HPV16、18型E6/E7融合基因痘苗病毒表达载体的构建

目的:构建一种含人乳头瘤病毒(HPV)16型、18型E6/E7融合基因痘苗病毒表达载体.方法:PCR扩增HPV16型、18型E6、E7基因,克隆到pSC-A载体中,通过定点突变方法,分别构建成含E6/E7融合基因的质粒,即pSC-A.HPV16 E6/E7和pSC-A-HPV18 mE6/E7,并将二者连接成载体pSC-A-HPV16 E6/E7-HPV18mE6/E(pSC-T,HPV16 E6/E7-HPV18mE6/E7:T),最后以痘苗病毒表达载体pJ38为转移载体,构建质粒pJ38-HPV16 E6/E7-HPV18mE6/E7(pJ38-T),对所有重组质粒进行酶切鉴定和测序分析.结果:E6/E7融合基因成功克隆到pJ38上.结论:成功构建表达HPV E6/E7融合基因重组痘苗病毒载体,为研制宫颈癌治疗性疫苗奠定基础.     

CSFV E2蛋白主要抗原区域在马克斯克鲁维酵母中的表达及小鼠免疫

猪瘟是一种由猪瘟病毒(Classical swine fever virus,CSFV)引起的高度接触传染性和致死性疾病.疫苗预防接种是控制猪瘟病毒传播的重要措施,目前我国使用的弱毒疫苗均为1.1亚型猪瘟病毒兔化弱毒株.随着病毒的不断变异,2.1亚型猪瘟病毒已经逐渐成为我国的主要流行毒株.本研究利用马克斯克鲁维酵母研究了2.1b型CSFV E2蛋白及其截短体mE2(690-916aa)在马克斯克鲁维酵母中的重组表达,发现去除E2蛋白跨膜区域可以显著提高E2蛋白表达水平,mE2的表达量在5L发酵罐中达1.25～2.5g/L.经分子筛纯化,mE2蛋白纯度达90%.纯化的mE2作为抗原蛋白,注射免疫小鼠28d后,可诱导小鼠产生抗CSFV的IgG特异性抗体,表明马克斯克鲁维酵母重组表达的mE2蛋白具有较好的免疫原性.     

烷烃、醇、醚的定量结构与保留相关性(QSRR)研究

在邻接矩阵的基础上 ,建立一种新的连接性指数 mL =∑(δi·δj·δk·… ) 0 .5,其中 :0 L =∑(δi) 0 .5,1L =∑(δi·δj) 0 .5,并计算了 146个有机分子的0 L、1L值 .发现 mL不仅对这些化合物具有良好的结构选择性 ,而且与其气相色谱保留指数RI具有很好的相关性 .     

价电子轨道能量拓扑指数mE及其对P区氢化物酸性的预测

受Randic分子连接性拓扑指数mX的启发,构建了一种价电子轨道能量拓扑指数mE.利用其0阶(0E)和1阶(1E)指数,与13种p区无机氢化物的pKa1关联,拟合成2个线性回归方程.拓扑指数的结构选择性达到唯一性表征,相关系(指)数为0.9942(0.9949).预测取得了令人满意的结果.     

mG 用于醚结构/性质相关性研究

在分子拓扑理论基础上,提出一个新的连接性指数 mG,mG=∑(Ai*Aj*Ak…)0.5,其中0G=∑(Ai)0.5,1G=∑(Ai*Aj)0.5,并用0G、1G 与醚的摩尔折射、沸点、气相色谱保留指数等进行关联,相关系数分别为0.999 4,0.991 9,0.999 6.本方法具有物理意义明确,计算简单,使用方便等优点.     

共价氢化物在水溶液中的pka值的定量探讨

本文定义元素的能级连接性指数E为1E=∑(δi×δj)-0.5,δ为最低空原子轨道主量子数/最高占有原子轨道能量(-eV)(或最高占有原子轨道主量子数/最低空原子轨道能量(-eV)).1E均对16种P区氢化物呈现唯一性表征,并与其水溶液中的pka值呈一元线性关系.本文预测了16种p区氢化物在水溶液中的pka值,其计算值和实验值是很吻合或很接近的.     

鲁金定理的一个简单证明

E是N维欧氏空间R~N 中的一个L可测集,其测度为mE<∞或mE=∞.现行教材中,关于鲁金定理的证明大多以叶果洛夫定理为工具,而叶果洛夫定理仅在mE<∞时才成立,因而鲁金定理的证明就必需分成两步,先对mE<∞的情况进行证明,再对mE=∞的情况进行证明.在复旦大学的教材〔1,131页〕中,鲁金定理的证明虽然未引用叶果洛夫定理,但其证明方法仍必需分成mE<∞和mE=∞两种情况进行证明.本文改进了中的证明方法,只需一步完成证明,使之无论对mE<∞或mE=∞都成立,而且证明的方法既初等又简单,在教学中可以采用.     

用边邻接指数预测链烷烃的热力学性质

用分子图理论和边价 (δei)建构新的连接性指数 (mE) ,籍以描述分子的大小及分支情况 .其中 ,0 E、1 E和碳原子的最大支化度 (δmax)或路径数P3 与 85种链烷烃的标准生成焓 (ΔfHθm)、标准熵 (Sθm)、标准生成自由能 (ΔfGθm)具有良好的相关性 :-ΔfHθm =5 1.12 0 7+2 .0 6 0 2 0 E+38.0 746 1 E+8.5 116δmax,R =0 .9979,Sθm =179.4143+35 .18840 E +2 8.0 3981 E - 1.0 0 3 4δmax,R =0 .9988,ΔfGθm =- 42 .4941+2 .5 330 0 E +9.38131 E +2 .12 5 9P3,R =0 .993 6 .     

mB 对气相色谱保留指数的QSPR研究

在邻接矩阵的基础上,建立一种新的连接性指数 mB mB =∑( δi*δj*δk …)0.5,其中 0 B =∑( δi) 0.5,1 B =∑( δi*δj )0.5,并计算出114个分子的 0B、1B值.发现mB 与这些化合物的气相色谱保留指数 RI 有很好的相关性.烷烃的线性回归方程为 RI=25.607 3+83.856 10B-9.278 41B,相关系数R=0.996 3;醇的线性回归方程为RI=27.013 8+130.022 90B-41.048 61B,相关系数R=0.997 6;取代苯的线性回归方程为RI=-5.088 9+22.457 50B-24.098 61B,相关系数R =0.992 8;分子连接性指数能较好地反映化合物的结构特征.     

取代苯甲醇和氯代苯的定量结构—活性关系研究

定义表征原子生物活性的特征值Ai,由Ai建构新的价连接性指数 nH=∑(Ai*Aj*Ak…),其中0阶指数 0H=∑(Ai),1阶指数 1H=∑(Ai*Aj).0H、1H与取代苯甲醇对黑曲霉素的生物活性及氯代苯对鲤鱼、呆鲦鱼和发光菌的急性毒性均具有高度的相关性.本文所需参数易得,计算方法简单,预测结果满意.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that