzellen im reticuloendothelialen System.     

Electrophysiology of mammalian hypothalamic and interpeduncular neurons in vitro

Summary Electrical activities of the hypothalamic and interpeduncular neurons were studied in vitro in brain slices prepared from the guinea-pig brain stem. Neurons preserved resting membrane potentials comparable to those of neurons in vivo, responded to stimulation of the afferent fibres, and retained stable spontaneous firings for more than several hours.     

Autoradiographic demonstration of estrogen uptake by the armadillo pituitary gland

Summary Adult female armadillos were injected with tritiated estradiol. The pituitary gland was removed and processed for autoradiography. Nuclear uptake and retention of the steroid was found in 28.8% of the cells in the pars distalis and 3.7% of the pituicytes.This study was supported in part by USPHS Research Career Development Award NS 00164 (PJS) and USPHS Grants NS 12933, HD 10914, HD 10202, and RR 07187.     

Electrophysiology of mammalian hypothalamic and interpeduncular neurons in vitro.

Electrical activities of the hypothalamic and interpeduncular neurons were studied in vitro in brain slices prepared from the guinea-pig brain stem. Neurons preserved resting membrane potentials comparable to those of neurons in vivo, responded to stimulation of the afferent fibres, and retained stable spontaneous firings for more than several hours.     

The indigogenic reaction for histochemical demonstration of alkaline and acid phosphatase

Résumé Le principe indigogénique à la détection histochimique des phosphates alcalins et acides a été appliqué. Ces substrates ont l'avantage de permettre une localisation précise de l'enzyme, sans ou avec minime diffusion. C'est aussi une méthode simple et directe pour la mise en évidence de ces enzymes sans passer par une réaction couplée. Un bisindigo très insoluble a lieu apparaît aux points d'activité enzymatique. Le 5-bromo-4-chlorocomposé donne un indigo bleu-vert tandis que le 5-bromo-6-chloro-composé donne un indigo magenta.     

Direct histochemical demonstration of histamine in cutaneous mast cells: Urticaria pigmentosa and keloids

Zusammenfassung Die Haut von Patienten mit Urticaria pigmentosa und Keloiden wurde mit der histochemischeno-Phthaldialdehyd-Technik untersucht. Die Lokalisation von Histamin konnte durch Fluoreszenzmikroskopie festgestellt werden.     

Insulin acts on the hypothalamic glucose-facilitated neurons to induce hyperglycemia and hyperinsulinemia in the rat

Microinjection of insulin (0.04-0.12 IU/microliter) into the anterior hypothalamus or the lateral hypothalamus, but not the ventromedial hypothalamus of the rat brain, caused a dose-dependent rise in blood glucose and in serum insulin. The majority (71.5%) of the glucose-facilitated neurons recorded in the lateral hypothalamic area were excited by intracerebral injection of insulin. The data indicate that insulin acts on the hypothalamic glucose-facilitated neurons to induce hyperglycemia and hyperinsulinemia. It is unknown whether insulin normally reaches the hypothalamic area, or how it might do so.     

Insulin acts on the hypothalamic glucose-facilitated neurons to induce hyperglycemia and hyperinsulinemia in the rat

Microinjection of insulin (0.04–0.12 IU/l) into the anterior hypothalamus or the lateral hypothalamus, but not the vertromedial hypothalamus of the rat brain, caused a dose-dependent rise in blood glucose and in serum insulin. The majority (71.5%) of the glucose-facilitated neurons recorded in the lateral hypothalamic area were excited by intracerebral injection of insulin. The data indicate that insulni acts on the hypothalamic glucose-facilitated neurons to induce hyperglycemia and hyperinsulinemia. It is unknown whether insulin normally reaches the hypothalamic area, or how it might do so.This work was supported by grants from the National Science Council (Taipei, Taiwan, Republic of China).     

Autoradiographic demonstration of uptake and accumulation of3H-6-hydroxydopamine in adrenergic nerves

Zusammenfassung Die Regenbogenhaut der Maus wurde in einer physiologischen Pufferlösung mit radioaktivem 6-Hydroxydopamin (³H-6-OH-DA) inkubiert. Mit Hilfe autoradiographischer und fluoreszenz-histochemischer Untersuchungen konnte gezeigt werden, dass³H-6-OH-DA in die adrenergischen Nervenfasern der Regenbogenhaut aufgenommen und gespeichert wird. Dieser Befund bringt die direkte Erklärung der durch 6-Hydroxydopamin induzierten Degeneration der adrenergen Fasern.     

Increase in the hypothalamic tyrosine hydroxylase activity after lesion of the catecholaminergic neurons of the rat pontine tegmentum]

Hypothalamic tyrosine hydroxylase activity is increased 6 and 18 h after bilateral lesioning of the Locus Coerulues, in the Rat. An adrenocortical hypertrophy was observed previously, under the same experimental conditions. The neurochemical and neuroendocrinological implications of theses results are discussed.     

Electrophysiologic and anatomic demonstration of inhibitory commisural vestibular neurons in the tench (Tinca tinca)]

Horseradish peroxydase was injected intracellularly in some of the medullary neurons which, in Fish, exhibit a passive hyperpolarizing potential after spinal cord stimulation (this indicates that such neurons both electrical and chemical inhibitions of the Mauthner cells). With positive staining, commissural vestibular neurons were identified; their axons cross the midline and they establish connexions with the Mauthner cell and with vestibular neurons of both sides. Thus the existence of a crossed vestibular inhibitory pathway, previously suggested by electrophysiological studies in lower Vertebrates as well as in Mammals, is now correlated with histological evidence.     

A histochemical demonstration of the Na++K+-ATPase activity in the thyroid and the effect of cyclic adenosine monophosphate (c-AMP)

Summary With a suitable modification of the Farquhar and Palade technique the Na⁺+K⁺-ATPase activity in guineapig thyroid is demonstrated. The addition of c-AMP (5×10^–6 M or 1.5×10^–5 M) to the incubation media produced an apparent intensification of the Na⁺+K⁺-ATPase activity in the thyroid.This work was supported by a grant from ZMNU of Serbia.     

Interaction between the effects of centrally administered arecoline and leucocyte pyrogen on the activity of posterior hypothalamic neurons in the rabbit

Summary In experiments with urethane-anesthetized rabbits, the alteration in the activity of posterior hypothalamic neurons resulting from intracerebroventricular injection of leucocyte pyrogen was attentuated by subsequent administration of arecoline. Atropine failed to alter the neuronal response to leucocyte pyrogen but abolished the effect of arecoline. The neuronal response to arecoline was reversed in the absence of leucocyte pyrogen.     

Interaction between the effects of centrally administered arecoline and leucocyte pyrogen on the activity of posterior hypothalamic neurons in the rabbit

In experiments with urethane-anesthetized rabbits, the alteration in the activity of posterior hypothalamic neurons resulting from intracerebroventricular injection of leucocyte pyrogen was attenuated by subsequent administration of arecoline. Atropine failed to alter the neuronal response to leucocyte pyrogen but abolished the effect of arecoline. The neuronal response to arecoline was reversed in the absence of leucocyte pyrogen.     

A histochemical demonstration of the Na+ + K+-ATPase activity in the thyroid and the effect of cyclic adenosine monophosphate (c-AMP).

With a suitable modification of the Farquhar and Palade technique the Na+ + K+-ATPase activity in guinea-pig thyroid is demonstrated. The addition of c-AMP (5 X 10(-6) M or 1.5 X 10(-5) M) to the incubation media produced an apparent intensification of the Na+ + K+ -ATPase activity in the thyroid.     

The uptake of35S by hypothalamic and neurohypophysial proteins following intraventricular injection ofl-cysteine-35S-hydrochloride in rats dehydrated and reserpinized

Summary In rats dehydrated for 48 h and injected intraventricularly withl-cysteine-³⁵S-hydrochloride, the specific activity of TCA-precipitable material, both in the hypothalamus and neurohypophysis, was found to diminish under reserpine treatment.