共查询到20条相似文献,搜索用时 15 毫秒
1.
Acrylamide and its related compounds inhibited brain enolases in vitro independently of their neurotoxicity. The inhibitory potency was a function of the binding constants of the compounds for phenylalanine. The binding constant for tryptophan was higher in neurotoxic compounds than in non-neurotoxic ones. 相似文献
2.
Summary The activity of liver and brain transglutaminase is rapidly lost following i.p. injection of acrylamide (50–200 mg/kg). Other enzymes investigated were not modified by the treatment, with the exception of brain enolase. 相似文献
3.
G. Bartholini A. Pletscher K. F. Gey 《Cellular and molecular life sciences : CMLS》1961,17(12):541-542
Riassunto Cloropromazina, imipramina, cloroprotixene e amitriptilinain vitro diminiscono la 5-idrossitriptamina (5HT) endogena nei trombociti di coniglio e, per quanto si è visto, anche in quelli umani. Nel plasma di coniglio pretrattato con isocarbossazide, inibitore della mono-amino-ossidasi, si osserva un corrispondente aumento di 5HT. Cocaina, -metil-dopa e triptamina hanno un effetto meno pronunciato sulla 5HT delle piastrine.
Guest worker from the Istituto di Patologia sp. Medica dell'Università di Bologna (Italy). 相似文献
Guest worker from the Istituto di Patologia sp. Medica dell'Università di Bologna (Italy). 相似文献
4.
K. Oomagari H. Uchimura T. Matsumoto H. Yokoo M. Hirano J. S. Kim T. Nakahara 《Cellular and molecular life sciences : CMLS》1984,40(11):1288-1290
Summary Serotonin and 5-hydroxyindoleacetic acid (5-HIAA) were measured in individual nuclei of rat hypothalamus and other brain areas using HPLC with electrochemical detection. 5-HIAA levels were first demonstrated in hypothalamic and some discrete brain areas. The 5-HIAA/5-HT ratio was highest in the n. caudatus putamen, high in the n. ventromedialis and lowest in the n. suprachiasmaticus. 相似文献
5.
6.
Summary Ablation of the brain from hibernatingHelix aspersa maintained at 25°C causes a significant increase in the proliferation of male cells in the gonad, whereas the ablation of the optic tentacles has no effect. The brain, therefore, produces a factor which specifically inhibits the multiplication of spermatogonia and spermatocytes. 相似文献
7.
L. -J. C. Wong G. -C. Tsao J. I. Bruce S. S. Wong 《Cellular and molecular life sciences : CMLS》1990,46(5):461-464
Summary The schistosomicides, hycanthone, oxamniquine and praziquantel, were found to inhibit the in vitro RNA synthesis using isolated hamster liver nuclei. Preincubation of the nuclei with these drugs revealed that the inhibitory effect of oxamniquine was irreversible and progressed with time, whereas that of hycanthone and parziquantel was reversible. On the other hand, hycanthone and praziquantel have a high affinity for DNA but oxamniquine does not. The data indicate that the mechanism of inhibition by oxamniquine is different from that of hycanthone and praziquantel. 相似文献
8.
The schistosomicides, hycanthone, oxamniquine and praziquantel, were found to inhibit the in vitro RNA synthesis using isolated hamster liver nuclei. Preincubation of the nuclei with these drugs revealed that the inhibitory effect of oxamniquine was irreversible and progressed with time, whereas that of hycanthone and praziquantel was reversible. On the other hand, hycanthone and praziquantel have a high affinity for DNA but oxamniquine does not. The data indicate that the mechanism of inhibition by oxamniquine is different from that of hycanthone and praziquantel. 相似文献
9.
E. Habermann 《Cellular and molecular life sciences : CMLS》1988,44(3):224-226
Summary Rat brain homogenate was preloaded with [3H]noradrenaline or [3H]GABA and stimulated with high K+. Tetanus toxin and botulinum A neurotoxin partially prevent the evoked [3H]noradrenaline release in the same range of toxin concentrations starting below 10–10M. In contrast, release of -amino butyric acid (GABA) is much more sensitive to tetanus than to botulinum A toxin. 相似文献
10.
11.
A relevant in vitro rat model for the evaluation of blood-brain barrier translocation of nanoparticles 总被引:3,自引:0,他引:3
Garcia-Garcia E Gil S Andrieux K Desmaële D Nicolas V Taran F Georgin D Andreux JP Roux F Couvreur P 《Cellular and molecular life sciences : CMLS》2005,62(12):1400-1408
Poly(MePEG2000cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles have demonstrated their capacity to reach the rat central nervous system after intravenous injection. For insight into the transport of colloidal systems across the blood-brain barrier (BBB), we developed a relevant in vitro rat BBB model consisting of a coculture of rat brain endothelial cells (RBECs) and rat astrocytes. The RBECs used in our model displayed and retained structural characteristics of brain endothelial cells, such as expression of P-glycoprotein, occludin and ZO-1, and immunofluorescence studies showed the specific localization of occludin and ZO1. The high values of transendothelial electrical resistance and low permeability coefficients of marker molecules demonstrated the functionality of this model. The comparative passage of polyhexadecylcyanoacrylate and PEG-PHDCA nanoparticles through this model was investigated, showing a higher passage of PEGylated nanoparticles, presumably by endocytosis. This result was confirmed by confocal microscopy. Thanks to a good in vitro/in vivo correlation, this rat BBB model will help in understanding the mechanisms of nanoparticle translocation and in designing new types of colloidal carriers as brain delivery systems.Received 4 March 2005; accepted 14 April 2005 相似文献
12.
H. Tsuchiya M. Sato M. Iinuma J. Yokoyama M. Ohyama T. Tanaka I. Takase I. Namikawa 《Cellular and molecular life sciences : CMLS》1994,50(9):846-849
Phytoalexins, defensive compounds produced by plants against microbial infections, were purified fromSophora exigua (Leguminosae) and their growth inhibitory effects on oral cariogenic bacteria were determined in vitro. Among three isolated compounds, 5,7,2,4-tetrahydroxy-8-lavandulylflavanone completely inhibited the growth of oral bacteria including primary cariogenic mutans streptococci, other oral streptococci, actinomycetes, and lactobacilli, at concentrations of 1.56 to 6.25 g/ml. 相似文献
13.
Summary The distribution of immunoreactive LH in the brain of the white-footed mouse (Peromyscus leucopus) was determined using immunocytochemical procedures. Immunoreactive fibers are located in the hypothalamus, preoptic area, septum and amygdala. Stained cell bodies are seen in the arcuate nucleus and preoptic area. Gonadectomy enhances staining for LH in the brain.We wish to thank the National Institutes of Arthritis, Metabolism, and Digestive Diseases (NIAMDD) for the gift of the antiserum to LH. 相似文献
14.
15.
Zusammenfassung Die Hemmung der cAMP-Phosphodiesterase in verschiedenen Organen der Katze durch Hydergin und DHE wurde in vitro bestimmt. In Konzentrationen von 10–4
M bis 10–6
M wurde ein Hemmeffekt nur im Gehirn beobachtet. Diese Wirkung wird besonders deutlich, wenn man das infolge der Enzymhemmung nicht umgesetzte cAMP berechnet. 相似文献
16.
A convulsant, 3-mercaptopropionic acid,decreases the level of GABA and GAD in rat pancreatic islets and brain 总被引:1,自引:0,他引:1
J. Katoh H. Taniguchi M. Ogura M. Kasuga Y. Okada 《Cellular and molecular life sciences : CMLS》1995,51(3):217-219
To investigate the properties of the gamma-aminobutyric acid (GABA) synthesizing enzyme, glutamate decarboxylase (GAD), in the brain and the pancreatic islets of the rat, GABA concentration in the brain and the pancreatic islets was measured after intraperitoneal administration of 3-mercaptopropionic acid (3-MP) at 25 mg/kg. 60 min after the administration of 3-MP, GABA concentration in the hypothalamus, the superior colliculus and the hippocampus of the brain decreased by 20–30% and in the pancreatic islets by 35%. The concentration in the pancreatic acini did not change. Western blotting showed that GAD activity in the pancreatic islets decreased after administration of 3-MP compared to the control. The activity of GAD in the pancreatic islets as well as brain can be modified by a convulsant, in this case 3-MP. These results suggest the properties of GAD may be similar in the pancreatic islets and brain. 相似文献
17.
A. Velasco Martín J. M. Arévalo Alonso M. de Armijo Valenzuela 《Cellular and molecular life sciences : CMLS》1972,28(8):934-934
Resumen La mecloqualona inhibe in vitro, la respiración de cortes y homogenizados de cerebro, incrementando el consumo de glucosa; este último efecto no se produce cuando al medio de incubación se añaden 100 mM de potasio. 相似文献
18.
W. Lippmann 《Cellular and molecular life sciences : CMLS》1977,33(1):61-63
Summary Various somatostatin (S) analogs exhibited similar degree and similar, or shorter, duration of inhibition of basal gastric acid secretion as S in the unanesthetized rat and similar, or less, inhibition of the cyclic AMP accumulation induced by prostaglandin E2 in the rat anterior pituitary in vitro. With the analogs examined, the gastrointestinal and pituitary receptors appear to exhibit generally similar recognition specificity with the differences within the gastrointestinal activities reflecting duration of availability rather than receptor affinity.The author acknowledges the technical assistance of Mr L. Chamberland, Miss A. Colaviti and Miss C. Pilapil. 相似文献
19.
W. Kozak D. Soszynski M. Szewczenko M. Bodurka 《Cellular and molecular life sciences : CMLS》1990,46(10):1010-1011
Summary Successive injections of lipopolysaccharide (LPS) either intravenously (i.v.) or intracerebroventricularly (i.c.v.) induced pyrogenic tolerance to LPS in rabbits. Tolerance was shown by a decrease of the magnitude of the fever response to repeated doses of LPS, irrespective of the route of pyrogen administration. A significantly greater and more dramatic decrease of the fever index, however, was observed in rabbits made tolerant to pyrogen given i.v. than when the pyrogen was given i.c.v. Transmission of the pyrogenic toleraance between brain and peripheral tissues, however, has not been ascertained. 相似文献