Intérêt du séquençage dans le diagnostic virologique : application au VIH et au VHC

Sequencing is an important tool for viral typing and is also used to analyse resistant genotypes. However, in current hospital practice, it is only used for HIV and HCV. In effect, it is important to monitor the genetic diversity of these two viruses as this has implications for diagnosis (antibody and viral genome detection), plasma RNA quantification and therapeutic strategy. Several methods exist for typing or genotyping. The standard method is still nucleotide sequencing followed by phylogenetic analysis. In addition, it is only by sequencing several regions of the genome that recombinant viruses can be identified in certain cases. The introduction of systematic sequencing of resistant HIV in medical virology laboratories will favour data accumulation. The available data have already served to establish decision-making algorithms that need to be continually adapted and updated with evolving knowledge and the appearance of new molecules.