Determination of adhesive rate constant in normal and neoplastic homogeneic cells

Summary The adhesive rate constant (ARC) of neoplastic SGS-2 cells which have a low contact inhibition, is remarkably higher than that of normal homogeneic fibroblasts. This is in contrast with the mutual adhesion theory which states that the loss of contact inhibition is strictly related to the loss of cell recognition and consequently to the loss of cell adhesion capacity.This research was supported in part by the Italian CNR Rome, grant No. 79.00955.04 and in part by the Swiss National Science Foundation, Berne, grant No. 3.499.079. The skilful technical assistance of Mrs Mirjam Valluchi is gratefully acknowledged.     

Effects of lithium chloride on peripheral acetylcholine release and brain acetylcholine levels in the guinea-pig

Summary Lithium chloride administered acutely or chronically to guinea-pigs had no effect on brain level of acetylcholine or on peripheral release of acetylcholine from longitudinal muscle of the ileum. The results suggest differences between in vitro and in vivo action of lithium.To whom correspondence should be addressed.     

Structure of gap junctions in cultures of normal and neoplastic bladder epithelial cells

Summary Normal rat urinary bladder epithelial cells contain small subunit (PF-1) and large subunit (PF-2) gap junctions, whereas carcinoma cells only contain PF-1 gap junctions. The absence of PF-2 gap junctions, which are composed of larger connexons with slightly larger ionic channels, may contribute to altered metabolic coupling between urinary bladder carcinoma cells.Acknowledgments. The authors wish to thank Ms Denise Wiler and Mr William Leonard for expert technical assistance. This work was supported by funds from the National Cancer Institute, CA-25034, and in part by the Otho SA Sprague Memorial Institute.     

Recognition sites involved in the adhesive processes of neoplastic and normal homogeneic cells

Cross-adhesion experiments between neoplastic and normal homogeneic cells show that trypsin-sensitive recognition sites are involved in cell-cell adhesion of neoplastic cells and that the affinity between heterologous recognition sites is higher then between homologous ones.     

Effects of lithium chloride on peripheral acetylcholine release and brain acetylcholine levels in the guinea-pig.

Lithium chloride administered acutely or chronically to guinea-pigs had no effect on brain level of acetylcholine or on peripheral release of acetylcholine from longitudinal muscle of the ileum. The results suggest differences between in vitro and in vivo action of lithium.     

Histamine release by passive sensitization with murine neoplastic mast cells in vitro

Riassunto Mastociti neoplastici di un clono diploide, coltivati nel topo come tumore-ascite, sono stati impiegati per produrre nel coniglio un antisiero specifico verso celiule dello stesso clono. L'incubazione con l'antisiero produce liberazione di istamina da parte di mastociti dello stesso clono; l'effetto non si osserva se i mastociti sono incubati con sieri ottenuti dagli stessi animali prima della sensibilizzazione. Viene prospettato 1'uso di questo modello sperimentale per sue possibili applicazioni allo studio iella liberazione dell'istamina nella sensibilizzazione passiva dei mastociti.

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The present investigation has been aided by grant NB 00940 from the National Institute of Neurological Diseases and Blindness. The experiments have been performed during one year's tenure of a N.A.T.O. Fellowship at the Department of Pharmacology, Yale University, New Haven, Connecticut.     

Effect of lithium on normal and chronic granulocytic leukemia colony forming cells (CFU-GM)

Summary The effect of lithium on normal and chronic granulocytic leukemia (CGL) granulo-monocytic precursors (CFU-GM) has been studied. Lithium slightly increases normal CFU-GM growth whereas it is without effect or moderately inhibitory for CGL CFU-GM. It is suggested that it is unlikely that lithium therapy enhances the proliferation of a silent leukemic clone.Acknowledgments. The authors wish to thank Prof. F. Gavosto for his suggestions. This work was supported by CNR, PFCCN, grant No.80.0155796.     

Effect of lithium on normal and chronic granulocytic leukemia colony forming cells (CFU-GM)

The effect of lithium on normal and chronic granulocytic leukemia (CGL) granulo-monocytic precursors (CFU-GM) has been studied. Lithium slightly increases normal CFU-GM growth whereas it is without effect or moderately inhibitory for CGL CFU-GM. It is suggested that it is unlikely that lithium therapy enhances the proliferation of a silent leukemic clone.     

Activity of cytidine triphosphate synthetase in normal and neoplastic tissues

Résumé L'activité de la synthétase CTP dans différents tissus normaux et cancéreux a été étudiée. Les résultats indiquent qu'elle est élevée dans les tissus en prolifération.     

Release of plasminogen activator from normal and neoplastic endometrium

Summary In the medium of endometrial carcinoma cultures, anti-urokinase-reacting plasminogen activator was released in contrast to cultures of normal or hyperplastic endometrium.This investigation was supported by grants from Malmö General Hospital, the Council for Tobacco Research, USA and the Swedish Medical Research Council (B79-17X-04523-05B).     

Reexamination of vertical activity in rats treated with lithium chloride

Summary Injection of hypertonic LiCl i.p. has several nonspecific adverse effects in rats. No evidence was obtained forJohnson's hypothesis that the effect of LiCl on rearing is mediated by environmental stimuli.Acknowledgments. The author thanks Aarhus University, The Danish Medical Research Council, andMarianne Jensen for assistance.     

Effects of antigen on in vitro cultures of sensitized peritoneal cells

Résumé On a ajouté des antigènes aux cultures in vitro des cellules mononucléaires obtenues du péritoine des 3 groupes de cobayes, le premier étant normal, le deuxième présentant une sensibilité immédiate et le troisième une hypersensibilité retardée à la tuberculine ou à l'ovalbumine. L'addition de l'antigène spécifique aux cultures des cellules obtenues des cobayes avec hypersensibilité retardée a provoqué la formation de cellules avec 2 ou plusieurs noyaux. Ceci suggère une relation étroite entre l'hypersensibilité retardée et la formation de cellules multinucléaires.     

Release of plasminogen activator from normal and neoplastic endometrium.

In the medium of endometrial carcinoma cultures, anti-urokinase-reacting plasminogen activator was released in contrast to cultures of normal or hyperplastic endometrium.     

Irradiation induced neoplastic and giant cells in earthworms

Zusammenfassung Verschiedenes, durch Röntgenbestrahlung hervorgerufenes Regenwurmneoplasma erweist sich den Vertebraten-Mioblastoma ähnlich. In solchen Tumoren werden zuweilen Riesenkerne gefunden. Bestrahlte Würmer weisen ebenfalls vielkernige Riesenzellen auf.