On the formation of corpus balbiani in the ovogonia of man and guinea-pig

Summary Studies were conducted on the ovogonia of human embryos at the age of 33, 35 and 40 days. Myelin-like bodies in the nuclei and in the cytoplasm were observed. In other cases, the ovogonium contained 4–5 myelin-like bodies oriented round the centrioles. This is supposed to represent an early phase of corpus balbiani formation.     

Effect of N-6 2'-O-dibutyryl adenosine 3'-5'monophosphate (dibutyryl cyclic AMP) on the condenstion of the X chromosome into the form of a Barr body]

Addition of exogen DB-c AMP in medium of human fibroblasts culture, with 46 XX karyotypes, enables us to observe a high frequency of Barr bodies, about 80 or 85%. This observation suggests that the condensation of the X chromosome in the form of Barr bodies depends on the level of endogen cyclic AMP.     

Organization of the oocyte nucleus in silphid beetles (Silphidae,Coleoptera-Polyphaga)

Summary In all the Silphid species examined, the oocyte chromosomes were found to, contract to form the karyospheres. Despite this, as the oocytes grow the germinal vesicles increase also very considerably. Production of numerous RNA-containing nucleolus-like bodies was found to be characteristic of the Silphid oogenesis. Experiments with³H-uridine demonstrated that only the karyosphere actively synthesized RNA, while the nucleolus-like bodies remained inactive in this process.This investigation was supported in part under Contract DPKBN/52/76-II. 1.3.10. with the Polish Academy of Science.     

Phospholipid and fatty acid composition of lamellar bodies isolated from chicken lungs at different stages of development]

In order to elucidate whether the lamellar body content represents functional surfactant as soon as these bodies appear in lung epithelium during fetal development, lamellar bodies were isolated from lungs of Chicken at various developmental stages. Their content was analyzed and compared to extra-cellular functionnal surfactant. The results show that this content undergoes gradual changes in the phospholipid/protein ratio, as well as in the relative phosphatidylcholine amount. Thus, in the Chicken, nascent lamellar bodies do not represent the final form of extracellular surfactant.     

Uptake and transport of exogenous peroxidase in the masticatory muscles of the cat. Localization of motor neurons]

In the present work, a topographical localization of masticatory muscle motoneurons was undertaken. Horseradish peroxydase injected in each muscle can be transported in the retrograde direction to the corresponding motoneurons cell bodies. Jaw-closing muscle motoneurons were identified in the dorsal part of the motor trigeminal nucleus whereas jaw-opening muscle motoneurons were observed in the ventro-medial region.     

Analysis of nuclear apoptotic process in a cell-free system

We report an analysis of the apoptotic process of mouse liver nuclei induced in a cell-free carrot cytosol system by cytochrome c. Typical characteristics of apoptosis were observed, such as chromatin condensation, margination, apoptotic bodies and DNA ladders. Furthermore, transmission and scanning electron microscope analysis of the apoptotic nuclei detected chromatin-free nuclear vesicles before apoptotic bodies appeared at a comparatively late phase. When AC-YVAD-CHO, an inhibitor of caspase 6, was introduced into the system, these vesicles and apoptotic bodies disappeared completely within our study sections. We confirmed the results using whole-mount electron microscopy, and found that although the nuclear lamina was destroyed early, the nuclear matrix largely remained intact during the course of apoptosis. The nuclear matrix played an important role in maintaining the integrity of apoptotic cells and connecting the apoptotic bodies and apoptotic nucleus. Received 29 September 2000; revised 10 December 2000; accepted 13 December 2000     

Ovulation and the role of the ovarian surface epithelium.

Epon sections from all tissue layers of the rabbit ovary, including the often neglected surface or germinal epithelium, were studied to elucidate the mechanism of follicle rupture. Scanning electron microscopy showed cells covering preovulatory follicles increased clearly in size up to 8 hours after human chorionic gonadotropin (HCG) injection. These showed an increasing number of large, intracellular structures with prominent rounded contours. Transmission electron microscopy showed large, electron dense, lysosomelike bodies in some cells 4 hours after HCG. These membrane-surrounded structures increased in size up to 8 hours after HCG, then decreased markedly. These obviously corresponded to the bodies found by light microscopy and scanning electron microscopy. Histochemistry revealed many represent lysosomes. During the last 2 hours before rupture, the dense bodies of the surface epithelium considerably decrease, signs of material emptying into vacuoles is found, and sometimes there is open communication from vacuoles towards the unerlying tunica albuginea. An extracellular edema appeared under the epithelium with degenerated fibroblasts and disintegrated collagen; these changes gradually proceeded inwards. Blood capillaries close to the membrana granulosa gradually showed small pores and close to ovulation the endothelial cells had gaps up to 3 mcm in diameter. The preovulatory follicles grew rapidly and an augmenting edema occupied the whole ovary. In the last hours before ovulation the membrana granulosa gradually dissociated. Whatever the net effect of the prostaglandins, prostaglandin F2alpha appears to be essential for follicle rupture since intrafollicular injections of antiserum blocked ovulation. Prostaglandin E1 promotes vascular permeability and lysosommal enzymes released extracellularly may be coupled to collagen degration. The enzyme synthesis and lysosomal growth in the surface epithelium of preovulatory follicles may be due to the high local concentration of sex steroids.     

Immunocytochemical localization of GnRH (gonadotropin releasing hormone) systems in the brain of a marine teleost fish,the sole

Summary The GnRH system was studied in the brain of the sole by immunocytochemistry (peroxidase-antiperoxidase method) (PAP) using antibodies to synthetic salmon GnRH (s-GnRH). Two centers containing immunoreactive cell bodies were observed in the forebrain, one located at the junction between the olfactory bulbs and the telencephalon and the other in the preoptic area. Numerous immunoreactive fibers were found, especially in the telencephalon, hypothalamus, pituitary, optic tectum and retina.     

Proving the principle: Taking geodesic dynamics too seriously in Einstein's theory

In this paper I critically review attempts to formulate and derive the geodesic principle, which claims that free massive bodies follow geodesic paths in general relativity theory. I argue that if the principle is (canonically) interpreted as a law of motion describing the actual evolution of gravitating bodies, then it is impossible to generically apply the law to massive bodies in a way that is coherent with Einstein's field equations. Rejecting the canonical interpretation, I propose an alternative interpretation of the geodesic principle as a type of universality thesis analogous to the universality behavior exhibited in thermal systems during phase transitions.     

Virus-like particles in the opossum submandibular gland

Summary Numerous inclusion bodies (virus-like particles) were observed in the lumina of the intercellular canaliculi, mucous tubules and intralobular ducts of the opossum submandibular gland. The particles are spherical in outline, show an electron dense core, and are surrounded by a peripheral membrane.     

‘Spuntar lo scoglio più duro’: did Galileo ever think the most beautiful thought experiment in the history of science?

Still today it remains unclear whether Galileo ever climbed the leaning tower of Pisa in order to drop bodies from its top. Some believe that he established the principle of equal speeds for falling bodies by means of an ingenious thought experiment. However, the reconstruction of that thought experiment circulating in the philosophical literature is no more than a cartoon. In this paper I will tell the story of the thought processes behind the cartoon.     

Locomotor behavior in relation to octopamine levels in the antLasius niger

Summary The heads and bodies of hyperactive and hypoactive ants, selected on the basis of motor activity, were tested for their octopamine content. The level was found to be significantly higher in hyperactive animals. A possible involvement of octopamine in locomotor behavior is discussed.     

Acid phosphatase in eggs of the zebrafish,Brachydanio rerio

Summary 2 isozymes of acid phosphatase have been identified by polyacrylamide disc gel electrophoresis in the ovary and mature, unfertilized eggs ofB. rerio. Histochemically, the enzyme appears to be localized in preyolk bodies of previtellogenic oocytes and in yolk platelets of vitellogenic and postvitellogenic oocytes. The contents of the cortical granules at all stages of oocyte differentiation were acid phosphatase negative.     

The presence of para-esophageal bodies in Nematophora Lysiopetalidae (Myriapoda, Diplopoda)]

Demonstration of paraoesophageal bodies presence in the Millipede Nematophora Lysiopetalidae, Callipus foetidissimus (Savi, 1819). As in the Iulidae, Blaniulidae, Strongylosomidae and Glomeridia the paraoesophageal bodies are neurohemal organs having their own secretory cells. Different hypothesies on the origin of secretions in the paraoesophageal bodies of Lysiopetalidae are proposed.     

Spinoza on the resistance of bodies

Commentators often claim that the bodies of Spinoza’s physics resist the changes they undergo. But it’s not always clear what they mean when they say this, or whether they are entitled to say it. This article clarifies what it might mean to for Spinoza’s bodies to resist change, and examines the evidence for such a view. In the first half, the author argues that there is some limited evidence for such a view, but not nearly as much as people think. In the second half, the author proposes looking for a mental analogue to collision in the realm of ideas and argues that adequacy amounts to a meaningful concept of resistance in Spinoza, albeit one that is incomplete.     

The role of zinc in the activation of the enzyme cellulase in termites

Summary Chemical analysis had revealed an exceptionally high amout of zinc in the worker bodies of termites. Further investigation of the digestion of cellulose in vitro demonstrated that zinc functions as activator of the enzyme cellulase.     

Histochemical localization of acetylcholinesterase in the glycogen body (sinus rhomboidalis) of common brown dove,Streptopelia senegalensis and house sparrow,Passer domesticus

Summary Acetylcholinesterase (AChE) activity was studied in the glycogen bodies of the spinal cords of 2 birds namelyStreptopelia senegalensis andPasser domesticus. A possible functional significance of AChE in the light of relative enzymatic localization especially in Hoffmann-Kolliker nuclei (motor cell groups), substantia gelatinosa and other regions of gray matter of 2 avian glycogen bodies has been discussed.     

An immunohistochemical study of the clearance of apoptotic cellular fragments

We investigated the distribution and fate of apoptotic bodies during human development and in the adult, using an antibody (M30) that recognizes a neo-epitope formed early in the apoptotic cascade by caspase cleavage of cytokeratin 18. In the fetus, we found extensive accumulation of M30-positive, non-phagocytosed fragments in the red pulp of the spleen, subcutaneous and submucosal vessels, the interstitium of the lung, and the glomerular mesangium of the kidneys. In the liver, M30-immunoreactive fragments were found inside macrophages in the sinusoids. The number of these fragments and the intensity of the immunostaining increased with the gestational age of the fetus. In the adult, M30-positive fragments were barely detectable in normal tissues. However, many pathological situations, including both chronic degenerative processes and metastatic cancer, were associated with accumulation of M30-positive fragments in the red pulp of the spleen. In the liver and kidney, no fragments could be detected. Remarkably, 13 of the 16 patients with metastasized cancer showed pronounced accumulation of M30-positive fragments containing hematoxylin-reactive material in the red pulp of the spleen. In the non-cancerous cases, such DNA-containing fragments were only seen in 9 of 94 cases. The results show that when apoptotic activity is high, as during development in the fetus or during metastasis and other pathological processes in the adult, the phagocytic clearance of apoptotic bodies can be overloaded. These apoptotic fragments then accumulate in the spleen. The visual detection of apoptotic fragments is concluded to reflect increased cell turnover. Received 1 July 2002; accepted 1 July 2002     

Electron microscopical evidence of the evolution of corynebacteria-like microorganisms within human erythrocytes

The corynebacteria-like microorganisms evoluting in the haemocultures take origin from electron dense granular bodies carried within the erythrocytes.     

Electron microscopical evidence of the evolution of corynebacteria-like microorganisms within human erythrocytes

Summary The corynebacteria-like microorganisms evoluting in the haemocultures take origin from electron dense granular bodies carried within the erythrocytes.