甘蔗糖蜜发酵产黄原胶优良菌株的选育

黄原胶是由野油菜黄单胞菌发酵生产的重要的商业微生物多糖产品,实验通过微波诱变处理保藏菌株,筛选到一株能以甘蔗糖蜜为碳源发酵生产黄原胶的优良正突变株X12,实验结果表明：在初步确定的实验条件下,该菌株发酵生产黄原胶的产量达22.14 g/L,是未诱变菌株20183的1.53倍,传代实验表明,突变菌株X12的黄原胶产量及性状无大变化,遗传性状稳定。     

福建甘蔗废糖蜜生物合成L-赖氨酸的研究

本文研究了以福建甘蔗糖蜜为原料生物合成L—赖氨酸。探索福建甘蔗糖蜜的化学成分、发酵培养基组成和培养条件对L—赖氨酸积累的影响,并进行扩大试验。结果表明:糖蜜比淀粉作为发酵培养基的碳源,其L—赖氨酸的产酸率较高。从扩大试验获得的精制品含L—赖氨酸98.9％。说明福建甘蔗糖蜜完全可以利用为碳源生产L—赖氨酸。本研究为福建糖蜜的利用开辟一条新的途径。     

野油菜黄单胞菌X.c—18的选育及发酵条件的研究

通过人工诱变野油菜黄单胞菌，筛选出１株具有良好抗变异性的突变株Ｘ．ｃ－１８。该突变株能很好地利用蔗糖和玉米淀粉为碳源，蛋白胨和黄豆饼粉为氮源。其最佳黄原胶发酵总糖量与总蛋白质量之比为５：０．５，最适ｐＨ值为７．０，温度为２８℃。摇瓶发酵７２ｙ，黄原胶产量每升发酵液一般可在３０ｇ以上，最高产量达３２．６ｇ，对碳源转化率为７１．５４％。     

两步发酵法生产黄原胶的研究

黄原胶生产中发酵过程的氧限制及分离工序的高醇耗是造成高能耗,高成本的主要原因,作者探讨了一种生产高原胶的新途径－两步发酵法,可以解决上述两个问题,方法的主要特点是首先在一级罐中培养黄单胞菌以产生合成黄原胶的胞外酶,于利用这些胞外酶在二级罐中以无菌的底物发酵生产黄原胶,发酵在膜式两级发酵罐中进行,确定了最优发酵条件,取得了理想的结果,产胶量比普通法提高２４．５％,直接获得无菌体的发酵产物,简化了分离     

甘蔗糖蜜酒精高产酵母的发酵特性研究

采用20L三联装发酵罐进行甘蔗糖蜜高浓度酒精发酵研究高产酿酒酵母(Saccharomyces cerevisiae)MF1001菌株的甘蔗糖蜜酒精发酵特性。结果表明,锤度为20°Bx的糖蜜对菌株生长和发酵均没影响,发酵的适宜温度为30℃,pH值4.0的发酵效果明显优于pH值3.80,传代培养16代及不同的接种量对菌株的发酵没有影响。按目前甘蔗糖蜜酒精生产的发酵工艺,用锤度为20°Bx糖蜜培养基培养菌株,制备种子液,然后将种子液与锤度为55°Bx的糖蜜培养基1∶1混合进行发酵,发酵50h的醪液酒精含量达到了13.2%(V/V),60h达13.8%(V/V),72h达14.3%(V/V)。发酵结束时醪液可发酵残糖含量低至0.44%,发酵效率分别为理论值88.6%(50h)、94.3%(60h)和98.6%(72h)。该菌株有望用于甘蔗糖蜜酒精发酵生产,提高甘蔗糖蜜酒精发酵的醪液酒精含量。     

甘蔗糖蜜促进类球红细菌辅酶Q10的糖转化效率

考察了甘蔗糖蜜对类球红细菌发酵生产辅酶Q₁₀生物合成的影响,优化得到最佳的甘蔗糖蜜添加工艺,提升了糖转化率,大幅度降低了生产成本。不同碳源底物对辅酶Q₁₀产物合成有较大的影响,其中葡萄糖为最适碳源,利于类球红细菌菌体的生长和辅酶Q₁₀的合成。通过正交试验对辅酶Q₁₀发酵培养基进行了优化,当甘蔗糖蜜质量浓度为20 g/L、葡萄糖质量浓度为30 g/L、KH₂PO₄质量浓度为1 g/L时,对类球红细菌生长和辅酶Q₁₀合成均有利,且辅酶Q₁₀发酵效价达到了380.1 mg/L,糖转化率12.71 mg/g。在30 L发酵罐上进行发酵放大,结果辅酶Q₁₀产量最高达到3 311 mg/L,较对照组提高了15.4%,糖转化率提高了13.9%,这些发酵过程的生理参数进一步验证了甘蔗糖蜜有利于菌体代谢活力的维持以及辅酶Q₁₀的合成。     

甘蔗糖蜜生产兰姆酒的试验报告 第一报 菌种特性的比较试验

兰姆酒是以甘蔗糖蜜或甘蔗汁为原料经发酵、蒸馏而制成具有特殊风味的一种世界各酒,结合我国华南地区盛产甘蔗的特点,利用甘蔗糖蜜发酵生产兰姆酒,这对扩大甘蔗糖蜜的利用有重大意义。我们搜集了几种国内外适于甘蔗糖蜜发酵的酵母菌种和丁酸菌种进行了一系列形态和生理特性的试验,以便选育出能产生兰姆酒独特香味和风味的优良菌种。现将试验结果总结如下:     

黄原胶适宜培养条件的研究

分别研究了野油菜黄单胞菌ＳＤ－２液体种子培养和发酵产胶的适宜条件。液体种子培养的适宜条件为：起始ｐＨ７．０，ＣａＣｏ３ｉ浓度０，培养温度２９℃，５００ｍＬ三角瓶装量１００ｍＬ，发酵产胶的适宜条件为：起始ｐＨ７．，ＣａＣｏ３浓度０．２％，培养温度３１℃，５００ｍＬ。采用适宜条件发酵黄原胶，最终发酵液粘度为８７２０ｍＰａ．ｓ，黄原胶浓度为２．７８％，分别比对照提高了２５．３％和１５．４％。     

甘蔗糖蜜发酵生产维生素B12的动力学模型

为明确甘蔗糖蜜发酵生产维生素B12的过程,在优化过的培养工艺基础上,以甘蔗糖蜜作为培养基,研究利用谢氏丙酸杆菌发酵生产维生素B12的动力学特性,通过利用Origin7.5软件对试验数据进行回归分析,采用Logistic方程、Luedeking-Piret方程和与Luedeking-Piret方程相似的基质消耗方程对发酵...     

溶氧对黄原胶发酵的影响

黄原胶是野油菜黄单孢菌分泌的胞外多糖,可用淀粉或葡萄糖为碳源发酵生产。本文着重以淀粉为碳源研究不同的溶氧水平对多糖的影响。试验结果表明:溶氧水平高于饱和度的20%对多糖发酵影响不大,低于20%时菌的生长受影响。溶氧水平为饱和度的25%,菌体生长最佳。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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